34results about How to "Conducive to industrial production" patented technology

The invention provides a method for preparing 1, 3-methyl-5-(2, 6, 6-trimethyl-1- cyclohexene-1-yl)-2, 4-pentadiene methyl phosphonate. In the method, a benzophenone compound preventing transposition is added in the process of generating the 1, 3-methyl-5-(2,6,6-trimethyl-1-cyclohexene-1-yl)-2,4-pentadiene methyl phosphonate by the reaction of methylene diphosphate four alkyl and C14 aldehyde in a mixed solvent of methylbenzene and tetrahydrofuran, thereby inhibiting the generation of a target product isomer in order that the content of the target product in a final product reaches up to 97 percent.

The invention belongs to the technical field of textile fiber modification and relates to a method for preparing flame-retardant melt-proof fibers. The method comprises the following steps: slicing and drying matrix resin, adding anti-dripping agent powder and a flame retardant into the slices, fully mixing, and further blending, extruding, cooling and granulating through a twin-screw extruder so as to obtain a flame-retardant melt-proof master batch; and slicing and drying another matrix resin, conveying the flame-retardant melt-proof master batch to be mixed with the previous matrix resin slices by using an injection machine, fully melting and mixing through a spinning screw, and spinning, thereby obtaining the flame-retardant melt-proof fibers. The anti-dripping agent and the flame retardant are directly added into the matrix resin slices, and a molten drop phenomenon is avoided during combustion of the prepared flame-retardant melt-proof fibers. Moreover, the conventional spinning process of the fibers is not influenced by a blending melt spinning method, the process flow is simple, and industrial production is facilitated.

The invention discloses a cyclodextrin glucosyltransferase mutant weakened via product inhibition, belonging to the fields of gene engineering and enzyme engineering. By using a site-specific mutagenesis method, the cyclodextrin production capacity of a CGTase is improved, and a mutation scheme for strengthening the cyclodextrin production capacity of a beta-CGTase from Bacillus circulans STB01 is provided to obtain a mutant A599N or A599N / Y633A. Compared with the wild CGTase, the cyclodextrin production capacity of the mutant is remarkably strengthened so that the mutant is more suitable for the industrial production of cyclodextrin.

The invention discloses a cyclodextrin glucosyltransferase and a preparation method thereof, and belongs to the field of genetic engineering and enzyme engineering. Through replacement of 156-site Alanine (Ala) of CGTase of P. Macerans strain JFB05-01 (CCTCC NO: M208063) respectively with lysine (Lys), glutamine (Gln) and valine (Val), the genistein glycosylation efficiency is increased by 23%, 44% and 32%, respectively. The mutant enzymes are more conductive to catalysis production of glycosylated genistein compared with wild-type CGTase.

The invention discloses bacillus licheniformis which is classified and named as Bacilluslicheniformis 7172 and is preserved in the common microorganism center of the microorganism strain preservation management committee in China with the preservation number of CGMCC NO.7172 in Beijing, China at January 18th in 2013. According to the bacillus licheniformis and application thereof, vanillin is produced by biologically converting ferulic acid by using the bacillus licheniformis under a high temperature condition; the growth of infectious microbes can be effectively controlled; and the industrial production is facilitated. Furthermore, by the method, the environment pollution is low, the production period is short, and a product is environment-friendly, safe and reliable; the long-time shortcoming caused by the production of the vanillin by a chemical synthesis method is overcome; and the bacillus licheniformis has a wide application prospect.

The invention discloses herba taraxaci composite enzyme as well as a preparation method and an application thereof. The preparation method comprises the steps as follows: (1) raw materials including herba taraxaci and herba houttuyniae are crushed, 40-50 parts by weight of herba taraxaci and 50-60 parts by weight of herba houttuyniae are taken respectively and mixed with water, and a suspension isobtained; (2) the suspension is subjected to enzymelysis with pectinase, cellulase, xylanase and trypsin, and an enzymatic hydrolysate is obtained; (3) nutrient substances are added to the enzymatichydrolysate, sterilization is performed, and a culture medium is obtained; (4) the culture medium is inoculated with saccharomyces for anaerobic fermentation culture for 18-30 h and then inoculated with lactic acid bacteria for still standing fermentation culture for 30-120 h, and a cultured product is obtained; (5) the cultured product is subjected to solid-liquid separation, and the composite enzyme is obtained. The fermentation time can be shortened with the method and the obtained composite enzyme has the more significant antibacterial effect.

The invention relates to a mutation method for enhancing beta-cyclodextrin production capacity of beta-cyclodextrin glycosyltransferase (short for beta-CGT enzyme), and belongs to the field of gene engineering and enzyme engineering. According to the present invention, a site-specific mutagenesis method is adopted to increase beta-cyclodextrin production capacity of the CGT enzyme, the mutation scheme for enhancing beta-cyclodextrin production capacity of the beta-CGT enzyme derived from Bacillus circulans STB01 is provided, mutation of alanine on the site 31 in the CGT enzyme into arginine (Arg), proline (Pro) or threonine (Thr) is performed to obtain mutants A31R, A31P and A31T, beta-cyclodextrin production capacity of the mutant is significantly enhanced compared with beta-cyclodextrin production capacity of the wild CGT enzyme, and the mutation method is more suitable for beta-cyclodextrin industrial production.

The invention relates to a fondaparinux sodium pentasaccharide intermediate in the technical field of chemical preparation and a preparation method thereof, particularly to a preparation method of the fondaparinux sodium pentasaccharide intermediate, and comprises a trisaccharide intermediate of fondaparinux sodium and preparation method thereof. The invention designs a novel synthetic route to prepare the fondaparinux sodium pentasaccharide intermediate. The route has mild reactions, good reaction selectivity, strong controllability and low operation difficulty, and is beneficial to achievement of industrial production. The route has a low cost so that the method can meet requirements of large-scale industrial production.

The invention relates to an intermediate of Proellex(R)-V and preparation method and purpose thereof. The invention provides a 4-step method for preparing 3,3,20,20-di(ethylenedioxy)-17alpha,21-dihydroxy-19-norpregna-5(10),9(11)-diene, which has the advantages of mild reaction condition, easily controlled operation process, easily available raw materials and reagents, low cost and high yield, and the method is suitable for industrial production, and the obtained 3,3,20,20-di(ethylenedioxy)-17alpha,21-dihydroxy-19-norpregna-5(10),9(11)-diene can be used for further preparing Proellex(R)-V.

A process for preparing a Ziegler-Natta catalyst used for the sludge, bulk and gas-phase polymerization of propene features that in its preparing procedure, the small solid particles as special treater are added for controlling the growth of catalyst particles, so controlling the form and size of propene polymer.

The invention provides a synthetic method for a small-crystal-size high-silicon Y-type molecular sieve. The method comprises the following steps: stirring water, inorganic base, an aluminum source and part of a silicon source for 2-4 days; then adjusting the basicity of the system with acid; and adding a silicon source, and finally synthesizing the Y-type molecular sieve by hydrothermal crystallization. The small-crystal high-silicon Y-type molecular sieve synthesized by the method provided by the invention has the characteristics of small crystal size and high silicon-aluminum ratio, is suitable for serving as an adsorbent for separation of a gas and liquid mixture and can also serve as a catalyst carrier or an acid catalyst component.

The invention relates to the technical field of pesticide production, in particular to a purifying method of rimsulfuron original medicine. The purifying method comprises the following steps: (1) adding a rimsulfuron original medicine crude product into a solvent, and heating under a stirring condition; (2) adding or not adding a solvent, slowly cooling, filtering after completely separating out aproduct, and taking filter cake; (3) adding the filter cake in the solvent, and stirring and pulping; (4) filtering and drying, thus obtaining a purified rimsulfuron original medicine product. According to the purifying method disclosed by the invention, through selection of a new purifying method, proportions of main purities can be reduced, the purity of original medicine can be increased, thepurity of the rimsulfuron original medicine purified by utilizing the technology of the invention is not lower than 98.5 percent, the content of purities is reduced to a large extent, and the problemof low purity generally existing in the industry is solved; meanwhile, the technical scheme of the invention is very easy to operate, the purification cost is relatively low, and the purifying methodis beneficial for being adopted in industrial production.

The present invention relates to the field of chemical industry, and in particular to a method for splitting D,L-menthol. The method for splitting D,L-menthol comprises the steps of: (1) dissolving D-menthyl acrylate or L-menthyl acrylate with optical purity of 40%-90% in an organic solvent, cooling and stirring to precipitate solid; and (2) subjecting the precipitated solid to alkali hydrolysis to obtain the optically pure D- menthol or L-menthol. The step (1) can also be replaced with a step of forming a supersaturated solution of D-menthyl acrylate or L-menthyl acrylate with optical purity of 40%-90% in an organic solvent, cooling, stirring, adding seed crystals for inducing crystallization and precipitating solid. The D-menthol or L-menthol obtained by the method provided by the invention has optical purity higher than 98%, and the problem of insufficient optical purity caused by chiral induction conventional crystallization or chiral induction crystallization is well solved.

The invention relates to a Cu-doped MOF-5 catalyst and a preparation method and application thereof. The preparation method includes the following steps: mixing zinc salt, copper salt, a solvent, terephthalic acid and a catalyst and reacting to obtain MOF-5(Cu); and then calcining the MOF-5(Cu) to obtain the Cu-doped MOF-5 catalyst. By doping the MOF-5 with copper, catalytic active sites are combined with the MOF-5 firmly, and the Cu-doped MOF-5 catalyst with excellent stability can be obtained. The preparation method has a simple process and is beneficial to industrial production.

The invention discloses a steel-concrete combined short-leg shear wall. Steel tube columns are arranged on the end and the corner of the short-leg shear wall. Steel plates are symmetrically arranged between the adjacent steel tube columns on the short-leg shear wall, the two ends of the steel plates are fixedly connected with the steel tube columns, and interiors of the steel tube columns and a cavity formed between the steel plates and the steel tube columns are filled with concrete. The shear wall is simple in structural manner, industrial production is facilitated, the manner of combining steel tubes, the steel plates and concrete is adopted, the structural rigidity can be improved, the anti-seismic property is improved, the use amount of the steel tubes is small, and economy is good.

The invention provides a method for preparing 2-[(6-chloro-3-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl]-4-fluorobenzonitrile, and belongs to the technical field of pharmacy. The method comprises: performing bromination reaction on 4-fluoro-2-methylbenzonitrile and a bromination reagent to obtain a first product, then reacting the first product with 6-chloro-3-methyluracil in an alkali and a solvent for a period, then adding a catalyst, and performing reaction and post-processing, so as to obtain the target product. The catalyst is selected from one or more of diethyl phosphite, dimethyl phosphite and diphenyl phosphite. The provided method does not need to separate and purify 2-bromomethyl-4-fluorobenzonitrile, and is capable of obtaining the high-purity product at a high yield by adding the catalyst, and is low in cost and suitable for industrial production.

The invention provides nutrient supplement infant rice flour rich in easily absorbable zinc trace elements. A preparation method comprises the following steps: taking cassava starch as a main raw material, carrying out homogeneous debranching treatment on the starch by adopting a biological enzyme technology, recombining a supramolecular structure of starch molecules to obtain amylose with a spiral cavity, and then adding zinc ions to enable the amylose to inclusion complex zinc elements to construct a starch metal complex; and taking 20-35 parts of the starch metal complex and auxiliary materials including 3-5 parts of whole milk powder, 1-2 parts of glucose, 0.2-0.6 part of composite vitamins, 0.2-0.6 part of composite minerals and 8-10 parts of fructus alpiniae oxyphyllae for preparation. According to the invention, the zinc element is included and complexed by amylose, the formed sugar metal complex has the characteristics of no pollution, anionic property and the like, and is non-toxic in a physiological PH range, and the metal ions can be effectively utilized by a human body, so that the zinc element is intensified and supplemented in the infant rice flour.

The present invention provides a red light organic electrophosphorescence material metal iridium coordination compound, which has a structure formula represented by a formula (1), wherein R is hydrogen or methyl. The preparation method comprises that: a Suzuki coupling reaction is performed to prepare a ring metal ligand, the ring metal ligand and IrCl3.3H2O are subjected to a polymerization reaction in a 2-ethoxyethanol and water mixed solvent to obtain a chloro-bridge dimer, and the chloro-bridge dimer and the ring metal ligand are subjected to a ligand exchange reaction to obtain the red light organic electrophosphorescence material metal iridium coordination compound. According to the present invention, 1-phenoxy-4-phenyl phthalazine or a derivative thereof is adopted as the ring metal ligand to synthesize to obtain the material, wherein the emission wavelength can be adjusted so as to obtain the red light near the standard red color; and the organic electroluminescent device prepared from the material can emit high purity red light, the luminescence efficiency is high, and the preparation method is simple and is suitable for industrial production.

The invention discloses a carbon dioxide extraction method of garlic essential oil. The method comprises the following steps that garlic heads and garlic stems of garlics are respectively crushed andbeaten into a paste shape; then, mixing is performed to form mixed garlic paste; the characteristic that the self specific garlic ammonia enzyme of the garlics can oxidize the garlic paste into allicin under the aerobic condition is utilized, so that the mixed garlic paste is subjected to fermentation treatment so as to improve the content of effective ingredients in the garlic essential oil; then, an extracting agent is used for extracting and filtering the fermented garlic paste; filter liquid is subjected to carbon dioxide extraction and grading pressure reduction separation; the finished product of the garlic essential oil is finally obtained. In addition, the principle that ice points are different is utilized, so that the water and the extracting agent in residual juice of the mixedgarlic paste extracting liquid after the extraction are sequentially removed, so that the garlic essential oil is obtained again. When the carbon dioxide extraction method of the garlic essential oilprovided by the invention is used for extracting the garlic essential oil, the extraction rate of the garlic essential oil is effectively improved; meanwhile, the effective ingredients of the target product are reserved to the greatest degree; the industrial production is facilitated.

The invention relates to the field of refining of organic matters, and discloses a refining method of 2-nitro-4-methanesulfonylbenzoic acid. The method comprises the following steps: (1) in the presence of an alcohol solvent, making a 2-nitro-4-methanesulfonylbenzoic acid crude product contact an alkali, and carrying out first solid-liquid separation on the contacting product; (2) cooling the liquid phase obtained by the first solid-liquid separation in the step (1) to crystallize and separate out 2-nitro-4-methanesulfonylbenzoic acid; and (3) carrying out second solid-liquid separation on theproduct obtained in the step (2) to obtain refined 2-nitro-4-methanesulfonylbenzoic acid. According to the invention, the purity and the yield of 2-nitro-4-methanesulfonylbenzoic acid refined by themethod are high, and the refining method does not generate wastewater and is simple and convenient to operate.

The invention belongs to the technical field of medicine synthesis. The invention discloses a preparation method of DL-p-methylsulfonylphenylserine ester. DL-p-methylsulfonylphenylserine ester is prepared from L-p-methylsulfonylphenylserine ester or a salt thereof through reaction. Specifically, two methods are included. In a first method, L-p-methylsulfonylphenylserine ester or salt thereof is directly racemized to obtain DL-p-methylsulfonylphenylserine ester. In a second method, L-p-methylsulfonylphenylserine ester or a salt thereof is subjected to alkali liquor racemization and saponification to obtain DL-p-methylsulfonylphenylserine or a salt thereof, then a copper reagent is adopted to prepare D-p-methylsulfonylphenylserine copper, and finally esterification is performed to obtain theDL-p-methylsulfonylphenylserine ester. According to the method disclosed by the invention, an existing DL-p-methylsulfonylphenylserine ester production process is simplified, and the recovery efficiency is greatly improved; and the method solves the problem of complex racemization copper salt recovery process in the prior art, reduces pollution, and is more beneficial to industrial production.

The invention relates to a preparation method of a baloxavir intermediate, in particular to a method for efficiently synthesizing the baloxavir intermediate by taking 3-(benzyloxy)-4-oxo-4H-pyran- 2-carboxylic acid as a raw material through three steps of condensation reaction, hydrazinolysis reaction and cyclization reaction. The preparation method of the balosavir intermediate provided by the invention is a preparation method which is high in yield, low in cost, less in three wastes, high in product purity and suitable for industrialization.

The invention discloses application of a 2s-4b protein of a peanut 2s albumin component to induction of cancer cell apoptosis. The 2s-4b protein disclosed by the invention can be used for inducing the apoptosis of lung adenocarcinoma A549 cells; an apoptosis mechanism of the 2s-4b protein is researched; and the 2s-4b protein has broad application prospect.

The invention relates to the technical field of processing of pigeon eggs, and in particular discloses an extraction method of pigeon egg white ovalbumin. The extraction method of the pigeon egg whiteovalbumin comprises the following steps of: (1), separating pigeon egg white from yolk; filtering the egg white and taking egg white filtrate; (2), diluting the egg white filtrate, and then, performing salting-out reaction by adding a salting-out reagent step by step; (3), adjusting the pH value of the salted egg white filtrate, and performing secondary precipitation; and (4), separating precipitates to obtain the pigeon egg white ovalbumin. The method is a relatively mild extraction method, does not damage the activity of ovalbumin, is relatively high in extraction rate, and is beneficial toindustrial production.

The invention provides a method for synthesizing faropenem sodium. According to the method, oxalic acid chloride p-nitrobenzyl ester is used for replacing allyloxy oxalyl chloride in a conventional process, and thus the generation of polymer impurities is effectively prevented, and the raw material conversion rate and the product yield are increased; by adopting a method of catalytic hydrogenation under an acidic condition, silylation protection groups and p-nitrobenzyl ester protection groups are removed, organophosphorus impurities which are difficult to treat are effectively prevented, the separation and purification of products are facilitated, the maneuverability is improved, and the industrialized production is facilitated.

The invention discloses a novel human fibroblast growth factor 18. The amino acid sequence of the growth factor is as shown in SEQ ID NO:1. The soluble recombinant expression method of the N terminal-deleted human fibroblast growth factor 18 comprises the following steps: (1) selecting a plasmid containing a T7 promoter as an expression vector and corresponding escherichia coli as a host bacterium; (2) acquiring recombinants of cDNA corresponding to the SEQ ID NO:1 and expressed strains; and (3) performing induction, wherein the induction expression conditions of the expressed strains are as follows: a temperature of 15-30 DEG C, lactose induction or IPTG induction, and induction culture time of 4-16 hours. According to the preparation method of the N terminal-deleted human fibroblast growth factor 18 and a preparation of the N terminal-deleted human fibroblast growth factor 18, the N terminal-deleted human fibroblast growth factor 18 is used for treating osteoarthritis or articular cartilage injury.

The invention belongs to the field of preparation of organic silicon monomers, and discloses a method for preparing phenyldisilane from an organic silicon high-boiling material. The method comprises the steps of 1) rectifying the organic silicon high-boiling material, and selecting a fraction of 150-180 DEG C to obtain disilane; 2) adding an alkali metal or an alkali metal alloy into methylbenzene under the protection of an inert gas, and simultaneously adding a dispersant to prepare alkali metal sand; and 3) adding the alkali metal sand in the step 2) into a mixed solution of halogenobenzene and the disilane obtained in the step 1), and simultaneously adding a reaction aid to obtain a phenyldisilane product; or adding the halogenobenzene into the alkali metal sand prepared in the step 2), and performing reaction balance for 1-5h to obtain a phenyl alkali metal salt; adding the phenyl alkali metal salt into the disilane obtained in the step 1), and simultaneously adding the reaction aid to obtain the phenyldisilane product. The method disclosed by the invention is mild and controllable in process and simple in operation, the yield of the target product with active groups, namely the phenyldisilane is high, and the method is very favorable for industrial production.

The invention belongs to the technical field of veterinary traditional Chinese medicine preparations, and particularly discloses a baicalein metal complex prepared by an ore coagulation method and a preparation method of a preparation thereof. The method comprises the following steps: taking scutellaria baicalensis decoction pieces, carrying out warm soaking by using an ethanol solution with a certain concentration, carrying out vacuum concentration on the warm soaking liquid to obtain a concentrated solution A, and insulating for later use; taking one or more than two of mineral traditional Chinese medicines including chalcanthite, alumen, melanterite, calamine, ochre and red halloysite, and decocting to obtain a decoction B; and mixing the decoction B and the concentrated solution A according to a certain ratio, filtering to obtain a precipitate, washing the precipitate with purified water to obtain a baicalein metal complex, and respectively crushing, sieving, mixing and drying thecomplex and auxiliary materials to obtain a baicalein metal complex solid preparation. The baicalein metal complex is prepared from the traditional Chinese medicine scutellaria baicalensis according to the colloid coagulation principle of mineral traditional Chinese medicines, the content of the obtained baicalein metal complex is high, the used raw materials are traditional Chinese medicines, thesteps are simple and environmentally friendly and facilitate industrial production, the baicalein metal complex is applied to the field of veterinary medicine, and a new method and thought are provided for green breeding.

The invention discloses a preparation method of a series of anti-oxidants. The method comprises: reacting alkylphenol (2) with tert-butyl alcohol under concentrated sulfuric acid catalysis to generate2,6-di-t-butyl p-alkylphenol (1). The preparation method is simple in process, is economic and eco-friendly, and is suitable for industrial amplification requirement.