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Magnetic bead reagent for chemiluminescence immunoassay reagent

A technology of chemiluminescent immunity and detection reagents, which is applied in the field of medicine and can solve the problems of precipitation container surface and adhesion

Inactive Publication Date: 2018-06-29
NINGBO ZIYUAN MEDICAL DEVICES INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the density of immunomagnetic beads is much higher than that of water, precipitation and surface adhesion of the container are often prone to occur in aqueous phase storage reagents

Method used

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  • Magnetic bead reagent for chemiluminescence immunoassay reagent
  • Magnetic bead reagent for chemiluminescence immunoassay reagent
  • Magnetic bead reagent for chemiluminescence immunoassay reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] ——Preparation of magnetic bead reagent

[0055]

Embodiment 2

[0057] ——Composite formulation of different thickeners

[0058] According to the preparation method of the magnetic bead reagent in Example 1, the combinations and dosages of different thickeners (see Table 1).

[0059] Table 1 Combination and dosage of different thickeners in the magnetic bead reagent

[0060]

[0061]

Embodiment 3

[0063] ——The temperature dependence of the corresponding physical form of different formulations of magnetic bead reagents under different temperature conditions.

[0064] Prepare the magnetic bead reagent according to Examples 1-2, and identify the temperature dependence of the physical form of the magnetic bead reagent in different combinations (Table 2).

[0065] Table 2 The morphology of magnetic bead reagents at different temperatures

[0066] group

[0067] Conclusion: According to the results in Table 2, the temperature dependence of the physical form of the magnetic bead reagent of the present invention is met, that is, it is in the form of jelly or solid at 25°C or less (especially 2-8°C).

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Abstract

The invention relates to a magnetic bead reagent for a chemiluminescence immunoassay reagent. The magnetic bead reagent is prepared from immunomagnetic beads, a basic solution and a thickening agent.The physical form of the reagent is temperature-dependent, that is, the reagent is colloidal or solid under a certain temperature condition. The fluidity of the reagent is extremely low, the physicalform of the reagent is liquid as the temperature rises, the fluidity is restored, and the immunomagnetic beads are uniformly dispersed in the reagent and are not prone to settling. When the reagent isin a colloidal or solid form, the loss caused by adhesion of the immunomagnetic beads to packaging films or tube walls in the cold chain transportation process can be effectively avoided, and accordingly, detection accuracy is guaranteed; when the physical form is not liquid due to temperature change, the immunomagnetic beads are uniformly dispersed in the reagent and are not prone to settling. Consistency of the magnetic bead component reagent can be ensured during subpackaging. The magnetic bead reagent is suitable for luminescent immunoassay kits, especially kits or reagent strips for POCT(point-of-care testing).

Description

technical field [0001] The invention relates to a magnetic bead reagent used for chemiluminescent immunoassay reagents, which is suitable for chemiluminescent immunoassay reagents and belongs to the technical field of medicine. Background technique [0002] Immunomagnetic beads are usually made of small metal particles (Fe 2 o 3 or Fe 3 o 4 ) core and polymer materials containing specific chemical groups. Immunomagnetic beads have superparamagnetism and can be evenly dispersed in the solution. The functional groups on the surface such as amino, carboxyl, sulfhydryl, etc. can be covalently or non-covalently coupled with antibodies, and then bind to the corresponding antigen or antibody. Under the action of a magnetic field, it can be quickly adsorbed to achieve the purpose of purifying genes, proteins, cells, microorganisms, etc. 1374-1385). In addition, immunomagnetic beads have good surface effect, large specific surface area, large functional groups and selective ads...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/76
CPCG01N21/76G01N33/54326
Inventor 蔡怡程策李锐
Owner NINGBO ZIYUAN MEDICAL DEVICES INC
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