Herba taraxaci composite enzyme as well as preparation method and application thereof

A technology of compound enzyme and dandelion, applied in application, pharmaceutical formulation, medical preparations containing active ingredients, etc., can solve the problems of long fermentation time, unfavorable industrial production, and insignificant bacteriostatic effect, and achieves the benefits of industrial production and fermentation. Time-reduced, enhanced bacteriostatic effect

Active Publication Date: 2018-06-22
MIANYANG TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the one hand, the fermentation time of the above-mentioned patent documents is too long, which is not conducive to industrial pr

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  • Herba taraxaci composite enzyme as well as preparation method and application thereof
  • Herba taraxaci composite enzyme as well as preparation method and application thereof

Examples

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preparation example Construction

[0027] The preparation method of the dandelion compound enzyme of the present invention comprises the following steps: (1) crushing and dispersing step; (2) enzymolysis step; (3) medium preparation step; (4) fermentation step: (5) separation step. Optionally, (6) a concentration and drying step may also be included. Details are given below.

[0028] In the pulverizing and dispersing step of the present invention, the raw materials including dandelion and Houttuynia cordata are pulverized, and 40-50 parts by weight of dandelion and 50-60 parts by weight of Houttuynia cordata are respectively mixed with water to obtain a cloudy liquid. The raw materials such as dandelion and houttuynia can be pulverized separately, weighed, and then mixed with water; or the raw materials such as dandelion and houttuynia can be weighed first, then pulverized together, and then mixed with water. The raw material drug can be pulverized to be below 20 mesh, preferably below 50 mesh, more preferably...

Embodiment 1

[0052] Embodiment 1-compound enzyme

[0053] 1) Dandelion is crushed through Pharmacopoeia No. 3 sieve (50 mesh), and Houttuynia cordata is crushed through Pharmacopoeia No. 3 sieve; then 50 parts by weight of Dandelion and 50 parts by weight of Houttuynia cordata are mixed, and then added equivalent to the total amount of Dandelion and Houttuynia cordata. 10 times the amount of pure water by weight will make a cloudy solution.

[0054] 2) Combine the turbid liquid with 0.1wt% pectinase (enzyme activity is 500,000 U / g), 0.1wt% cellulase (enzyme activity is 500,000 U / g) based on the total weight of the turbid liquid, 0.1wt% Xylanase (enzyme activity: 500,000 U / g) and 0.1wt% trypsin (500,000 U / g) were mixed, and enzymatically hydrolyzed for 3.5 hours at a pH value of 6.5 and a temperature of 55°C to obtain Enzyme solution.

[0055] 3) Add 0.3wt% yeast extract, 1.5wt% ammonium sulfate, 0.05wt% zinc chloride, 0.05wt% magnesium sulfate and 0.4wt% potassium dihydrogen phosphate t...

Embodiment 2

[0058] Embodiment 2-disinfectant

[0059] The fermentation stock solution of Example 1 was mixed with water according to a weight ratio of 1:10 to obtain a disinfectant.

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Abstract

The invention discloses herba taraxaci composite enzyme as well as a preparation method and an application thereof. The preparation method comprises the steps as follows: (1) raw materials including herba taraxaci and herba houttuyniae are crushed, 40-50 parts by weight of herba taraxaci and 50-60 parts by weight of herba houttuyniae are taken respectively and mixed with water, and a suspension isobtained; (2) the suspension is subjected to enzymelysis with pectinase, cellulase, xylanase and trypsin, and an enzymatic hydrolysate is obtained; (3) nutrient substances are added to the enzymatichydrolysate, sterilization is performed, and a culture medium is obtained; (4) the culture medium is inoculated with saccharomyces for anaerobic fermentation culture for 18-30 h and then inoculated with lactic acid bacteria for still standing fermentation culture for 30-120 h, and a cultured product is obtained; (5) the cultured product is subjected to solid-liquid separation, and the composite enzyme is obtained. The fermentation time can be shortened with the method and the obtained composite enzyme has the more significant antibacterial effect.

Description

technical field [0001] The invention relates to a compound enzyme and its preparation method and application, especially a dandelion compound enzyme and its preparation method and application. Background technique [0002] Bacterial infection is an acute systemic infection caused by pathogenic bacteria or opportunistic pathogenic bacteria invading the blood circulation to grow and reproduce, producing toxins and other metabolites; clinically, it is characterized by chills, high fever, rash, arthralgia, and hepatosplenomegaly; Some may have septic shock and migratory lesions. Pathogenic microorganisms invade the blood from wounds or infected lesions in the body, causing acute systemic infection. Clinically, some patients may also experience irritability, cold extremities and cyanosis, thin and rapid pulse, rapid breathing, and decreased blood pressure. [0003] At present, there are many traditional Chinese medicines and health foods with antibacterial function, most of whi...

Claims

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Application Information

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IPC IPC(8): A61K36/78A61P31/04A23L33/00A61K36/288
CPCA23V2002/00A61K36/288A61K36/78A61K2236/19A23L33/00A23V2200/30A61K2300/00
Inventor 姜立春田徽阮期平
Owner MIANYANG TEACHERS COLLEGE
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