Glycosylated serum protein detection reagent and application thereof

A glycated serum protein and detection reagent technology, applied in the field of medical testing, can solve the problems of poor accuracy, low anti-interference ability, low repeatability, etc., and achieve the effects of ensuring accuracy, high precision of test results, and strong bactericidal performance.

Active Publication Date: 2016-01-06
郑州金域临床检验中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the defects of poor detection accuracy, low repeatability and low anti-interference ability in the glycosylated serum protein detection reagent in the prior art, the purpose of the present invention is to provide a glycosylated serum protein detection reagent and its application to solve the above problems

Method used

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  • Glycosylated serum protein detection reagent and application thereof

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Effect test

Embodiment 1

[0028] Embodiment 1, glycosylated serum protein detection reagent

[0029] The glycosylated serum protein detection reagent includes reagent R1 and reagent R2 with a volume ratio of 3:1, and the reagent R1 includes the following components:

[0030] Potassium phosphate buffer with a pH value of 6.5 10mmol / L, uricase 2U / ml, ascorbate oxidase 8U / ml, alkanolamide polyoxyethylene ether 20mmol / L, 1,2-propanediol 5mg / ml, calcium chloride 25μmol / L and paraben 0.1g / L;

[0031] The reagent R2 comprises the following components:

[0032] MES buffer with a pH value of 9.5 0.1mmol / L, alkyl polyglucoside 30mmol / L, 2-(4-iodobenzene)-3-(2,4-dinitrobenzene 1)-5-(2, 4-disulfobenzene 1)-2H-tetrazolium 0.1mmol / L and paraben 0.1g / L.

Embodiment 2

[0033] Embodiment 2, glycosylated serum protein detection reagent

[0034] The glycosylated serum protein detection reagent includes reagent R1 and reagent R2 with a volume ratio of 3:1, and the reagent R1 includes the following components:

[0035] Potassium phosphate buffer with a pH value of 7.0 20mmol / L, uricase 6U / ml, ascorbate oxidase 12U / ml, alkanolamide polyoxyethylene ether 40mmol / L, ethylene glycol 6mg / ml, calcium chloride 35μmol / L and paraben 0.2g / L;

[0036] The reagent R2 comprises the following components:

[0037] MES buffer with a pH value of 10.5 1mmol / L, alkyl polyglucoside 45mmol / L, 2-(4-iodobenzene)-3-(2,4-dinitrobenzene 1)-5-(2,4 -Disulfobenzene 1)-2H-tetrazolium 0.5mmol / L and paraben 0.2g / L.

Embodiment 3

[0038] Embodiment 3, glycosylated serum protein detection reagent

[0039] The glycosylated serum protein detection reagent includes reagent R1 and reagent R2 with a volume ratio of 3:1, and the reagent R1 includes the following components:

[0040]Potassium phosphate buffer with a pH value of 7.5 30mmol / L, uricase 8U / ml, ascorbate oxidase 12U / ml, alkanolamide polyoxyethylene ether 60mmol / L, ethylene glycol 10mg / ml, calcium chloride 40μmol / L and paraben 0.4g / L;

[0041] The reagent R2 comprises the following components:

[0042] MES buffer solution with a pH value of 10.8 1.5mmol / L, alkyl polyglucoside 80mmol / L, 2-(4-iodobenzene)-3-(2,4-dinitrobenzene 1)-5-(2, 4-disulfobenzene 1)-2H-tetrazolium 1.2mmol / L and paraben 0.4g / L.

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Abstract

Belonging to the technical field of medical examination, the invention in particular relates to a glycosylated serum protein detection reagent and application thereof. The reagent comprises a reagent R1 and a reagent R2 in a volume ratio of 3:1, the reagent R1 is mainly composed of a potassium phosphate buffer solution, uricase, ascorbic acid oxidase, alkanolamide polyoxyethylene ether, polyol, calcium chloride and a preservative, and the reagent R2 is mainly composed of an MES buffer solution, alkyl polyglucoside, 2-(4-iodobenzene)-3-(2, 4-dinitrobenzene1)-5-(2, 4-di-sulfobenzene1)-2H-tetrazole and a preservative. The glycosylated serum protein detection reagent provided by the invention has the advantages of stronger anti-interference ability, high determination sensitivity and precision, and good repeatability of determination result, also the detection reagent has high stability and good water solubility, and is conducive to clinical application of the glycosylated serum protein detection reagent.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a detection reagent for glycosylated serum protein and an application thereof. Background technique [0002] Glycosylated serum protein (Glycosylated serum protein, GSP) is the product of the slow non-enzymatic glycation reaction of various proteins in serum with glucose. Determination of the concentration of glycosylated serum protein can reflect the average blood sugar level of the human body in the previous 2-3 weeks, and is not affected by the blood sugar concentration at that time. It is a good indicator for blood sugar monitoring of diabetic patients. Therefore, the determination of glycosylated serum protein is of great significance in the diagnosis, short-term monitoring and efficacy evaluation of diabetes. [0003] The detection methods of glycosylated serum protein initially used radiolabeling technology, perthiobarbituric acid method, affinity chro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 燕启江郭周萍庞柳刘菲菲
Owner 郑州金域临床检验中心有限公司
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