Method for continuously and stably producing high-purity raphanin

A technology for stabilizing the production of radish, which is applied in the fields of fixed on/in organic carriers, organic chemistry, fermentation, etc., can solve the problems of high cost of equipment investment, inconvenient industrial production, and incomplete hydrolysis of radish, etc. Achieve good energy-saving effect, easy industrial scale-up, and improve the effect of low vitality

Active Publication Date: 2018-06-01
CHONGQING TECH & BUSINESS UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: ① high performance liquid chromatography is used as the preparation method of high-purity products, which leads to high cost of equipment investment and packing required in production, and is not conducive to large-scale production
② During the process of preparing high-purity lyophilized product of radish, pre-freezing is very difficult due to the properties of radham and the characteristics of process engineering, so that the required pre-freezing temperature is low, the pre-freezing time is long, and the energy consumption is high, which increases the production cost.
③ Most of the degreased carmine radish seed powder on the market is a product after oil extraction. During the oil extraction process, the temperature is high, resulting in the inactivation of part of myrosinase, resulting in incomplete hydrolysis of radish, low yield of radish, and great waste of resources.
④ The equipment used is expensive, the technical requirements are high, the production capacity is limited, and it is not convenient for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for continuously and stably producing radhamin, the specific method steps are as follows:

[0035] (1) Preparation of myrosinase pretreatment solution

[0036] Using commercially available crude myrosinase as raw material, according to the mass of crude myrosinase: the volume ratio of the buffer solution is 1 g: 60 mL, first add the crude myrosinase to the buffer solution, stir slowly and mix evenly , put it into an ultrasonic processor, and perform ultrasonic treatment for 10 minutes; after the ultrasonic is completed, pump it into a vacuum filter for vacuum filtration, and collect the filtrate and filter residue respectively; wherein, the buffer solution is trishydroxymethylaminomethane with a pH of 6.5 - HCl buffer solution.

[0037] (2) Preparation of myrosinase freeze-dried powder

[0038] According to the ratio of myrosinase pretreatment solution: polyethylene glycol solution with a mass fraction of 20% and a molecular weight of 600-1000: ammonium sulfa...

Embodiment 2

[0050]A kind of preparation method of Lonicerae Lonicerae Lonicerae gargle, the concrete method step is as follows:

[0051] (1) Preparation of myrosinase pretreatment solution

[0052] Using commercially available crude myrosinase as raw material, according to the mass of crude myrosinase: the volume ratio of the buffer solution is 1 g: 100mL, first add the crude myrosinase to the buffer solution, stir slowly and mix evenly, Put it into an ultrasonic processor and perform ultrasonic treatment for 12 minutes; after the ultrasonic treatment is completed, pump it into a vacuum filter for vacuum filtration, and collect the filtrate and filter residue respectively; the buffer solution is sodium dihydrogen phosphate-citric acid with pH 6.5 buffer solution.

[0053] (2) Preparation of myrosinase freeze-dried powder

[0054] According to the ratio of myrosinase pretreatment solution: polyethylene glycol solution with a molecular weight of 25% and a molecular weight of 600-1000 with...

Embodiment 3

[0066] A kind of preparation method of Lonicerae Lonicerae Lonicerae gargle, the concrete method step is as follows:

[0067] (1) Preparation of myrosinase pretreatment solution

[0068] Using commercially available crude myrosinase as raw material, according to the mass of crude myrosinase: the volume ratio of the buffer solution is 1 g: 150mL, first add the crude myrosinase to the buffer solution, stir slowly and mix evenly, Put it into an ultrasonic processor and perform ultrasonic treatment for 10 minutes; after the ultrasonic treatment is completed, pump it into a vacuum filter for vacuum filtration, and collect the filtrate and filter residue respectively; the buffer solution is sodium citrate-citric acid buffer with pH 6.5 solution.

[0069] (2) Preparation of myrosinase freeze-dried powder

[0070] According to myrosinase pretreatment solution: polyethylene glycol solution with a mass fraction of 30% and a molecular weight of 600-1000: a mass fraction of 30% ammonium...

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PUM

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Abstract

The invention belongs to the technical field of production of high-purity raphanin, and particularly relates to a method for continuously and stably producing the high-purity raphanin. According to the method, with crude myrosinase, crosslinked chitosan microspheres and radish seed meal as raw materials, the high-purity raphanin is produced through pretreatment, enzyme immobilization, reverse osmosis, centrifugal extraction, vacuum concentration and the like. The invention provides the method for continuously and stably producing the high-purity raphanin. The method has the characteristics ofsimple, stable, continuous and easily-amplified production technology, high product yield, low equipment investment cost, low production cost, high comprehensive resource utilization rate and the like. The high-purity raphanin prepared by the method provided by the invention has the characteristics of high product purity, stable performance and the like, and can be widely applied to medicines, health products, food, daily chemical products and other industries.

Description

technical field [0001] The invention belongs to the technical field of producing high-purity radishine, and in particular relates to a method for continuously and stably producing high-purity radishine. Background technique [0002] Cruciferous vegetables contain a large amount of glucosinolates and myrosinases. When people eat such vegetables, the myrosinases in the vegetables are in full contact with glucosinolates, and glucosinolates quickly generate isothiocyanates, sulfothiocyanates, and Compounds such as cyanates and nitriles. Modern studies have shown that isothiocyanate compounds have the ability to induce phase II detoxification enzyme activity, can significantly improve the ability of the human body to remove carcinogens, and have a strong activity of inhibiting the growth and proliferation of cancer cells, thus endowing cruciferous vegetables with anti-cancer and anti-cancer properties. cancer effect. [0003] Glucosinolates widely exist in the seeds, roots, ste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12N11/10C07C331/22
CPCC12N9/24C12N11/10C12P13/002C07C331/22
Inventor 张杰邵雪晴罗妮何佳林郭渝魏航
Owner CHONGQING TECH & BUSINESS UNIV
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