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Preparation of high-purity Momordica grosvenori mogroside V

A mogroside, high-purity technology, applied in the directions of steroids, organic chemistry, etc., can solve the problems of affecting the purity of the finished product, complicated operation, high cost, shorten the production cycle, improve the quality, reduce the difficulty of production operation and production cost. Effect

Inactive Publication Date: 2009-04-15
桂林市振达生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above preparation methods can obtain mogroside V with a purity greater than 90%, they all need to pass column chromatography twice through a normal-phase silica gel column and a reverse-phase silica gel column, which has many procedures, complicated operation, and correspondingly high cost , and the eluent mixed with water and other organic solvents will elute impurities together, thus affecting the purity of the finished product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1) Dissolve 2 kg of mogroside (the content of mogroside V is 60%) in 10 liters of methanol, and filter to obtain the filtrate;

[0014] 2) Add an equal volume of acetone to the resulting filtrate to produce a precipitate, filter to obtain a precipitate;

[0015] 3) Adsorb the precipitate with a normal phase silica gel column; the weight ratio of the precipitate to silica gel is 1:30;

[0016] 4) The adsorbed normal phase column is eluted with a mixed solution of ethyl acetate and ethanol as the eluent, and the flow rate of the eluent is 3ml / min, and the eluent is collected; wherein, the volume ratio of ethyl acetate to ethanol is 2:1;

[0017] 5) Recover the eluent in the eluent to obtain a coarse powder, add methanol to the coarse powder, and heat to 50°C to dissolve it. The ratio of the volume of methanol to the weight of the coarse powder is 10:1, and then add 2 Acetone twice the volume of methanol, then cooled naturally, left until crystallized, and then recrystal...

Embodiment 2

[0019] 1) Take 700 grams of mogroside (the content of mogroside V is 50%) and dissolve it in 5L of methanol, filter to obtain the filtrate;

[0020] 2) Add an equal volume of acetone to the resulting filtrate to produce a precipitate, filter to obtain a precipitate;

[0021] 3) Adsorb the precipitate with a normal phase silica gel column; the weight ratio of the precipitate to silica gel is 1:70;

[0022] 4) Adsorbed normal phase column, use the mixed solution of isopropyl acetate and ethanol as eluent to elute, and the flow rate of eluent is 5ml / min, collect eluent; Wherein, the mixture of isopropyl acetate and ethanol The volume ratio is 3:1;

[0023] 5) Recover the eluent in the eluent to obtain a coarse powder, add methanol to the coarse powder, heat to 50°C to dissolve it, the ratio of the volume of methanol to the weight of the coarse powder is 10:1, and then add 7 Acetone twice the volume of methanol, then cooled naturally, left until crystallized, and then recrystall...

Embodiment 3

[0025] 1) Dissolve 500 grams of mogroside (the content of mogroside V is 30%) in 5 L of methanol, filter to obtain the filtrate;

[0026] 2) Add an equal volume of acetone to the resulting filtrate to produce a precipitate, filter to obtain a precipitate;

[0027] 3) Take the precipitate and adsorb it with a normal phase silica gel column; the weight ratio of the precipitate to silica gel is 1:50;

[0028] 4) Adsorbed normal phase column, use the mixed solution of n-propyl acetate and ethanol as eluent to elute, and the flow rate of eluent is 2ml / min, collect eluent; Wherein, the mixed solution of n-propyl acetate and ethanol The volume ratio is 5:1;

[0029] 5) Recover the eluent in the eluent to obtain a coarse powder, add methanol to the coarse powder, and heat to 50°C to dissolve it. The ratio of the volume of methanol to the weight of the coarse powder is 10:1, and then add 4 Acetone twice the volume of methanol, then cooled naturally, left until crystallized, and then ...

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PUM

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Abstract

The invention discloses a preparation method of grosvenor momordica glycoside V with high purity, which has steps as follows: 1) the grosvenor momordica glycoside is dissolved in methanol and then filtered to obtain a filtrate; 2) acetone is added in the obtained filtrate for sedimentation and a sediment can be obtained by filtering; 3) the sediment is adsorbed by a normal phase silica gel column; 4) the adsorbed normal phase silica gel column is eluted by using a mixing solution of low carbon chain ester and ethanol as an elution agent, and elution liquid is collected; and 5) the grosvenor momordica glycoside V with high purity can be obtained by recycling and crystallizing the elution agent in the elution liquid. Compared with the prior art of twice column adsorbing methods of the normal phase silica gel column and expensive opposite phase silica gel column, the preparation method only needs separation and elution of the normal phase silica gel column once to obtain the grosvenor momordica glycoside V with good quality and purity of more than 98 percent. Compared with the prior art, the preparation method of the grosvenor momordica glycoside V with high purity is simpler in technique, shortens the production cycle and reduces the production operation difficulties and production cost.

Description

(1) Technical field: [0001] The invention relates to a method for extracting active ingredients from natural plants, in particular to a method for preparing high-purity mogroside V. (two) background technology: [0002] Luo Han Guo is the fruit of perennial herbaceous vines belonging to Cucurbitaceae. It is a unique plant in southern my country. It is mainly produced in northern Guangxi. The quality of Luo Han Guo in Guilin, Guangxi is the best. Luo Han Guo has been eaten as traditional Chinese medicine and dried fruit for a long time. It tastes extremely sweet, cool in nature, non-toxic, and enters the spleen and lungs. Quenches thirst, promotes gastrointestinal function, suppresses asthma and lowers blood pressure. Since it was first reported in 1975 that the fruit of Luo Han Guo contained diterpene sweetosides, mogroside IV (mogroside IV), mogroside V, mogroside III, mogroside IIE, mogroside IIIE, Mogroside VI, mogroside A, mogroside (nemogroside), siamenside I (siamens...

Claims

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Application Information

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IPC IPC(8): C07J17/00
Inventor 孙莉莉韦海洪唐江明李振志
Owner 桂林市振达生物制药有限公司
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