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Electrochemical uric acid test strip and manufacturing method thereof

A manufacturing method and a technology of test strips, which are applied in the direction of material electrochemical variables, etc., can solve problems such as interference, and achieve the effects of low cost, easy large-scale production, and simple storage conditions

Active Publication Date: 2014-03-26
SINOCARE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide an electrochemical uric acid test strip that removes the interference of ascorbic acid, so as to solve the interference problem of ascorbic acid in the uric acid test in the prior art

Method used

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  • Electrochemical uric acid test strip and manufacturing method thereof
  • Electrochemical uric acid test strip and manufacturing method thereof
  • Electrochemical uric acid test strip and manufacturing method thereof

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Embodiment Construction

[0031] The embodiments of the present invention will be described in detail below with reference to the accompanying drawings, but the present invention can be implemented in many different ways defined and covered by the claims.

[0032] see figure 1 , figure 2 and image 3 , an electrochemical uric acid test strip, comprising a layer of insulating substrate 1, working electrode 2, counter electrode 3, insulating layer 4, double-sided film 5, hydrophilic film 6 and cover film 7.

[0033] The insulating base 1 is made of stable non-conductive material, preferably PVC or PET material.

[0034]The working electrode 2 and the counter electrode 3 are arranged on the first end of the insulating substrate 1 side by side with a certain distance. The working electrode 2 and the counter electrode 3 are arranged on the insulating substrate 1 by printing. Preferably, the working electrode 2 and the counter electrode 3 are arranged on the insulating substrate 1 by screen printing. A...

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Abstract

The invention provides an electrochemical uric acid test strip comprising an insulating substrate (1), a working electrode (2) and a counter electrode (3), wherein the working electrode (2) and the counter electrode (3) are arranged at a first end of the insulating substrate (1) side by side at an interval; the working electrode (2) is provided with an anti-interference reagent layer; the components of the reagent layer comprise an ascorbic acid oxidase, a macromolecular compound and a buffering solution; and the components of the working electrode (2) comprise a non-aqueous redox electron transfer mediator. The invention further provides a manufacturing method of the electrochemical uric acid test strip. The test strip provided by the invention utilizes the non-water-soluble electron transfer mediator and combines an interference-removing enzyme layer, so that the effect of removing ascorbic acid is obvious. In a test, the interference of the ascorbic acid on uric acid test is very small.

Description

technical field [0001] The invention relates to the field of uric acid testing, in particular to an electrochemical uric acid testing strip. In addition, the present invention also relates to a manufacturing method of the above-mentioned test strip. Background technique [0002] The reported methods for the determination of uric acid mainly include high performance liquid chromatography, electrochemical method, enzymatic method, chemiluminescence method and so on. Among these methods, the direct electrochemical method has the advantages of rapidity, simplicity, and high sensitivity, and the direct electrochemical method does not require the participation of biologically active enzymes in the reaction, and the reaction is not affected by the activity of the enzyme. However, when this method is directly applied to the determination of uric acid, some substances with redox properties will interfere in the test, such as ascorbic acid, dopamine, levodopa, etc., especially the in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
Inventor 彭谢兰
Owner SINOCARE
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