Preparation of high-purity Momordica grosvenori mogroside V

A mogroside, high-purity technology, applied in the directions of steroids, organic chemistry, etc., can solve the problems of affecting the purity of the finished product, complicated operation, high cost, shorten the production cycle, improve the quality, reduce the difficulty of production operation and production cost. Effect

Inactive Publication Date: 2011-05-25
桂林市振达生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above preparation methods can obtain mogroside V with a purity greater than 90%, they all need to pass column chromatography twice through a normal-phase silica gel column and a reverse-phase silica gel column, which has many procedures, complicated operation, and correspondingly high cost , and the eluent mixed with water and other organic solvents will elute impurities together, thus affecting the purity of the finished product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1) Take 2 kg of mogroside (mogroside V content is 60%), dissolve it in 10 liters of methanol, filter, and obtain the filtrate;

[0014] 2) Add an equal volume of acetone to the obtained filtrate to cause precipitation, and filter to obtain a precipitate;

[0015] 3) Adsorb the precipitate with a normal phase silica gel column; the weight ratio of the precipitate to the silica gel is 1:30;

[0016] 4) The adsorbed normal phase column is eluted with a mixed solution of ethyl acetate and ethanol as the eluent, the eluent flow rate is 3ml / min, and the eluent is collected; among them, the volume ratio of ethyl acetate and ethanol 2:1;

[0017] 5) Recover the eluent in the eluent to obtain coarse powder. Add methanol to the coarse powder and heat to 50°C to dissolve it. The ratio of the volume of methanol to the weight of the coarse powder is 10:1. After dissolving, add 2 The acetone that is twice the volume of methanol is then naturally cooled and allowed to stand until it crystall...

Embodiment 2

[0019] 1) Dissolve 700 grams of mogroside (Mogroside V content is 50%) in 5L methanol, filter, and obtain the filtrate;

[0020] 2) Add an equal volume of acetone to the obtained filtrate to cause precipitation, and filter to obtain a precipitate;

[0021] 3) Adsorb the precipitate with a normal phase silica gel column; the weight ratio of the precipitate to the silica gel is 1:70;

[0022] 4) The adsorbed normal phase column is eluted with a mixed solution of isopropyl acetate and ethanol as the eluent, the eluent flow rate is 5ml / min, and the eluent is collected; among them, the mixture of isopropyl acetate and ethanol The volume ratio is 3:1;

[0023] 5) Recover the eluent in the eluent to obtain coarse powder. Add methanol to the coarse powder and heat to 50°C to dissolve it. The ratio of the volume of methanol to the weight of the coarse powder is 10:1. After dissolving, add 7 The acetone that is twice the volume of methanol is then naturally cooled and allowed to stand until it...

Embodiment 3

[0025] 1) Take 500 grams of mogroside (mogroside V content is 30%), dissolve it in 5L methanol, filter, and obtain the filtrate;

[0026] 2) Add an equal volume of acetone to the obtained filtrate to cause precipitation, and filter to obtain a precipitate;

[0027] 3) Adsorb the precipitate with a normal phase silica gel column; the weight ratio of the precipitate to the silica gel is 1:50;

[0028] 4) The adsorbed normal phase column is eluted with a mixed solution of n-propyl acetate and ethanol as the eluent, the eluent flow rate is 2ml / min, and the eluent is collected; among them, the mixture of n-propyl acetate and ethanol The volume ratio is 5:1

[0029] 5) Recover the eluent in the eluent to obtain coarse powder. Add methanol to the coarse powder and heat to 50°C to dissolve it. The ratio of the volume of methanol to the weight of the coarse powder is 10:1. After dissolving, add 4 The acetone that is twice the volume of methanol is then naturally cooled and allowed to stand un...

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Abstract

The invention discloses a preparation method of grosvenor momordica glycoside V with high purity, which has steps as follows: 1) the grosvenor momordica glycoside is dissolved in methanol and then filtered to obtain a filtrate; 2) acetone is added in the obtained filtrate for sedimentation and a sediment can be obtained by filtering; 3) the sediment is adsorbed by a normal phase silica gel column; 4) the adsorbed normal phase silica gel column is eluted by using a mixing solution of low carbon chain ester and ethanol as an elution agent, and elution liquid is collected; and 5) the grosvenor momordica glycoside V with high purity can be obtained by recycling and crystallizing the elution agent in the elution liquid. Compared with the prior art of twice column adsorbing methods of the normal phase silica gel column and expensive opposite phase silica gel column, the preparation method only needs separation and elution of the normal phase silica gel column once to obtain the grosvenor momordica glycoside V with good quality and purity of more than 98 percent. Compared with the prior art, the preparation method of the grosvenor momordica glycoside V with high purity is simpler in technique, shortens the production cycle and reduces the production operation difficulties and production cost.

Description

(1) Technical field [0001] The invention relates to a method for extracting effective ingredients from natural plants, in particular to a method for preparing high-purity mogroside V. (2) Background technology: [0002] Luo Han Guo is the fruit of perennial herbaceous vines in the Cucurbitaceae family. It is an endemic plant in southern my country and is mainly produced in northern Guangxi. The quality of Luo Han Guo is the best in Guilin, Guangxi. Luo Han Guo has been eaten as a traditional Chinese medicine and beverage dried fruit for a long time. Its taste is extremely sweet, cool in nature, non-toxic, and enters the spleen and lungs. It quenches thirst, promotes gastrointestinal function, suppresses asthma and lowers blood pressure. Since it was first reported in 1985 that mogroside fruits contain diterpene sweet glycosides, mogroside IV (mogroside IV), mogroside V, mogroside III, mogroside IIE, and mogroside have been isolated and identified from mogroside fruits. IIIE, mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J17/00
Inventor 孙莉莉韦海洪唐江明李振志
Owner 桂林市振达生物制药有限公司
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