The invention discloses a 400 MPa level fine grain threaded reinforcing bar and a manufacturing method thereof, and belongs to the field of threaded reinforcing bar production. The reinforcing bar comprises, by mass percentage, 0.20-0.25% of C, 0.3-0.8% of Si, 1.2-1.6% of Mn, 0.01-0.04% of P, 0.01-0.04% of S, 0.004-0.02% of Ti, 0.001-0.08% of Cr, 0.0001-0.008% of V, 0.0001-0.008% of Nb, 0.0001-0.008% of Al, 0.003-0.01% of O, 0.003-0.01% of N and the balanced of Fe and unavoidable impurities; manganese silicate inclusion, titanium oxide manganese sulfide complex inclusion, manganese sulfide complex inclusion and other unavoidable inclusions are included. The method comprises the steps of molten steel smelting, inclusion controlling, continuous casting, continuous casting blank heating and rolling cooling. The method has the advantages that optimizing designing is conducted on components and the inclusions, smelting and rolling are combined, a reinforcing mechanism of using the inclusions to induce fine crystalline to improve the strength of the reinforcing bar, the addition of precious alloy elements is reduced, and therefore the low-cost high-quality production of the threaded reinforcing bars is achieved.