Method for distinguishing flavor substance in edible mushroom through combination of headspace gas chromatography-mass spectrometer and electronic nose
A headspace gas chromatography and flavor substance technology, applied in instruments, scientific instruments, measuring devices, etc., can solve problems such as comprehensive reflection of the overall flavor characteristics of volatile substances in inedible fungi, and achieve the effect of accurate identification and determination
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[0036] A method for identifying flavor substances in edible fungi using headspace gas chromatography-mass spectrometry combined with an electronic nose, specifically comprising the following steps:
[0037] (1) Sample preparation:
[0038] Take commercial dried shiitake mushrooms and Grifola frondosa to be identified, dry them at 80°C for 4 hours, grind them mechanically, pass through a 40-mesh sieve, and extract them with steam distillation for 2.5 hours under the condition of solid-liquid ratio of 1:10. Obtain the essential oil samples of shiitake mushroom and frondosa frondosa respectively;
[0039] (2) Carry out headspace-gas chromatography-mass spectrometry analysis and determination of the essential oil samples of shiitake mushrooms and frondosa frondosa obtained in step (1), the specific process is as follows:
[0040] ①. Take 8ml of essential oil samples of Lentinus edodes and Grifola frondosa respectively, put them into 15ml sample containers, and put them in a 50°C ...
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