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A continuous extraction method of effective ingredients from fruitbodies of Cordyceps militaris

A technology for Cordyceps sinensis seeds and active ingredients, which is applied in the field of extraction of biologically active substances, can solve the problems of loss of active ingredients, high product cost, and difficulty in development, and achieves the effect of reducing industrialization costs.

Inactive Publication Date: 2007-08-01
SHANGHAI ELITE AGRI SCI TECH GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction of a single active ingredient causes a large number of other active ingredients in the fruiting body of Cordyceps militaris to be lost in vain, making it difficult to develop multiple physiologically active ingredients in the fruiting body of Cordyceps militaris or biologically active substances with multiple clinical uses, resulting in a single product. Product cost is too high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0016] 1. CO 2 supercritical fluid extraction

[0017] Take 700 grams of Cordyceps militaris fruiting bodies, grind them into powder, put them into the extraction kettle and keep it at 60°C, the pressure is 400bar, and feed CO 2 , flow rate is 30g / min, add CO 2 The 95% ethanol of 10% content is made cosolvent, equilibrates 20min, and extraction time is 2h, obtains light yellow oily extract and residue, uses reverse phase high performance chromatography (chromatographic column: Kromasil ODS C 18 4.6×250mm, 5um; mobile phase: methanol: water = 15:85; flow rate: 0.8ml / min; detection wavelength: 260nm; detection temperature: 30°C; Changes of cordycepin content.

[0018] 2. Alcohol precipitation

[0019] Put the residue obtained in the above steps into an extraction tank for extraction, add 1200ml of distilled water, incubate at 70°C for 2 hours, take it out and centrifuge, put the residue back into distilled water, repeat the extraction three times, combine the three extracts...

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PUM

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Abstract

The invention disclosed a way to successively extract active ingredients from carpohole of north aweto. The invention adopts super critical CO2 to extract sterin substances, precipitating with alcohol to get nucleoside substances with cordycepin and Cordyceps polyose, separating with normal phase low pressure gel silica chromatography to get crude cordycipin, preparative reversed-phase high-performance liquid chromatography to get cordycepin crystal with high purity. The invention can not only successively extract sterin substances and Cordyceps polyose (CM-1) from the carpohole of north aweto, it can also get the cordycepin metamer; it is not applicable to small experiments but also applicable to industrial production. Since the producers can extract many substances by one treatment of carpohole, the invention can not only decrease the industrial cost but also make the biggest economic returns.

Description

technical field [0001] The invention relates to a method for extracting biologically active substances, in particular to a method for continuously extracting active ingredients from fruiting bodies of Cordyceps militaris. Background technique [0002] The active ingredients in the fruiting bodies of Cordyceps militaris include sterol compounds, cordyceps polysaccharides, cordycepin, etc. At present, domestic enterprises or scientific research institutes mostly use single-component extraction methods for the extraction of active ingredients in the fruiting bodies of Cordyceps militaris. This method is Maximize the yield of target components, and other components as impurities, do not pay attention to the loss. In addition, when extracting a single active ingredient, the raw materials used are the original fruiting body of Cordyceps militaris, and the process is relatively simple and easy to master. The extraction of a single active ingredient causes a large number of other a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/068
CPCY02P20/54
Inventor 陈伟高新华唐乐珊沈明吴畏
Owner SHANGHAI ELITE AGRI SCI TECH GROUP
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