Detection kit for LDL (low-density lipoprotein) cholesterol and use method of detection kit

A low-density lipoprotein and detection kit technology, which is applied in the field of kits for measuring chemical and physical properties of materials, achieves good stability and low toxicity, good compatibility, and maintains stability.

Active Publication Date: 2017-12-22
WHITMAN BIOTECH NANJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method described in this document has difficulties in solving the problems that it requires incubation for 15 minutes or longer, and that it requires two types of reagents

Method used

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  • Detection kit for LDL (low-density lipoprotein) cholesterol and use method of detection kit
  • Detection kit for LDL (low-density lipoprotein) cholesterol and use method of detection kit
  • Detection kit for LDL (low-density lipoprotein) cholesterol and use method of detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The low-density lipoprotein cholesterol detection kit raw material and proportioning of the present embodiment are as follows:

[0045] The kit consists of reagent 1 and reagent 2;

[0046] Reagent 1 is as follows:

[0047]

[0048] All the other are GOOD'S buffer solution MOPS (3-(N-morpholine) propanesulfonic acid) (MOPS is the main component of buffer solution), and described buffer solution pH value is 7.0;

[0049] Reagent 2 is as follows:

[0050]

[0051] The rest is GOOD'S buffer MOPS, and the buffer pH value is 7.0.

Embodiment 2

[0053] The low-density lipoprotein cholesterol detection kit raw material and proportioning of the present embodiment are as follows:

[0054] The kit consists of reagent 1 and reagent 2;

[0055] Reagent 1 is as follows:

[0056]

[0057] All the other are GOOD'S buffer solution MOPS (3-(N-morpholine) propanesulfonic acid) (MOPS is the main component of buffer solution), and described buffer solution pH value is 7.0;

[0058] Reagent 2 is as follows:

[0059]

[0060] The rest is GOOD'S buffer MOPS, and the buffer pH value is 7.0.

Embodiment 3

[0062] The low-density lipoprotein cholesterol detection kit raw material and proportioning of the present embodiment are as follows:

[0063] The kit consists of reagent 1 and reagent 2;

[0064] Reagent 1 is as follows:

[0065]

[0066] All the other are GOOD'S buffer solution MOPS, and the buffer solution pH value is 7.0;

[0067] Reagent 2 is as follows:

[0068]

[0069] The rest is GOOD'S buffer, the buffer pH value is 7.0.

[0070] The compound stabilizer adopted in the above-mentioned embodiment 1-3 comprises Tween 80 10~13g / L, glutamic acid 20~30g / L, glutathione 1~3g / L, trehalose 50~60g / L, ten Dialkyl dimethyl betaine 2-6g / L, lecithin 3-6g / L, buffer solution 100-120mmol / L; it is a complex stabilizer of various antibodies and its application method with application number 201610166107.2 Composite stabilizer.

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Abstract

The invention discloses a detection kit for LDL (low-density lipoprotein) cholesterol and a use method of the detection kit, relates to the field of biochemical detection and aims to provide a detection kit, having high stability, accuracy and precision as well as low toxicity, for LDL cholesterol and a use method of the detection kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 is prepared from a surfactant, polyethylene glycol, SDS (sodium dodecyl sulfate), Emulgen A9 series, CHOD (cholesterol oxidase), CHER (cholesterol esterase), ascorbic acid oxidase, CAT (catalase), 4-AAP (4-aminoantipyrine) and the like; the reagent 2 is prepared from octylphenyl polyethylene glycol, cholate, a compound stabilizer, bovine serum albumin, POD (peroxidase), TOPS (sodium 3-(N-ethyl-3-methylanilino)propanesulfonate) and the like. The proclin series added to the kit is a novel biological preservative, has good compatibility with various enzymes, better stability and low toxicity, and the stability of the reagents is maintained.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a kit for measuring materials by means of measuring their chemical and physical properties. Background technique [0002] In addition to free fatty acids bound to albumin, lipids present in the blood are incorporated into the structure of lipoproteins, and they form as chylomicrons (CHM), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), It exists in the form of high-density lipoprotein (HDL), among others. Cholesterol is particularly distributed in VLDL, LDL and HDL. LDL is known to function to transport cholesterol, and high levels of LDL promote arteriosclerosis. It is known that measurement of low-density lipoprotein cholesterol (LDL-C) in blood becomes an indicator of occurrence and progression of arteriosclerosis. [0003] Burstein M et al. (Rapid Method for the Isolation of Lipoproteins from Human Serum by Precipitation with Polyanions, J. Lipid Re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 孙文勇常俊骏郑佳
Owner WHITMAN BIOTECH NANJING
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