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Total cholesterol detection reagent and total cholesterol detection paper

A detection reagent and detection test paper technology, applied in the field of total cholesterol detection reagent and total cholesterol detection test paper, can solve the problem of insufficient detection speed, and achieve the effects of fast and accurate detection, rapid detection and accurate detection

Active Publication Date: 2017-05-10
复星诊断科技(长沙)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the above existing technologies, the detection accuracy needs to be improved, and the detection is not fast enough

Method used

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  • Total cholesterol detection reagent and total cholesterol detection paper
  • Total cholesterol detection reagent and total cholesterol detection paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Step 1: Paste double-sided adhesive tape 40 on the reactive base layer 30 made of PET with a certain hardness, and punch holes for later use.

[0057] Step 2: tear off the double-sided adhesive tape on the reaction base layer 40, and paste the reaction film 50 on the double-sided tape 40 of the PET board. The reaction film 50 needs to completely cover the hole 31 on the PET substrate.

[0058] Step 3. Prepare the detection reagent according to the ratio: 6KU / L cholesterol esterase, 3KU / L cholesterol oxidase, 15KU / L horseradish peroxidase, 3KU / L ascorbate oxidase, 0.20g / L L of 4-aminoantipyrine, 0.20g / L chromogenic substance, 0.15mmol / L (pH 5.5) Tris-HCl buffer, 0.80g / L trehalose, 0.1g / L BSA, 0.15g / L L CHAPS. Add the prepared detection reagent dropwise into the wells of the reaction membrane plate with a dispenser, the weight of each hole is 6mg, and put the membrane plate after dripping into the drying tunnel for drying. The drying temperature is 37°C and the drying ti...

Embodiment 2

[0062] In this example, detection reagent 52 comprises 9KU / L cholesterol esterase, 5KU / L cholesterol oxidase, 20KU / L horseradish peroxidase, 5KU / L ascorbate oxidase, 0.35g / L 4-amino Antipyrine, 0.30g / L chromogenic substance, 0.25mmol / L (pH 5.5) Tris-HCl buffer solution, 1.0g / L trehalose, 0.3g / L BSA, 0.25g / L CHAPS, other With embodiment 1.

Embodiment 3

[0064] In this example, detection reagent 52 includes 6KU / L cholesterol esterase, 5KU / L cholesterol oxidase, 15KU / L horseradish peroxidase, 3KU / L ascorbic acid oxidase, 0.20g / L 4-amino Antipyrine, 0.25g / L chromogenic substance, 0.20mmol / L (pH 6) Tris-HCl buffer, 1.0g / L trehalose, 0.3g / L BSA, 0.25g / L CHAPS, other With embodiment 1.

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Abstract

The invention discloses a total cholesterol detection reagent and total cholesterol detection paper. The total cholesterol detection reagent comprises 6-9 KU / L cholesterol esterase, 3-5 KU / L cholesterol oxidase, 15-20 KU / L horseradish peroxidase, 3-5 KU / L ascorbic acid oxidase, 0.20-0.35 g / L 4-ampyrone, and 0.20-0.30 g / L color-showing matter. The detection reagent contains a plurality of specific enzymes in a specific ratio, and can achieve the purposes of rapid detection and relatively accurate detection. The detection paper comprises a reaction base layer, a reaction layer, a blood filtering layer and a hydrophilic layer, which are overlapped to form a siphon system, thereby further ensuring rapid detection and relatively accurate detection.

Description

technical field [0001] The invention relates to the technical field of total cholesterol detection, in particular to a detection reagent for total cholesterol and a test paper for detection of total cholesterol. Background technique [0002] Currently, methods for detecting the presence or amount of a certain component in blood include liquid reagent testing and dry chemical test strip testing. Dry chemical test paper has the characteristics of convenient use, simple operation and quick results. [0003] According to the flow path of the liquid sample on the dry chemical test paper, the dry chemical test paper can be divided into various types, and the vertical vertical detection test paper is a kind of dry chemical test paper. The vertical vertical detection test paper includes stacking the carriers with the reaction reagents on top of each other, the liquid sample flows through each reaction reagent layer from top to bottom, and finally displays the detection results. Fo...

Claims

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Application Information

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IPC IPC(8): G01N33/92
CPCG01N33/92
Inventor 彭勇李文韬张杰怀
Owner 复星诊断科技(长沙)有限公司
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