Dry chemistry hydrogen peroxide detecting method based on heme hexapeptide

Abstract

The invention discloses a dry chemistry hydrogen peroxide detecting method based on heme hexapeptide, and belongs to the technical field of the biochemical detection. The method comprises the following steps: spraying a heme hexapeptide mixture reagent layer on a baseplate polyester film of a detecting card, spraying a titanium dioxide diffusion layer on the reagent layer, wherein the reagent layer is a mixture of a polymer microsphere, the heme hexapeptide, 4-AAP-chlorophenol, phosphatebuffer solution, a surfactant and a water-soluble binder. The beneficial effect is that the dry chemistry H2O2 detecting method is provided by using a principle that DhHP-6 has a peroxidase function, and a quantity of H2O2 in a liquid sample to be detected is calculated through detecting a change of diffusereflection light after a reaction; the method has the characteristics of a rapid response rate, high sensitivity, a wide linearity range, a small system volume, easy carrying, and no external reagent; and the method can be extensively applied to the field of monitoring the H2O2 quantity in the liquid sample by medicine health and food production enterprises, agricultural trade markets, quality supervision and environment monitoring departments and the like.

Description

technical field [0001] The invention belongs to the technical field of biochemical detection. Background technique [0002] Hydrogen peroxide (H 2 O 2 ) is a commonly used oxidant and plays an important role in food production, biomedicine, pharmaceuticals, industry and the environment. But a certain concentration of H 2 O 2 After contact with human skin or accidental ingestion, it will cause physical discomfort or endanger physical health, and in severe cases, it will induce a variety of diseases. The national food safety management department and environmental protection department have 2 O 2 There are strict requirements for residues in food, content in rivers and lakes, and residues after industrial sewage treatment, but there are still some unscrupulous enterprises and individuals who ignore national laws and regulations and abuse H. 2 O 2 , so in order to monitor H more effectively 2 O 2 use to establish a fast, accurate, and economical determination of H 2 ...

AI Technical Summary

Problems solved by technology

National food safety management department and environmental protection department 2 o 2 There are strict requirements for the residues in food, the content in rivers and lakes, and the residues after industrial sewage treatment, but there are still some unscrupulous companies and individuals who ignore the laws and regulations of the country and abuse H 2 o 2 , so in order to monitor H more effectively 2 o 2 The use of the establishment of fast, accurate and economical determination of H 2 o 2 The method of concentration has important implications

[0003] h 2 o 2 Concentration detection methods include titration, spectrometry, chemical hair assay, fluorescence assay, liquid chromatography and electrochemical methods, etc., these detection methods are based on the catalysis of peroxidase (Peroxidase, POD) to Although POD has high specificity and sensitivity, the application of free natural enzymes is limited by their poor stability and high cost

And the above methods all need relatively large-scale instrument and carry out measurement in the laboratory, are not suitable for carrying out on-the-spot instant inspection

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