Chromatographic system with double or multiple series columns

Abstract

The invention relates to a chromatographic system with double or multiple series columns. The chromatographic system comprises a liquid delivery system, a separation system, a detection system and a collecting system which are sequentially connected with one another. The liquid delivery system comprises a liquid delivery pump and a pressure measuring apparatus, the detection system comprises a UV (ultraviolet) detector, a PH (potential of hydrogen) detector and an electric conductivity detector, the collecting system comprises a collecting valve and a collector, the separation system comprises two or more than two separation units which are serially connected with one another, each separation unit comprises switching valves and a chromatographic column, the chromatographic column and the switching valves of each separation unit are connected with one another, the various separation units are serially connected with one another by pipelines, and the chromatographic columns of the various separation units are connected with one another or are disconnected from one another under the control of the switching valves of the various separation units. The chromatographic system with the double or multiple series columns has the advantages that to-be-analyzed components in a complicated sample can be roughly and precisely separated from one another by the chromatographic system, and the chromatographic system is high in separation capacity, selectivity, peak capacity, resolution and applicability.

Description

technical field [0001] The invention relates to chromatographic systems in industries such as chemical industry, pharmacy, and natural product purification, and in particular to a chromatographic system with double columns or multiple columns connected in series. Background technique [0002] In column chromatographic separation, high column efficiency is an important indicator to ensure that the sample has a certain degree of separation. In separation, the method to improve column efficiency is mainly to use small particles of chromatographic separation packing or extend the length of the separation column. Among them, the establishment of analytical methods using long chromatographic columns is the most direct and simplest means to improve absolute column efficiency. However, on the one hand, this technique is difficult to pack the chromatographic column, and on the other hand, it is usually difficult to obtain good analytical results due to the high back pressure of the c...

AI Technical Summary

Problems solved by technology

However, this technique is difficult to pack the chromatographic column on the one hand, and on the other hand, it is usually difficult to obtain good analytical results due to the high back pressure of the chromatographic column.

In the separation of natural products, polypeptides, and proteins, multiple separation methods are often adopted. First, a chromatographic column is used to enrich the sample, and after the enrichment is complete, another chromatographic column is used to refine the sample. The separation column prepares and separates the sample, the process is more complicated, and it also increases the workload, and the sample loss is also large

Images