Method for preparing polyvinylidene fluoride affinity membrane using amino acid as ligand

A polyvinylidene fluoride and amino acid technology, which is applied in the preparation method of peptides, chemical instruments and methods, membrane technology, etc., to achieve the effect of simple and convenient process, high adsorption efficiency, and meeting the needs of clinical applications

Inactive Publication Date: 2009-12-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Endotoxin adsorbent for hemoperfusion with polyvinylidene fluoride membrane as carrier has not been reported

Method used

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  • Method for preparing polyvinylidene fluoride affinity membrane using amino acid as ligand
  • Method for preparing polyvinylidene fluoride affinity membrane using amino acid as ligand
  • Method for preparing polyvinylidene fluoride affinity membrane using amino acid as ligand

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Rinse the PVDF hollow fiber membrane with 30% ethanol for 30 minutes, and soak it in deionized water for 1 hour to wash away impurities on the surface of the membrane.

[0040] 2) Add 40g / l of KMnO 4 and 3mol / l KOH mixed solution at 80°C with a constant flow pump into the PVDF hollow fiber membrane module to react for 30min, and then with 60g / l H 2 SO 4 and 60g / l NaHSO 3 The mixture is restored.

[0041] 3) The membrane after reduction treatment was treated with a mixture of 1 mol / l NaOH and 10 g / l HEC at 90° C. for 15 min to increase the hydrophilicity of the membrane surface. Place it in a constant temperature hot air drying oven at 90°C and dry it with 0.5mol / l Na 2 CO 3 The solution was washed at 90°C for 10 min to wash off the unreacted HEC on the surface of the PVDF membrane.

[0042] 4) The hydrophilically modified membrane was activated at 60° C. with a mixture of epichlorohydrin (ECH): 1 mol / l NaOH with a volume ratio of 1:4, and washed with deionized ...

Embodiment 2

[0050] 1) Rinse the PVDF hollow fiber membrane with 30% ethanol for 40 minutes, and soak it in deionized water for 1 hour to wash away impurities on the surface of the membrane.

[0051] 2) Add 50g / l of KMnO 4 and 3mol / l KOH mixed solution at 60°C with a constant flow pump into the PVDF hollow fiber membrane module to react for 30min, and then with 60g / l H 2 SO 4 and 60g / l NaHSO 3 The mixture is restored.

[0052] 3) The membrane after reduction treatment was treated with a mixed solution of 1.5 mol / l NaOH and 15 g / l HEC at 80° C. for 10 min to increase the hydrophilicity of the membrane surface. Place it in a constant temperature hot air drying oven at 80°C and dry it with 0.5mol / l Na 2 CO 3 The solution was washed at 80°C for 10 min to wash off the unreacted HEC on the surface of the PVDF membrane.

[0053] 4) The hydrophilized modified membrane was activated with a mixture of epichlorohydrin (ECH): 1 mol / l NaOH with a volume ratio of 1:3 at 50° C., and washed with dei...

Embodiment 3

[0061] Step 1-6 is the same as embodiment 1

[0062] 7) React with 3g / l asparagine solution (0.2mol / l phosphate buffer with pH 7.2 as solvent) at 45°C for 24h to obtain polyvinylidene fluoride grafted with asparagine ligand membrane.

[0063] The experimental procedure for the removal of endotoxin in plasma by the polyvinylidene fluoride affinity membrane with asparagine ligand is the same as that in Example 1, and the graft density and endotoxin removal efficiency of the ligand are shown in Table 3.

[0064] The performance of the prepared affinity membrane of table 3 embodiment 3

[0065]

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Abstract

The invention discloses a method for preparing a polyvinylidene fluoride affinity membrane using amino acid as a ligand. The affinity membrane is prepared by hydrophilically modifying a polyvinylidene fluoride hollow fiber membrane and grafting the amino acid ligand. The grafting amount of the amino acid is 150 to 250mg/g for each membrane. The invention also discloses application of the affinity membrane in removing endotoxin in blood plasma, which removes the endotoxin in the blood plasma by a dynamic absorption means. The polyvinylidene fluoride affinity membrane prepared by the method has stable performance, good bio-compatibility and high endotoxin removing efficiency, and can be used for whole blood perfusion as well as blood plasma perfusion.

Description

technical field [0001] The invention relates to a preparation method of a polyvinylidene fluoride affinity membrane with amino acid as a ligand, in particular to a method for preparing a polyvinylidene fluoride affinity membrane for removing blood plasma through hydrophilic modification and grafting of an amino acid ligand to a polyvinylidene fluoride hollow fiber membrane Polyvinylidene fluoride affinity membrane method for endotoxin. Background technique [0002] At present, endotoxemia has three high phenomena of high morbidity, high mortality, and high treatment costs, which have constituted a serious threat to human health and a huge burden on economic development. Endotoxin is one of the structural components of the outer membrane of the cell wall of Gram-negative bacteria, and its chemical essence is lipopolysaccharide (LPS). Since the intracellular signal transduction of cell activation induced by LPS is extremely complex, once the cascade effect initiated by the bi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D71/82B01D71/78B01D71/34B01D69/08A61M1/34C07K1/22C07K1/34
Inventor 张林叶茜徐堃陈欢林徐秋萍黄曼
Owner ZHEJIANG UNIV
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