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Method for identifying metabolites of phoxim in different tissue of freshwater fishes

A metabolite, phoxim technology, applied in the field of aquaculture technology and detection and analysis, to achieve the effect of reducing co-elution phenomenon, good compatibility and high resolution

Active Publication Date: 2019-05-28
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

Even if the high-resolution (0.1u) tandem quadrupole mass spectrometer has a resolution of 1000 at a low mass-to-nucleus ratio (m / z 100) and a resolution of 10,000 at a high mass-to-nucleus ratio (m / z 1000) (FWHM) The measured mass-to-nucleus ratio (m / z) of the target can only be accurate to 1 decimal place

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  • Method for identifying metabolites of phoxim in different tissue of freshwater fishes
  • Method for identifying metabolites of phoxim in different tissue of freshwater fishes
  • Method for identifying metabolites of phoxim in different tissue of freshwater fishes

Examples

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Embodiment 1

[0044] 1 Test materials and methods

[0045] 1.1 Test drugs, test fish and breeding conditions

[0046] Phoxim EC (content ≥ 98%, provided by Xingtai Pesticide Co., Ltd.), acetone (excellent grade, Sinopharm Chemical Reagent Co., Ltd.), phoxim reference substance (content ≥ 98%, Dr.Ehrenstorfer Company, Germany, CAS No.: 14816-18-3), O, O, O', O',-tetraethyl phosphoryl dithioate (content ≥ 94.3%, Advanced ChemTech, USA, CAS No.: 3689-24-5), O, O-diethylthiophosphoric acid (content ≥ 98.0%, American Advanced ChemTech Company, CAS No.: 5871017-0), octinoxon (content ≥ 96.7%, German Dr. Ehrenstorfer Company, CAS No. : 14816-17 -2).

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Abstract

The invention discloses a method for identifying metabolites of phoxim in different tissue of freshwater fishes. A soaking method is adopted for drug delivery, and an ultra-high performance liquid chromatography tandem quadrupole flight time high-resolution mass spectrum (UPLC-QTOFMS) is used for qualitatively detecting the metabolites of phoxim in different tissue. The method comprises the following steps that 1, different tissue is collected after the freshwater fishes are soaked in a phoxim solution; 2, samples are extracted, wherein an extracting agent is obtained by mixing acetonitrile and ethyl acetate in the volume ratio of 5:1, and a purifying agent is prepared by mixing anhydrous magnesium sulfate and octadecyl silane bonded silica gel in the mass ratio 10:1; 3, UPLC-QTOFMS quantitative analysis is carried out, wherein the metabolites of the tissue samples are judged by being compared with phoxim, O,O,O',O',-tetraethyl dithiopyrophosphate and O,O-diethyl thiophosphate in the aspects of retention time and ion mass.

Description

technical field [0001] The invention belongs to the field of aquaculture technology and detection and analysis technology, and specifically relates to a method for qualitatively analyzing phoxim and its metabolites in freshwater fish tissues based on ultra-high performance liquid chromatography in series quadrupole time-of-flight high-resolution mass spectrometry. Background technique [0002] Phoxim is an organophosphate insecticide currently used as a veterinary drug to control mites, lice and other ectoparasites in pigs and sheep. In my country, phoxim has also been approved for killing or repelling Chinese bream, tilapia, grass carp, herring, silver carp, bighead carp, carp, crucian carp and other freshwater fish such as Chinese bream, anchor head bream, Fish lice, third-generation worms, ringworms, nematodes and other parasites. If phoxim is used improperly, it may cause its residues in aquatic animal tissues, thereby endangering human health. [0003] The main degrada...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/60G01N30/72G01N30/86
Inventor 刘永涛艾晓辉董靖王桢月胥宁杨秋红杨移斌周顺
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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