Detection reagent for triglyceride and test paper for triglyceride

A technology for detecting reagents and test strips, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve problems such as insufficient detection speed, and achieve fast and accurate detection. , the effect of rapid detection

Inactive Publication Date: 2017-08-18
复星诊断科技(长沙)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology uses specialized enzyme mixtures that work together with other materials like bone marrow cells or red blood cell membranes for quicker and more precise analysis than previously possible due to their unique properties such as fast response times and high accuracy rates.

Problems solved by technology

This patent describes different ways to measure specific components (such as glucose) present in urine samples without being affected by factors like pH value changes caused by bacterial growth. Current wet chemistry tests require multiple steps involving collecting fluid specimens before analysis, making their process slow and expensive compared to simpler nonwetting type strips that provide rapid response times. Additionally, current solutions involve adding excess material after reactants have been placed, causing unwanted waste materials such as red cell membranes.

Method used

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  • Detection reagent for triglyceride and test paper for triglyceride
  • Detection reagent for triglyceride and test paper for triglyceride
  • Detection reagent for triglyceride and test paper for triglyceride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Step 1: Paste double-sided adhesive tape 40 on the reactive base layer 30 made of PET with a certain hardness, and punch holes for later use.

[0058] Step 2: tear off the double-sided adhesive tape on the reaction base layer 40, and paste the reaction film 50 on the double-sided tape 40 of the PET board. The reaction film 50 needs to completely cover the hole 31 on the PET substrate.

[0059] Step 3. Prepare the detection reagent according to the ratio: 18KU / L lipoprotein lipase, 20KU / L glycerol kinase, 3KU / L glycerol triphosphate oxidase, 7.5KU / L horseradish peroxidase, 3KU / L ascorbate oxidase, 0.20g / L 4-aminoantipyrine, 0.20g / L chromogenic substance, 0.15mmol / L (pH 5.5) Tris-HCl buffer, 0.80g / L seaweed Sugar, 0.1g / L BSA, 0.15g / L CHAPS. Add the prepared detection reagent dropwise into the wells of the reaction membrane plate with a dispenser, the weight of each hole is 6mg, and put the membrane plate after dripping into the drying tunnel for drying. The drying temper...

Embodiment 2

[0063] In this example, the detection reagents include 25KU / L lipoprotein lipase, 30KU / L glycerol kinase, 5KU / L glycerol triphosphate oxidase, 9.0KU / L horseradish peroxidase, 5KU / L ascorbic acid oxidation Enzyme, 0.35g / L 4-aminoantipyrine, 0.30g / L chromogenic substance, 0.25mmol / L (pH 5.5) Tris-HCl buffer, 1.0g / L trehalose, 0.3g / LBSA , 0.25g / L CHAPS, other are all with embodiment 1.

Embodiment 3

[0065] In this example, the detection reagent contains 18KU / L lipoprotein lipase, 30KU / L glycerol kinase, 3KU / L glycerol triphosphate oxidase, 9.0KU / L horseradish peroxidase, 3KU / L ascorbic acid oxidation Enzyme, 0.20g / L 4-aminoantipyrine, 0.25g / L chromogenic substance, 0.20mmol / L (pH 6) Tris-HCl buffer, 1.0g / L trehalose, 0.3g / L BSA, 0.25g / L CHAPS, other are all the same as embodiment 1.

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Abstract

The invention discloses a detection reagent for triglyceride and test paper for the triglyceride. The detection reagent comprises 18-25KU/L lipoprotein lipase, 20-30KU/L glycerol kinase, 3-5KU/L glycerine triphosphate oxidase, 7.5-9.0KU/L horse radish peroxidase, 3-5KU/L ascorbic acid oxidase, 0.20-0.35g/L 4-amino-antipyrineand 0.2-0.30g/L color material. The detection reagent contains multiple specific enzymes for particular proportioning, so that the targets that detection is fast and relatively accurate are achieved. The test paper comprises a reaction base layer, a reaction layer, a blood filtering layer and a hydrophillic layer, and a siphon system is formed by superposing the reaction base layer, the reaction layer, the blood filtering layer and the hydrophilic layer, so that fast detection and relatively accurate detection are further ensured.

Description

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Claims

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Application Information

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Owner 复星诊断科技(长沙)有限公司
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