Dry chemistry test paper for quantitative determination of urea content in human blood

Abstract

The invention belongs to the technical field of in-vitro clinical diagnostic reagent, in particular to a dry chemistry test paper for quantitative determination of urea content in human blood. The test paper comprises an upper, a middle and a lower support layers and testing layers thereof and is separated into a hand-held area and a test area. The test area is composed of a diffusion layer, a reagent layer and a color reagent layer from the top down. A loading hole is arranged on the upper support layer, a test sample is introduced into the diffusion layer from the loading hole to penetrate into the reagent layer, then enzymatic reaction is carried out on the reagent layer, ammonia is generated by catalyzing urea by using urease, and under the alkaline environment, the generated ammonia spreads to the color reagent layer to perform color reaction with the indicator of ammonia. The variation of optical density can be measured with a reflectance spectrophotometer through a test hole onthe lower support layer. The test paper can be used to quantitatively test the urea content in human blood, provides basis for the diagnosis of renal function, and is suitable to be used in emergencyrooms of the hospitals.

Description

technical field [0001] The invention belongs to the technical field of in vitro clinical diagnostic reagents, in particular to a dry chemical test paper suitable for quantitatively measuring urea content in human blood. Background technique [0002] Urea is the end product of protein metabolism in the human body. Amino acids in the body are decomposed into α-keto acids and NH by deamination 3 , NH 3 Enter the urea cycle and CO in hepatocytes 2 This produces urea. The liver is the most important organ that produces urea. The amount of urea produced depends on dietary protein intake, tissue protein catabolism, and liver function. The urea produced in the liver is mainly excreted by the kidneys, and a small part is excreted by sweat. It is mainly filtered by the glomeruli and excreted with the urine. If kidney function is impaired, the glomerular filtration rate decreases and the concentration of urea in the blood increases. [0003] The urea content in blood is one of t...

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Problems solved by technology

[0009] Some of the above products have complex production processes, such as Roche test strips; some require specialized equipment and technology, such as Johnson & Johnson and Fuji dry tablets

In particular, its reagents are expensive and can only be used in large hospitals, and are difficult to be accepted by small and medium hospitals, especially small hospitals, hindering the general application of dry chemical products

Moreover, the dry biochemical analyzers used in conjunction with it are mostly large and medium-sized instruments, which have certain limitations in terms of price and portability.

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