A method for identifying metabolites of phoxim in different tissues of freshwater fish
A technology for metabolites, phoxim, is applied in the field of aquaculture technology and detection and analysis to achieve the effects of expanding peak capacity, improving sensitivity and reliability, and reducing co-elution phenomenon.
Active Publication Date: 2022-02-18
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Even if the high-resolution (0.1u) tandem quadrupole mass spectrometer has a resolution of 1000 at a low mass-to-nucleus ratio (m / z 100) and a resolution of 10,000 at a high mass-to-nucleus ratio (m / z 1000) (FWHM) The measured mass-to-nucleus ratio (m / z) of the target can only be accurate to 1 decimal place
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Embodiment 1
[0044] 1 Test materials and methods
[0045] 1.1 Test drugs, test fish and breeding conditions
[0046] Phoxim EC (content ≥ 98%, provided by Xingtai Pesticide Co., Ltd.), acetone (excellent grade, Sinopharm Chemical Reagent Co., Ltd.), phoxim reference substance (content ≥ 98%, Dr.Ehrenstorfer Company, Germany, CAS No.: 14816-18-3), O, O, O', O',-tetraethyl phosphoryl dithioate (content ≥ 94.3%, Advanced ChemTech, USA, CAS No.: 3689-24-5), O, O-diethylthiophosphoric acid (content ≥ 98.0%, American Advanced ChemTech Company, CAS No.: 5871017-0), octinoxon (content ≥ 96.7%, German Dr. Ehrenstorfer Company, CAS No. : 14816-17 -2).
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The invention discloses a method for identifying metabolites of phoxim in different tissues of freshwater fish. The drug is administered by soaking, and qualitatively detected by ultra-high performance liquid chromatography in series with quadrupole time-of-flight high-resolution mass spectrometry (UPLC‑QTOFMS). Metabolites of phoxim in different tissues, including the following steps: (1) Freshwater fish were soaked in phoxim solution to collect different tissues; (2) Extract samples, the extraction agent was acetonitrile and ethyl acetate at a volume ratio of 5: 1 mixed, the purification agent is mixed with anhydrous magnesium sulfate and octadecylsilane bonded silica gel at a mass ratio of 10:1; (3) UPLC‑QTOFMS qualitative analysis: with phoxim, O, O, O ’ ,O ’ ,-Tetraethyl phosphorodithioate and O,O-diethyl phosphorothioate retention time and ion mass were compared to determine the metabolites of each tissue sample.
Description
technical field [0001] The invention belongs to the field of aquaculture technology and detection and analysis technology, and specifically relates to a method for qualitatively analyzing phoxim and its metabolites in freshwater fish tissues based on ultra-high performance liquid chromatography in series quadrupole time-of-flight high-resolution mass spectrometry. Background technique [0002] Phoxim is an organophosphate insecticide currently used as a veterinary drug to control mites, lice and other ectoparasites in pigs and sheep. In my country, phoxim has also been approved for killing or repelling Chinese bream, tilapia, grass carp, herring, silver carp, bighead carp, carp, crucian carp and other freshwater fish such as Chinese bream, anchor head bream, Fish lice, third-generation worms, ringworms, nematodes and other parasites. If phoxim is used improperly, it may cause its residues in aquatic animal tissues, thereby endangering human health. [0003] The main degrada...
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IPC IPC(8): G01N30/02G01N30/06G01N30/60G01N30/72G01N30/86
Inventor 刘永涛艾晓辉董靖王桢月胥宁杨秋红杨移斌周顺
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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