The invention discloses a preparation method of a dry chemical
reagent for determining the content of beta-
hydroxybutyric acid in
urine. The preparation method comprises the following steps: S1, preparing a buffer
reagent and a protective component; s2, dissolving the biological
enzyme and the chromophoric substrate to form a dissolving
reagent; s3,
coating by using a biological film-forming material to form a
coating reagent; s4, by means of equipment, the
coating reagent is coated and printed in a pore channel of a specified bottom plate base material in a volume quantification mode; the equipment comprises a single-channel type pipettor, a multi-channel type pipettor, a continuous multi-gear
distributor or automatic dot printing equipment. S5, placing the specified bottom plate base material printed with the coating reagent in
forced air drying equipment or
drying equipment with the same efficiency; and S6, measuring the
moisture content after
drying and film forming, and judging whether substances containing biologically active enzymes and substrates in the reagent are completely locked to the position of the net-shaped cavernous body structure of the biological film forming material or not based on the
moisture content. The invention further discloses application of the dry chemical reagent for determining the content of beta-
hydroxybutyric acid in
urine, and the dry chemical reagent is applied to a sample formed by
urine.