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Test paper for measuring high density lipoprotein cholesterol and preparation method and application thereof

A high-density lipoprotein and cholesterol technology, which is applied in the field of test paper for measuring high-density lipoprotein cholesterol and its preparation, to achieve the effects of improving stability, simple structure, and eliminating interference

Inactive Publication Date: 2019-06-14
WUHAN J H BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to suppress the reaction produced by lipoproteins other than HDL, these two methods of directly measuring HDL cholesterol must use expensive reagents such as PEG-modified enzymes or antibodies, and also require large-scale equipment for HDL cholesterol detection.

Method used

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  • Test paper for measuring high density lipoprotein cholesterol and preparation method and application thereof
  • Test paper for measuring high density lipoprotein cholesterol and preparation method and application thereof
  • Test paper for measuring high density lipoprotein cholesterol and preparation method and application thereof

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preparation example Construction

[0036] Embodiments of the present invention also provide a method for preparing a test paper for measuring high-density lipoprotein cholesterol, comprising the steps of:

[0037] (1) After mixing buffer salt, divalent metal ions, dextran sulfate sodium salt, hemagglutinin or Anti-RBC antibody evenly, prepare red blood cell separation solution; after mixing buffer salt, surfactant and polyol , prepared as auxiliary separation liquid; buffer salt, surfactant, polysaccharide, cholesterol esterase, cholesterol oxidase, peroxidase, ascorbic acid oxidase, oxidizing substance and chromogenic agent were mixed uniformly to prepare reaction liquid;

[0038] (2) After soaking the separation material in the erythrocyte separation solution, the separation membrane in the auxiliary separation solution and the reaction membrane in the reaction solution, dry to obtain the erythrocyte separation layer, the auxiliary separation layer and the reaction layer in sequence, and then combine with Oth...

Embodiment 1

[0046] Embodiment 1 of the present invention provides a kind of test paper for measuring high-density lipoprotein cholesterol, and it comprises upper plate, blood diffusion layer, erythrocyte separation layer, auxiliary separation layer, reaction layer and lower plate composition, wherein:

[0047] The upper plate is made of fixed-size ABS plastic, and a sample slot is opened in the middle of the upper plate;

[0048] The blood diffusion layer is made of commercially available hydrophilic gauze (the hydrophilic series gauze of SEFAR can be used), and the gauze is cut into a size of 4mm*5mm for later use;

[0049] The erythrocyte separation layer is made of glass fiber material (commercially available PALL series glass fiber material can be used) impregnated in the erythrocyte separation liquid, and the impregnation capacity is 460g / m 2 (that is, impregnate 460g of red blood cell separation solution per cubic meter of glass fiber material), put it in a blast drying oven for dry...

Embodiment 2

[0063] Embodiment 2 of the present invention provides a kind of test paper for measuring high-density lipoprotein cholesterol, and it comprises upper plate, blood diffusion layer, erythrocyte separation layer, auxiliary separation layer, reaction layer and lower plate composition, wherein:

[0064] The upper plate is made of fixed-size ABS plastic, and a sample slot is opened in the middle of the upper plate;

[0065] The blood diffusion layer is made of commercially available hydrophilic gauze (the hydrophilic series gauze of SEFAR can be used), and the gauze is cut into a size of 4mm*5mm for later use;

[0066] The red blood cell separation layer is made of glass fiber material (commercially available PALL series glass fiber material can be used) impregnated in the red blood cell separation liquid, and the impregnation capacity is 450g / m 2 , put it into a blast drying oven for drying, the drying temperature is 50°C, and the drying time is 24 hours. After drying, the glass fi...

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Abstract

The invention discloses test paper for measuring high density lipoprotein cholesterol and a preparation method and application thereof. The test paper comprises a red blood cell separation layer, an auxiliary separation layer and a reaction layer, wherein the red blood cell separation layer, the auxiliary separation layer and the reaction layer are prepared from a separation material, a separationmembrane and a reaction membrane through carrying out steeping by red blood cell separation solution, auxiliary separation solution and reaction solution and then carrying out drying; the red blood cell separation solution comprises the following components: buffer salt, a divalent metal ion, dextran sulfate sodium salt with a molecular weight of 30000-500000, hemagglutinin or an Anti-RBC antibody; the auxiliary separation solution comprises the following components: buffer salt, a surfactant I and polyhydric alcohol; and the reaction solution comprises the following components: buffer salt,a surfactant II, polysaccharide, cholesterol esterase, cholesterol oxidase, peroxidase, ascorbic acid oxidase, an oxidizing substance and a color developing agent. The test paper is capable of simplyand rapidly detecting the contents of high density lipoprotein cholesterol. The invention furthermore provides a preparation method and application of the test paper.

Description

technical field [0001] The invention relates to the technical field of measuring cholesterol, in particular to a test paper for measuring high-density lipoprotein cholesterol and a preparation method and application thereof. Background technique [0002] In addition to free cholesterol, most of the cholesterol in human blood is combined with lipoproteins such as very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) to form lipoprotein cholesterol. Among them, HDL, also known as a1 lipoprotein, is relatively rich in phospholipids, and its content in serum is about 300 mg / dl. The protein part, A-I is about 75%, and A-II is about 20%. HDL receives cholesterol from various tissues including the arterial wall, transports it to the liver through direct reverse transport or indirect reverse transport, and then clears it out of the blood. Therefore, HDL is involved in the removal of cholesterol accumulated in cells. Therefore, HDL c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 王健斌龚贻洲李强许俊峰
Owner WUHAN J H BIO TECH
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