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Stereo multi-dimensinal multi-mode capillary electrophoresis method and its special equipment

A technology of capillary electrophoresis and special devices, which is applied in the direction of measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems of relatively harsh instrument conditions, large loss of column efficiency, and limited application, so as to avoid the reduction of column efficiency, The effect of small loss of column efficiency and guaranteed high efficiency

Inactive Publication Date: 2003-02-05
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are based on inter-column electro-mechanical switching to realize the sample from one capillary column to another capillary column. Because there are pumps, valves and other components in the flow path, although the peak capacity can be improved to a certain extent, the loss of column due to the switching process In addition, in two-dimensional gas chromatography, the second-dimensional column must be a short column, which also has a great impact on column efficiency.

Method used

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  • Stereo multi-dimensinal multi-mode capillary electrophoresis method and its special equipment
  • Stereo multi-dimensinal multi-mode capillary electrophoresis method and its special equipment
  • Stereo multi-dimensinal multi-mode capillary electrophoresis method and its special equipment

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Two-dimensional tandem capillary column electrophoresis:

[0024] Such as figure 2 As shown, capillary column A1 adopts capillary gel electrophoresis separation mode, and capillary column B2 adopts capillary electrochromatography or capillary zone electrophoresis separation mode. The required capillary columns A1 and B2 are designed to have the same size, and are respectively inserted into the interface of connector 5, and After adhesive bonding, the connecting piece 5 is a four-way structure, and the hollow capillary 4 is joined at the point-symmetrical position of the connecting piece 5. In order to avoid unnecessary loss of column efficiency, the setting of the capillary columns A1 and B2 connected through the connecting piece 5 , the sample components in the capillary column A1 should not pass through the flow path with a small angle during the process of switching into the capillary column B2, so as to avoid unnecessary peak broadening; As for the window, the det...

Embodiment 2

[0040] The difference from Example 1 is that in this example, the capillary columns A1 and B2 adopt the same separation mode of capillary gel electrophoresis, and the inner diameters of the capillary columns are different, and a matching connector 5 is used. Driven by an electric field, Auxiliary application of appropriate pressure so that the sample does not flow out of the free capillary column port, separation, that is, the overall system has great selectivity adjustment flexibility.

Embodiment 3

[0042] The difference from Example 2 is that the mobile phases of the present invention are different according to the separation mode and the actual separation needs. In addition to considering its compatibility, each mobile phase can be adjusted independently without being affected. When the capillary columns A1 and B2 When the separation mode is the same, the separation results of different mobile phases are similar to the special step gradient elution separation, and the length and height of the steps can be easily changed, which is conducive to the optimization of separation conditions.

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Abstract

The invention relates to the capillary electrophoresis method in stereo multiple dimensions and multiple modes as well as its special equipment. Switching voltage makes the mobile phase be switched between different flow paths. The sample components are separated into at least two capillary columns by using different separation modes of the capillary electrophoresis through flow paths in forms of series connection, parallel connection or their combination. The special equipment is mainly composed of at least two caillary columns, at least one connecting piece and high voltage source. The connecting piece transports the samples between columns. The container containing flow path is installed on the capillary columns or the free end of the vacant capillary.

Description

technical field [0001] The invention relates to the separation technology of capillary electrophoresis, in particular to a three-dimensional, multi-dimensional and multi-mode capillary electrophoresis method and a special device. Background technique [0002] Capillary electrophoresis is a micro-separation technique developed in recent years. Due to its high efficiency, less sample consumption, and wide application range, it has attracted widespread attention in the field of analytical chemistry. At present, it has developed into a new discipline including capillary zone electrophoresis (CZE), capillary electrokinetic chromatography (MEKC), capillary electrochromatography (CEC) and other separation modes, and dozens of commercial instruments. [0003] Although the capillary electrophoresis method has a high column efficiency, due to the limitation of the applied voltage, the length of the separation column used is restricted, thus also limiting the actual column efficiency a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N30/02
Inventor 张玉奎张维冰张庆合平贵臣
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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