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Dry chemical test paper for quantitatively determining human alanine aminotransferase

A technology for the quantitative determination of alanine amino groups, which is applied in the field of in vitro clinical diagnostic reagents, can solve problems such as function and speed limitations, and achieve the effects of good uniformity, simple manufacturing process, and high color intensity

Inactive Publication Date: 2008-01-16
SHANGHAI KEHUA BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When measuring a single alanine aminotransferase item, such as mobile blood collection vehicles, its function and speed will be limited

Method used

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  • Dry chemical test paper for quantitatively determining human alanine aminotransferase

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: The upper and lower support layers are made of PVC sheets, and the sample hole 3 and the test hole 4 are punched out with a punching machine. The diameter of the holes is 4mm, and the hole distance is 8mm. The substrate layer 5 adopts glass fiber and is pasted on the upper supporting layer. The color-developing layer 6 adopts a nylon film, which is pasted on the lower support layer. The prepared substrate solution and chromogenic solution were spotted on the substrate layer 5 and the chromogenic layer 6 respectively with a BIO-DOT dot sprayer, 6 μl per well. Put the sprayed test paper into a vacuum drying oven at 30-50°C, and take it out after drying. The upper and lower support layers are pasted together, and the sample hole 3 is aligned with the test hole 4. Cut the test paper into 8mm wide test strips with a hob machine.

[0020] The reagent composition is as follows:

[0021] Phosphate buffer: 50mM

[0022] L-alanine: 500mM

[0023] Sodium α-keto...

Embodiment 2

[0036]Embodiment 2: The upper and lower support layers are made of PET sheets, and the sample hole 3 and the test hole 4 are punched out with a punching machine. The diameter of the holes is 4 mm, and the hole distance is 8 mm. The substrate layer 5 is made of glass fiber, and the color-developing layer 6 is made of PES film with a width of 8 mm. Soak the substrate layer 5 and the color developing layer 6 in the substrate solution and the color developing solution respectively, take them out after completely soaking, and scrape them flat. Put the soaked test paper into a vacuum drying oven at 30-50°C, and take it out after drying. The substrate layer 5 is pasted on the upper supporting layer, and the color-developing layer 6 is pasted on the lower supporting layer. The upper and lower support layers are pasted together, and the sample hole 3 is aligned with the test hole 4. Cut the test paper into 8mm wide test strips with a hob machine.

[0037] The reagent composition is ...

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Abstract

The invention belongs to the in vitro clinical diagnosis reagent field and specifically relates to a dry chemistry test paper which quantitatively determines the activity of alanine aminotransferase in human body; the dry chemistry test paper consists of an upper and a lower supporting layers in long-belt-shapes; a loading hole, a substrate layer, a visualization layer and a test hole are provided in a test area from top to bottom; the substrate layer can adopt the materials such as glass fibre, filter paper and non-woven fabrics and is soaked with reagents such as a substrate; the visualization layer is composed of various filter papers, non-woven fabrics or synthetic film and is soaked with reagents such as chromogen. A sample penetrates into the substrate layer through the loading hole and flows to a sample surface of the visualization layer; a liquid with detected ingredients penetrates to a detecting surface; the color of chromogen is changed under chemical reaction; the liquid penetrates through the test hole on the lower supporting layer and reaches the detecting surface of the visualization layer; the photodensity of the liquid can be determined on the detecting surface. The test paper is used to rapidly test the activity of alanine aminotransferase in whole blood, serum or plasma in the human body, which is one of important indexes of the detection of liver function.

Description

technical field [0001] The invention belongs to the technical field of in vitro clinical diagnostic reagents, in particular to a dry chemical test paper suitable for measuring biochemical indicators such as alanine aminotransferase and aspartate aminotransferase in human whole blood, serum or plasma. technical background [0002] Alanine aminotransferase, also known as alanine aminotransferase, catalyzes the transfer of amino groups between L-alanine and L-glutamic acid. Alanine aminotransferase mainly exists in the cytoplasm of liver cells, and its content is lower in cardiac muscle, skeletal muscle and other tissues. Alanine aminotransferase is a liver-specific enzyme. Elevated serum values ​​indicate leakage and degeneration of the plasma membrane of hepatocytes. The degree of increase is related to the number of affected cells. [0003] The reagents for clinical determination of alanine aminotransferase can be divided into liquid reagents and dry chemical test papers. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N21/78C12Q1/52
Inventor 田漪王缦朱世成
Owner SHANGHAI KEHUA BIO ENG
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