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A secondary metabolic product chromatography and mass spectrum of naked flowers and purple beads of naked flowers and purple roads

A technology of secondary metabolites, purple pearl authenticity, applied in the field of characterization of secondary metabolites chromatography and mass spectrometry fingerprints, can solve the confusion in the application of nude flower purple pearl base sources, the same thing with different names, the same name and different things, and authentic Problems such as inability to characterize medicinal materials, achieve good reproducibility and stability, large peak capacity, and simple operation

Active Publication Date: 2022-08-09
JIANGXI POZIN PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The technical problem to be solved by the present invention is to provide a kind of secondary metabolism of naked flower purple pearl authentic medicinal materials in view of the chaotic application of naked flower purple pearl base sources in the market, the same thing with different names, the same name and different substances, and the authentic medicinal materials that cannot be characterized. Characterization Method of Product Chromatography and Mass Spectrometry Fingerprint

Method used

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  • A secondary metabolic product chromatography and mass spectrum of naked flowers and purple beads of naked flowers and purple roads
  • A secondary metabolic product chromatography and mass spectrum of naked flowers and purple beads of naked flowers and purple roads
  • A secondary metabolic product chromatography and mass spectrum of naked flowers and purple beads of naked flowers and purple roads

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Take a sample of Nasturtium purpurea (the origin of Baisha, harvest time in February 2017), pulverize, pass through a 40-mesh sieve, accurately weigh about 0.5 g of the sample powder, place it in a conical flask with a stopper, and add 25 mL of 50% methanol aqueous solution , weighed, sonicated for 30min, let cool, supplemented the weight loss with 50% methanol, shaken well, passed through a 0.22μm microporous membrane, and took the continuous filtrate as the test solution.

[0039] UPLC in Ultra Performance Liquid Chromatography (UPLC) Waters ACQUITY ARC TM Ultra-high performance liquid chromatograph; EclipsePlusC 18 RRHD chromatographic column, its specification is outer diameter 2.1mm×column length 150mm, packing diameter 1.8μm. The mobile phase is (A) 0.1% phosphoric acid aqueous solution and (B) acetonitrile, gradient elution, changing the gradient, optimizing the conditions for the fingerprint of violet bead, and selecting the spectrum with better results, 1-6 d...

Embodiment 2

[0054] Take a sample of Nasturtium purpurea (the place of origin, Baisha, harvested on October 15, 2016), pulverize, pass through a 40-mesh sieve, accurately weigh about 0.5 g of the sample powder, place it in a stoppered conical flask, add 50% methanol Aqueous solution 25mL, weighed, sonicated for 30min, let cool, supplemented the weight loss with 50% methanol, shaken well, passed through a 0.22μm microporous membrane, and took the subsequent filtrate as the test solution.

[0055] UPLC in Ultra Performance Liquid Chromatography (UPLC) Waters ACQUITY ARC TM Ultra-performance liquid chromatograph; phenomenex kintex XB-C 18 100A chromatographic column, its specification is outer diameter 2.1mm×column length 150mm, packing diameter 1.7μm. The mobile phase is (A) 0.1% phosphoric acid aqueous solution and (B) acetonitrile, gradient elution, changing the gradient, and optimizing the conditions for the fingerprint of violet bead. 1-7 different gradient elution as follows:

[005...

Embodiment 3

[0073] Take a sample of Nasturtium purpurea (the place of origin, Baisha, harvested on October 15, 2016), pulverize, pass through a 40-mesh sieve, accurately weigh about 0.5 g of the sample powder, place it in a stoppered conical flask, add 80% methanol Aqueous solution 25mL, weighed, sonicated for 45min, allowed to cool, supplemented with 80% methanol for weight loss, shaken well, passed through a 0.22μm microporous membrane, and took the subsequent filtrate as the test solution.

[0074] Using Ultra Performance Liquid Chromatography (UPLC) Waters ACQUITY ARC TM type ultra-performance liquid chromatograph; Phenomenex kintex XB-C 18 The chromatographic column has a specification of an outer diameter of 2.1 mm × a column length of 150 mm, and a packing diameter of 1.7 μm. The mobile phases were (A) 0.1% phosphoric acid in water and (B) acetonitrile, and the gradient elution was as follows:

[0075] time Mobile phase (v / v%) B time Mobile phase (v / v%) B 0mi...

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Abstract

The invention relates to the technical field of traditional Chinese medicine drug analysis, in particular to a method for characterizing secondary metabolite chromatography and mass spectrometry fingerprints of a medicinal material of Radix Nutria. The present invention adopts UPLC to establish the fingerprint spectrum of the whole components of the medicinal material of Nasturtica chinensis, and adopts the combined technology of UPLC and DAD / QTOF-MS / MS to perform the online rapid component qualitative analysis of the fingerprint spectrum of the whole components of the medicinal material of Rhododendron chinensis and its compounds. Identify and form the best phenotype of the chemical substances in the specific natural environment of the natural medicinal material. The beneficial effects of the invention are as follows: the fingerprint spectrum method of the whole components of the medicinal material of Radix japonica is simple to operate, the peak capacity is large, and the precision, reproducibility and stability are all good, the chromatographic peak separation is good, the analysis time is short, and the analysis time is short. The efficiency and sensitivity are high; the best phenotype of the chemical substances in the specific natural environment of the natural medicinal material is formed, which solves the problems of confusion in the application of the base source of the naked flower and the inability to characterize the authentic medicinal material in the market.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine drug analysis, in particular to a method for characterizing secondary metabolite chromatography and mass spectrometry fingerprints of a medicinal material of Viola nudiflora. Background technique [0002] Authentic medicinal materials are the essence of traditional Chinese medicine, which is synonymous with high-quality medicinal materials in traditional Chinese medicine. The formation of authentic medicinal materials is inseparable from the excellent germplasm of traditional Chinese medicine and the specific natural environment. For this reason, many authentic medicinal materials are often named with place names before the names of the medicines to show their authentic regions, such as Xining rhubarb, Ningxia wolfberry, Chuan Fritillaria, etc. [0003] Modern biology believes that the biological essence of Daodi medicinal materials is phenotypic variation=genetic variation+e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16H70/40
CPCG16H70/40
Inventor 杨扬李小锋杨义芳陈梁邓杰仁康兴东章弘扬夏淑英任昭洁肖绍川
Owner JIANGXI POZIN PHARMA
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