Working electrode biological reactant and electrode type test strip

A working electrode and biological reaction technology, applied in the field of electrochemical detection, can solve the problems of failing to significantly improve the anti-interference ability of the test strip, reducing the precision of the test strip, and current response signal interference.

Inactive Publication Date: 2016-05-11
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the experimental results show that the addition of the traditional ascorbate oxidase to the enzyme to be tested fails to significantly improve the anti-interference ability of the test strip ascorbic acid. At the same time, adding the traditional ascorbate oxidase will produce some unknown chemical reactions. The current response signal of its own enzyme reaction produces serious interference (such as reducing the precision of the test strip, reducing the sensitivity of the current response, and reducing the linear range)
Therefore, at present, the method of directly mixing the enzyme to be tested with the traditional ascorbate oxidase to reduce the interference of ascorbic acid is rarely used in disposable commercial disposable electrochemical test strips

Method used

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  • Working electrode biological reactant and electrode type test strip
  • Working electrode biological reactant and electrode type test strip
  • Working electrode biological reactant and electrode type test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The structure, composition and performance test of embodiment 1 electrochemical test strip

[0066] The schematic diagram of the electrode type test strip is shown in figure 1 As shown, the schematic diagram of its structural decomposition is shown in figure 2 shown.

[0067] figure 1 Schematic diagram of the structure of an electrochemical test strip used for the glucose electrochemical sensor. The test strip includes a board body 100 and electrodes located inside the board body 100 , and the two ends of the board body 100 are collecting terminals 20 and electrical contact terminals 10 respectively. Wherein, the collection end 20 is used to receive fluid samples, and the inside of the collection end 20 is provided with a liquid chamber as a liquid sample chamber. The end faces of the collecting end 20) communicate with the liquid chamber respectively, and the bottom surface of the collecting end 20 is provided with a slot 34 communicating with the liquid chamber. ...

Embodiment 2

[0097] Anti-interference test of embodiment 2 glucose electrochemical sensor

[0098] This embodiment is to reduce the interference of ascorbic acid on the glucose electrochemical sensor with oxidation current as the detection signal. The structure of the electrochemical test strip used in this embodiment is the same as that in Embodiment 1.

[0099] (1) Single working electrode mode

[0100] When the single working electrode mode is adopted, the electrode 12 is the working electrode, and the electrodes 11 and 13 are reference electrodes. The enzyme membrane (biological reaction membrane) component on electrode 12 surface is identical with the enzyme membrane component in embodiment 1, coats A, B and C three kinds of enzyme membrane components respectively, specifically as shown in table 2 in embodiment 1 . Electrodes 11 and 13 are coated with oxidized electron mediator potassium ferricyanide, polymer adhesion promoter hydroxyethyl cellulose and filler lactose, which are th...

Embodiment 3

[0122] Embodiment 3 Anti-interference test of blood ketone electrochemical sensor

[0123] This embodiment is to reduce the interference of ascorbic acid on the blood ketone electrochemical sensor which uses the oxidation current as the detection signal. The structure of the electrochemical test strip adopted in this embodiment is the same as that of Embodiment 1.

[0124] (1) Single working electrode mode

[0125] When the single working electrode mode is adopted, the electrode 12 is the working electrode, and the electrodes 11 and 13 are reference electrodes. The enzyme film (biological reaction film) component on the surface of the working electrode 12 is divided into the following three situations:

[0126] A: coated with β-hydroxybutyrate dehydrogenase, nicotinamide adenine dinucleotide (NAD + ), new ascorbate oxidase (N-ASO, 216U / mg, purchased from Amano, Japan), oxidized electron mediator Azure I, macromolecule adhesion promoter hydroxyethylcellulose and filler lacto...

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Abstract

The invention relates to the technical field of electromechanical detection, in particular to a working electrode biological reactant and an electrode type test strip. The working electrode biological reactant comprises ascorbic acid oxidase of microorganism origin, a biological enzyme corresponding to a substance to be detected, and an electronic mediator. In the invention, interference of ascorbic acid is reduced by means of directly mixing a target analyte special enzyme and novel ascorbic acid oxidase (N-ASO), with no interference (for example, no influence on precision of the test strip and no reduction in the current response sensitivity) on enzyme reaction current response signals of the target analyte special enzyme; an electrochemical detection method of the invention is applicable to an electrochemical test strip using single or multiple working electrodes, enabling a reduction in the interference of the ascorbic acid upon the electromechanical test strip using oxidized current as a detection signal and a reduction in the interference of the ascorbic acid upon the electrochemical test strip using reduction electric current as a detection signal.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, in particular to a working electrode biological reactant and an electrode type test strip. Background technique [0002] POCT, point-of-care testing, refers to clinical testing performed next to the patient, usually not necessarily performed by clinical examiners. In most cases, POCT is a new type of method that analyzes immediately at the sampling site, saves the complicated processing procedures of specimens in the laboratory, and quickly obtains test results. Now POCT has become an important part of laboratory medicine, and is favored by people because of its miniaturized experimental equipment, simple operation, and instant report results. [0003] Electrochemical test strips based on the principle of enzymatic amperometric biosensors are widely used in various point-of-care testing (POCT) products, such as commercialized electrochemical test strips for blood glucose, blood...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/416
CPCG01N27/3272G01N27/3277G01N27/416
Inventor 黄钊蔡晓华
Owner SINOCARE
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