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Detection reagent and test paper for uric acid

A technology for detecting reagents and test papers, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions. It can solve the problems of high material requirements, complex processes, and complex test paper production processes. problem, to achieve the effect of strong anti-interference ability and eliminate interference

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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this kind of liquid-locking structure has a complicated process, requiring the liquid-locking parts to absorb liquid at a rate less than or equal to the liquid-absorbing speed of the reaction layer. The material requirements for the liquid-locking parts are relatively high, and a process is added to the test paper production process, making the production of test paper The process is more complicated
[0006] In addition, in the above existing technologies, the detection accuracy needs to be improved, and the detection is not fast enough

Method used

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  • Detection reagent and test paper for uric acid
  • Detection reagent and test paper for uric acid
  • Detection reagent and test paper for uric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A detection test paper 1 for uric acid, its manufacture method comprises the following steps:

[0058] Step 1: Paste double-sided adhesive tape 200 on the reactive base layer 100 made of PET material with a certain hardness, and punch through holes 110 for later use.

[0059] Step 2. Tear off the oil paper of the double-sided adhesive 200 on the reaction base layer 100, and paste the film material 320 on the double-sided adhesive tape 200 of the PET board. The film material 320 needs to completely cover the through hole 110 on the PET substrate.

[0060] Step 3, first prepare detection reagent 310: 12KU / L of uric acid oxidase, 10KU / L of horseradish peroxidase, 15KU / L of ascorbate oxidase, 0.20g / L of 4-aminoantipyrine , 0.15mmol / L PBS buffer solution, 1.0g / L trehalose, 0.35g / L BSA and 0.30g / L chromogenic substance. Add the prepared detection reagent 310 dropwise to the corresponding through hole 110 of the membrane material 320 with a dispenser. After 20 minutes, the r...

Embodiment 2

[0064] In the preparation method of the uric acid detection test paper 1 of this embodiment, the detection reagent 310 includes 14KU / L uric acid oxidase, 9KU / L horseradish peroxidase, 16KU / L ascorbate oxidase, 0.25g / L 4-aminoantipyrine, 0.18mmol / L PBS buffer, 1.1g / L trehalose, 0.33g / L BSA and 0.28g / L chromogenic substance. Other steps are all the same as in Example 1.

Embodiment 3

[0066] In the preparation method of the uric acid detection test paper 1 of this embodiment, the detection reagent 310 includes 15KU / L uric acid oxidase, 8KU / L horseradish peroxidase, 18KU / L ascorbate oxidase, 0.28g / L 4-aminoantipyrine, 0.19mmol / L PBS buffer, 1.2g / L trehalose, 0.30g / L BSA and 0.25g / L chromogenic substance. Other steps are all the same as in Example 1.

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Abstract

The invention discloses a detection reagent and test paper for uric acid. The detection reagent comprises 12 to 18 KU / L of urate oxidase, 5 to 10 KU / L of horseradish peroxidase, 15 to 20 KU / L of ascorbic acid oxidase, 0.20 to 0.35 g / L of 4-aminoantipyrine and 0.20 to 0.30 g / L of a chromogenic substance. The detection reagent provided by the invention contains a plurality of specific enzymes in a specific ratio and can achieve the purpose of rapid and accurate detection of the content of uric acid. The test paper for uric acid comprises a reaction substrate layer, a reaction layer, a hemofiltration layer and a sample suction layer. The above-mentioned layers are superposed to form a siphon system, so the test paper has better anti-interference capability, effectively eliminates interference by endogenous substances and further guarantees rapid and accurate detection of the content of uric acid.

Description

technical field [0001] The invention relates to the technical field of uric acid detection, in particular to a uric acid detection reagent and a uric acid detection test paper. Background technique [0002] Currently, methods for detecting the presence or amount of a certain component in blood include liquid reagent testing and dry chemical test strip testing. Dry chemical test paper has the characteristics of convenient use, simple operation and quick results. [0003] According to the flow path of the liquid sample on the dry chemical test paper, the dry chemical test paper can be divided into various types, and the vertical vertical detection test paper is a kind of dry chemical test paper. The vertical vertical detection test paper includes stacking the carriers with the reaction reagents on top of each other, the liquid sample flows through each reaction reagent layer from top to bottom, and finally displays the detection results. For example, U.S. Patent No. 4,816,22...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 彭勇李文韬李小慧
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