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Extraction method of pigeon egg white ovalbumin

A technology of ovalbumin and extraction method, which is applied in the direction of ovalbumin, albumin peptide, peptide preparation method, etc., can solve the problems of pigeon egg ovalbumin extraction and little research, etc., to achieve industrial production and high extraction rate Effect

Active Publication Date: 2020-12-04
兴宁市金鸽养殖专业合作社 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But existing technology is little to the extraction of pigeon egg ovalbumin and research; Therefore, providing a kind of method for extracting ovalbumin in pigeon egg white has important significance to the product development of pigeon egg

Method used

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  • Extraction method of pigeon egg white ovalbumin

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Effect test

Embodiment 1

[0027] The extraction method of embodiment 1 pigeon egg white ovalbumin

[0028] (1) separate dove egg white and egg yolk; use gauze to filter the egg white, get the egg white filtrate;

[0029] (2) The egg white filtrate is diluted 10 times with water, and then the salting-out reagent ammonium sulfate is added step by step to carry out the salting-out reaction;

[0030] (3) adjust the pH value of the egg white filtrate after salting out to 4.5, and carry out secondary precipitation for 20 minutes;

[0031] (4) Centrifuge under the condition of 6000r / min for 20min, remove the supernatant, and get the precipitate to obtain the pigeon egg white ovalbumin.

[0032] The specific method of adding the salting-out reagent ammonium sulfate step by step in the step (2) is: the first step adds the salting-out reagent ammonium sulfate to make its mass fraction in the egg white filtrate reach 43%, keep the salting-out reaction 20min; Add the salting-out reagent ammonium sulfate to make ...

Embodiment 2

[0034] The extraction method of embodiment 2 pigeon egg white ovalbumin

[0035] (1) separate dove egg white and egg yolk; use gauze to filter the egg white, get the egg white filtrate;

[0036] (2) The egg white filtrate is diluted 8 times with water, and then the salting-out reagent ammonium sulfate is added step by step to carry out the salting-out reaction;

[0037] (3) adjust the pH value of the egg white filtrate after salting out to 5.0, and carry out secondary precipitation for 20 minutes;

[0038] (4) Centrifuge under the condition of 6000r / min for 20min, remove the supernatant, and get the precipitate to obtain the pigeon egg white ovalbumin.

[0039] The specific method of adding the salting-out reagent ammonium sulfate step by step in the step (2) is: the first step adds the salting-out reagent ammonium sulfate to make its mass fraction in the egg white filtrate reach 42%, keep the salting-out reaction 25min; Add the salting-out reagent ammonium sulfate to make i...

Embodiment 3

[0041] The extraction method of embodiment 3 pigeon egg white ovalbumin

[0042] (1) separate dove egg white and egg yolk; use gauze to filter the egg white, get the egg white filtrate;

[0043] (2) The egg white filtrate is diluted 12 times with water, and then the salting-out reagent ammonium sulfate is added step by step to carry out the salting-out reaction;

[0044] (3) adjust the pH value of the egg white filtrate after salting out to 4.3, and carry out secondary precipitation for 20 minutes;

[0045] (4) Centrifuge under the condition of 6000r / min for 20min, remove the supernatant, and get the precipitate to obtain the pigeon egg white ovalbumin.

[0046] The specific method of adding the salting-out reagent ammonium sulfate step by step in the step (2) is: the first step adds the salting-out reagent ammonium sulfate to make its mass fraction in the egg white filtrate reach 45%, and keeps the salting-out reaction 15min; Add the salting-out reagent ammonium sulfate to ...

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Abstract

The invention relates to the technical field of processing of pigeon eggs, and in particular discloses an extraction method of pigeon egg white ovalbumin. The extraction method of the pigeon egg whiteovalbumin comprises the following steps of: (1), separating pigeon egg white from yolk; filtering the egg white and taking egg white filtrate; (2), diluting the egg white filtrate, and then, performing salting-out reaction by adding a salting-out reagent step by step; (3), adjusting the pH value of the salted egg white filtrate, and performing secondary precipitation; and (4), separating precipitates to obtain the pigeon egg white ovalbumin. The method is a relatively mild extraction method, does not damage the activity of ovalbumin, is relatively high in extraction rate, and is beneficial toindustrial production.

Description

technical field [0001] The invention relates to the technical field of pigeon egg processing, in particular to a method for extracting pigeon egg white ovalbumin. Background technique [0002] Ovalbumin is one of the active ingredients with a higher content in pigeon egg white. Ovalbumin is biologically very close to human albumin, and it is a substitute for human albumin to be developed. Ovalbumin is hydrolyzed by processing enzymes into low-molecular active peptides with biological functions such as anti-oxidation. [0003] Although there are many protein extraction processes, different processes are selected due to the selection of materials and the type of protein to be extracted, and the extraction effect is different due to different extraction processes and conditions. Among poultry eggs, eggs are relatively mature in terms of egg protein extraction technology because of their low cost and high output. In the early years, because the meat pigeon industry was general...

Claims

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Application Information

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IPC IPC(8): C07K14/77C07K1/30C07K1/34
CPCC07K14/77
Inventor 王梓颖陈伟波杨娟陈天来刘宇珍曾晓房白卫东赵文红
Owner 兴宁市金鸽养殖专业合作社
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