Dry chemical method TORCH detection reagent kit and method of manufacture

A technology for detection kits and manufacturing methods, applied to the field of rapid detection of TORCH virus antibodies in human serum/plasma/whole blood

Active Publication Date: 2008-07-09
WUXI SHENRUI BIO PHARMA
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Problems solved by technology

The common disadvantage of these detection methods is that they all require professional supporting instruments or professional technicians to operate.

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  • Dry chemical method TORCH detection reagent kit and method of manufacture

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Embodiment Construction

[0041] A kind of dry chemical method TORCH rapid detection kit of the present invention, in the detection unit of kit, comprise the matrix lath that is used to support reaction system, the nitrocellulose membrane that is used for adsorption reactant (or as PVDF etc. is used for adsorption protein membrane material), reactant (including diluent: composed of buffer (pH6.0-8.2), protein stabilizer and 0.1% sodium azide; enhancement solution: containing sodium chloride and 0.1% azide Sodium; binding solution: composed of alkaline phosphatase combined with goat anti-human antibody (or other animal anti-human antibody), protein stabilizer and 0.1% sodium azide dissolved in buffer (pH6.0-8.8) ; Development solution: by dissolving the chromogenic substrate (can be 5-bromo-4-chloro-3-indolyl phosphate, p-nitro blue tetrazolium chloride NBT) dissolved in the buffer solution (pH8.8~11.0) Or TMB, OPD, etc. and 0.1% sodium azide). The whole kit also includes a disposable tube for the reage...

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Abstract

The invention relates to a TORCH detection kit using a dry chemistry method and a preparation method of the kit. The detection kit comprises a detection unit consisting of a PVC strip for supporting a reaction system, a cellulose nitrate membrane reaction solution system for adsorbing reactants, a protein stabilizer and sodium azide; an enhancement solution containing sodium chloride and sodium azide; a binding solution containing alkaline phosphatase dissolved in a buffer solution and combined with goat anti human body, a protein stabilizer and sodium azide; and a developing solution containing a developing substrate dissolved in a buffer solution. The entire kit also includes a disposable tube for allowing the test strip to react therein and an anti-moisture drying agent. The kit of the invention has high detection speed, low detection cost, and no need for any auxiliary equipment as well as a great amount of expensive detection equipment in the detection process. The kit helps to develop rapid TORCH screening in various occasions in clinic units and family planning systems of various levels, thus promoting national health.

Description

technical field [0001] The invention relates to a kit for rapidly detecting whether human serum / plasma / whole blood contains TORCH virus antibody and a manufacturing method of the kit. Background technique [0002] TORCH refers to the abbreviation of the English names of the four pathogens: toxoplasma gondii, rubella virus, cytomegalovirus, and herpes simplex virus. It can be seen from the title of the article in the reference that this is a common name. They are viral infections in children. The main pathogenic microorganisms of viral infections. TORCH detection is of great significance. When it is used to judge the type of clinical infection in children, it can be used to know the clinical choice of drug regimen according to the degree of virus infection; when it is used to judge the infection of pregnant women, it can reduce the incidence of birth defects. [0003] The methods currently used for the detection of TORCH include: immunofluorescence, complement fixation, pass...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/544G01N33/531
Inventor 盛青松
Owner WUXI SHENRUI BIO PHARMA
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