Multi-component quality control method for callicarpa nudiflora

A quality control method, the technology of naked flower purple pearl, applied in the direction of measuring device, material separation, material analysis, etc., can solve the problems such as the complex composition of naked flower purple pearl traditional Chinese medicine, which is not enough to fully control the quality of naked flower purple pearl medicinal materials, and achieve Good precision and reproducibility, simple operation, good separation effect

Inactive Publication Date: 2019-12-03
JIANGXI POZIN PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The technical problem to be solved by the present invention is to solve the technical problem that the composition of the traditional Chinese medicine of naked flower purple pearl is extremely complex, and only relying on the identification of properties and the determination of the content of several index components is not enough to fully control the quality of the naked flower purple pearl medicinal material, and provides a naked flower purple pearl Multi-component quality control method of Huazizhu medicinal material

Method used

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  • Multi-component quality control method for callicarpa nudiflora
  • Multi-component quality control method for callicarpa nudiflora
  • Multi-component quality control method for callicarpa nudiflora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take a sample of Auranthus nudiflora and pulverize it, pass through a 40-mesh sieve, accurately weigh 0.5 g of the sample powder, 7 parts in total, place them in conical flasks with stoppers, add pure water, 20% methanol aqueous solution, 40% methanol aqueous solution, 50% methanol water solution, 60% methanol water solution, 80% methanol water solution, 25mL of pure methanol, weighed, ultrasonicated for 45min, allowed to cool to 100%, and the corresponding solvent was used to make up for weight loss, shake well, pass through a 0.22μm microporous membrane, and take the continuation The filtrate was used as the test solution. Using Agilent 1200Infinity high resolution fast liquid chromatography; Agilent Eclipse Plus-C 18 The RRHD column has an outer diameter of 2.1 mm×column length of 150 mm, and a packing diameter of 1.8 μm. The mobile phase is (A) 0.1% aqueous formic acid and (B) acetonitrile, and the gradient elution is as follows:

[0033] time (minutes) ...

Embodiment 2

[0037] Take a sample of Nadiflora purple pearl and crush it, pass it through a 40-mesh sieve, accurately weigh 0.5g of the sample powder, a total of 6 parts, place them in a stoppered conical flask, add 25mL of 80% methanol aqueous solution, weigh, and weigh the 6 parts of the sample respectively. Sonicate for 15min, 30min, 45min, 60min, 75min, 90min, let cool, make up for the weight loss with 80% methanol, shake well, pass through a 0.22μm microporous membrane, and take the subsequent filtrate as the test solution. Using Agilent 1200Infinity high resolution fast liquid chromatography; Agilent Eclipse Plus-C 18 The RRHD column has an outer diameter of 2.1 mm×column length of 150 mm, and a packing diameter of 1.8 μm. The mobile phase is (A) 0.1% aqueous formic acid and (B) acetonitrile, and the gradient elution is as follows:

[0038] time (minutes) Mobile phase (v / v%) time (minutes) Mobile phase (v / v%) 0~5min 8％～14％B 30~40min 22％～30％B 5~15min 14～1...

Embodiment 3

[0042] Take a sample of Auranthus nudiflora and crush it, pass it through a 40-mesh sieve, accurately weigh 0.5 g of the sample powder, a total of 6 parts, place them in conical flasks with stoppers, add 10 mL of 80% methanol aqueous solution, 25 mL, 40 mL, 50 mL, Weigh 65mL and 75mL, sonicate for 45min, let cool, make up for weight loss with 80% methanol, shake well, pass through a 0.22μm microporous membrane, and take the subsequent filtrate as the test solution. Using Agilent 1200Infinity high resolution fast liquid chromatography; Agilent Eclipse Plus-C18 The RRHD column has an outer diameter of 2.1 mm×column length of 150 mm, and a packing diameter of 1.8 μm. The mobile phase is (A) 0.1% aqueous formic acid and (B) acetonitrile, and the gradient elution is as follows:

[0043] time (minutes) Mobile phase (v / v%) time (minutes) Mobile phase (v / v%) 0~5min 8％～14％B 30~40min 22％～30％B 5~15min 14～18％B 40~45min 30％～50％B 20~30min 18％～22％B 45~...

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Abstract

The invention relates to the technical field of pharmaceutical analysis, in particular to a multi-component quality control method for callicarpa nudiflora. The method comprises the steps of performing quality control on cinnamic acid and flavonoids components in callicarpa nudiflora by use of an ultra-high performance liquid chromatography; and analyzing and identifying the cinnamic acid and flavonoids components through a diode array detector, and detecting the ratio of peak areas of the cinnamic acid and flavonoids components to the total peak area. The measurement method provided by the invention is simple to operate, good in stability, precision and reproducibility, and the method is good in chromatographic peak resolution of callicarpa nudiflora, short in analysis time and high in analysis efficiency and sensitivity so that the material quality control of callicarpa nudiflora is more comprehensive and scientific. The cinnamic acid active compound and the flavonoids active compound are online identified by use of the diode array detector, and a new method for rapid evaluation of the medicinal material quality of callicarpa nudiflora is provided.

Description

technical field [0001] The invention relates to the technical field of analysis of traditional Chinese medicines, in particular to a multi-component quality control method of Auranthus nudiflora. Background technique [0002] Nude flower purple pearl is the dry leaves and leafy twigs of the Verbenaceae purple pearl genus. It is a commonly used traditional ethnic medicinal material in Hainan. For bruises, swelling and pain, rheumatism, tuberculosis, hemoptysis, gastrointestinal bleeding and other diseases. [0003] The chemical components of Auranthus nudica mainly include phenylpropanoids, flavonoids, triterpenoids, diterpenoids, iridoids, phenolic acids and their glycosides and sterols, etc., which mainly have hemostatic, anti-inflammatory and other pharmacological effects. It is known that the structures of phenylpropanoids in Auranthus nudica are all glycosides condensed with phenylacrylic acid and sugar or free phenylacrylic acid, caffeic acid and ferulic acid. More th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/34G01N30/64
CPCG01N30/06G01N30/34G01N30/64G01N2030/065
Inventor 杨扬康兴东杨义芳李小锋邓杰仁陈梁章弘扬夏淑英任昭洁张金良
Owner JIANGXI POZIN PHARMA
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