69 results about "Radial chromatography" patented technology

A method for separating and analyzing volatile and semi-volatile flavor components in tobacco by using liquid chromatography-gas chromatography / mass spectrometry technology comprises the following steps: 1. taking a given weight of tobacco products and extracting the volatile and semi-volatile flavor components from the tobacco by simultaneously adopting distillation and extraction; 2. concentrating the extracted samples with a concentrator; 3. carrying out group component separation on the concentrated extracted samples by normal-phase liquid chromatography and collecting distillates by different time sections; and 4. concentrating the collected distillates by the concentrator and respectively entering a gas chromatography-mass spectrometer (GC-MS) for detailed analysis. Compared with theprior art, the method of the invention can effectively extract the volatile and semi-volatile flavor components from the tobacco, carry out group component separation on the extract liquor by normal-phase liquid chromatography, then carry out accurate GC-MS qualitative and quantitative analysis on each group component according to different boiling points, not only has good selectivity and high separation efficiency but also ensures more accurate qualitative and quantitative analysis of each component.

The invention provides an interface device for on-line coupling of LC-GC / MS and a method for analyzing fragrant components in tobaccos by utilizing the device. According to the method, reverse blowing gas can be provided through switch of a gas switch valve, during sample injection, the reverse blowing gas is discharged form a GC flow-splitting port against the GC carrier gas direction through a tee coupling, therefore solvents can be transferred out completely, solutes are reserved in a TenaxTA absorbent in a PTV liner tube, thermal desorption is performed on a PTV injection port after the solvents are transferred completely, the GC carrier gas shifts cut fractions to be tested into a GC column for separation, effects on an MS vacuum system due to the fact the solvents enter the GC column are effectively avoided, and large volume injection can be achieved.

The invention relates to a needle type extraction device suitable for gas chromatography and a using method. The extraction device consists of a heat resistant stainless steel needle head and a section of adsorbents filled inside a needle cylinder, wherein the adsorbents serving as extraction media are used for absorbing a trace amount of volatile or semi-volatile organic compounds; and adsorbent microspheres are fixed inside the needle cylinder by using heat resistant glass cotton. A needle bed of the device can be connected with a simple gas pump or an exchangeable needle head type hermetically sealed injector to extract and concentrate a target material; after the extraction, the device is combined with the gas chromatography to realize thermal desorption under the condition of inert gas serving as a carrier gas and a high temperature of a vaporizing chamber so as to perform the separation and detection in the gas chromatography. The device can also store a sample by sealing the two ends of the device with a Teflon seal head for subsequent analysis when a field test is unavailable. The extraction device can be applied to the analysis of the fast extraction of a trace amount of gas samples in the chemical field.

The invention discloses a method for simultaneously measuring 28 kinds of pesticide residues with gas chromatography-triple quadrupole tandem mass spectrometry. The method includes the steps of A, sample pretreatment, namely weighing fresh tea leaf powder and pouring the same into a centrifuge tube, adding 1% of an acetonitrile acetic acid solution, adding NaCl after homogenated extraction, and performing full shock blending and centrifugal stratifying prior to taking clear liquid on the upper layer for standby use; B, purification and interior label treatment, namely pouring the clear liquid on the upper layer into the centrifuge tube, purifying the clear liquid with QuEChERS and adding an interior label solution, and taking supernate for standby use after whirlpools shock and centrifugation; C, mass spectrometric measurement, namely subjecting the supernate in the step B to qualitative and quantitative analysis with the gas chromatography-triple quadrupole tandem mass spectrometry. With the method, the problem about measurement blank of the pesticide residues of tea leaves is solved, and the method has the advantages of high flexibility, good selection and high repeatability and accuracy, the 28 kinds of pesticide residues in the green leaves can be measured simultaneously, and the method is specially suitable for measurement analysis for large amount of samples.

The invention relates to the fields of pesticide adjuvant residue detection, drug detection, environmental safety and the like, in particular to a method for detecting residual organic solvents in soil and water by adopting a gas chromatography-mass spectrometry method. The method comprises the following steps that 1, the soil and water to be detected are respectively subjected to solvent extraction and anhydrous Na2SO4 pretreatment and then are centrifuged, supernatant is obtained and filtered to obtain an extraction solution; 2, the gas chromatography-mass spectrometry is adopted to detect the extraction solution in the step 1, wherein a used gas chromatography column is a DB-WAX capillary chromatographic column, and MgSO4 is added in the extraction solution before the chromatographic column is used. Organic solvents include toluene, xylene, DMF and DMSO. The xylene includes dimethylbenzene, paraxylene and dimethylbenzene. The method is fast, convenient and accurate and sensitively detects organic solvent residue quantity in environmental samples such as soil and water.

The invention discloses a method for separating an anti-tumor polysaccharide component from a black fungus by applying radial chromatography. The method comprises the following steps of: 1) extracting the black fungus with hot water; 2) adding Savag agent after concentrating the extracting liquid of the black fungus polysaccharide, shaking and centrifuging; 3) adding alcohol with the volume concentration being more than or equal to 95% into a polysaccharide aqueous solution free from protein, performing alcohol precipitation, and cleaning the obtained precipitation; 4) carrying out freeze drying on coarse black fungus polysaccharide with anti-tumor activity; 5) dissolving the freeze-dried coarse black fungus polysaccharide in distilled water, thereby obtaining a sample solution; pumping the sample solution into a radial chromatograph, selecting DEAE-52 cellulose as padding; respectively eluting by using the distilled water and NaCl solution with the concentration of 0.2M; and 6) collecting the eluent corresponding to the NaCl solution with the concentration of 0.2M, and performing dialysis, concentration and drying in turn, thereby obtaining the black fungus polysaccharide with anti-tumor activity.

The present invention provides a gas chromatography—inverse gas chromatography combined analysis device, which includes a gas chromatography column and an inverse gas chromatography column, an input end of the gas chromatography column is connected to a sample feeder, an output end of the gas chromatography column is connected to an input end of the inverse gas chromatography column, the output end of the gas chromatography column is further connected to a first detector, the input end of the inverse gas chromatography column is further connected to a carrier gas tube, an output end of the inverse gas chromatography column is connected to a second detector, and the first detector and the second detector are both connected to a signal collector. The present invention not only can investigate adsorption performance of a tested solid adsorption material with respect to a single probe, but also can investigate adsorption performance of different solid adsorption materials with respect to different constituents in a combined probe at the same time, thereby improving the development efficiency of the inverse gas chromatography technologies.

The invention relates to a method for detecting fosfomycin sodium in pharmaceutical wastewater by adopting an ion chromatography. The method comprises the following steps that (1) chromatographic condition: chromatographic columns include a low-volume hydroxyl system cathodic protection column and a high-volume anion exchange separation column, a suppressor is an electrolytic film suppressor, an electrical conductivity detector is used, and leacheate is a sylvite solution; (2) the preparation of a standard solution: a standard product of the fosfomycin sodium is mixed with ultrapure water to obtain into the standard solution; (3) the pretreatment of a sample: a sample to be detected is filtered by using a 0.45mum filtering film and then processed by a C18 colonnette so that organic matters in the water sample are removed; (4) a detecting method: the standard solution is added in an ion chromatograph, then a spectrogram is recorded, the position of a standard peak is determined, the area of a fosfomycin sodium peak is calculated, and a standard curve is drawn according to the calculated area of the peak; a testing sample is added in the ion chromatograph, then a spectrogram is recorded, and the concentration of the sample is calculated according to the area of the fosfomycin sodium peak. The method provided by the invention has the advantages that the detection repeatability of the fosfomycin sodium in the pharmaceutical wastewater is good, the detection limit is low, the sensitivity is high, the operation is simple, the detecting condition is gentle, the detection equipment and instrument are widely applied, the source of a leacheate sylvite solution is wide, and the configuration is simple, and the method is widely used for detecting fosfomycin sodium in the industrial wastewater.

Provided are two-dimensional chromatography systems and methods for separating and / or analyzing complex mixtures of organic compounds. In particularly, a two-dimensional reversed-phase liquid chromatography (RPLC)-supercritical fluid chromatography (SFC) system is described including a trapping column at the interface which collects the analytes eluted from the first dimension chromatography while letting the RPLC mobile phase pass through. The peaks of interest from the RPLC dimension column are effectively focused as sharp concentration pulses on the trapping column, which is subsequently injected onto the second dimension SFC column. The system can be used for simultaneous achiral and chiral analysis of pharmaceutical compounds. The first dimension RPLC separation provides the achiral purity result, and the second dimension SFC separation provides the chiral purity result (enantiomeric excess).