Anti-ovarian function recession Chinese medicinal polysaccharide extract, preparation method and application

A technology for ovarian function and polysaccharide extraction, which is applied in the direction of drug combinations and diseases, can solve the problems of clinical application limitations, and achieve the effects of enhancing medical compliance, high content, and easy post-processing

Inactive Publication Date: 2013-01-16
南京美福天然药物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]Hormone replacement therapy (HRT) is an important method in the treatment of ovarian aging-related symptoms and diseases in modern medicine, but existing studies clearly show that it has multiple Side effects and long-term carcinogenicity limit its clinical application

Method used

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  • Anti-ovarian function recession Chinese medicinal polysaccharide extract, preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh the raw material medicine according to the ratio of each composition in Table 1, mix evenly, fully dissolve with povidone 6000 aqueous solution, add ammonium citrate according to the ratio in Table 1, fully shake, stand still, separate phases, centrifuge, and remove the upper layer A precipitate was obtained from the supernatant; the obtained precipitate was washed with ethanol, acetone and ether, respectively, and dried in vacuum to obtain polysaccharides.

[0030] Table 1 The proportions of components and reagents in the extraction process

[0031] wolfberry mulberry Rehmannia glutinosa Povidone 6000 concentration Dosage of povidone 6000 Ammonium citrate / povidone 6000 mass ratio polysaccharide content Polysaccharide Extract 1 50 copies 100 copies 75 copies 10% 135 copies 0.5:1 68.39% Polysaccharide Extract 2 125 copies 160 copies 30 copies 30% 756 copies 2:1 75.63% Polysaccharide Extract 3 200 copies...

Embodiment 2

[0033] Sample 1 (conventional water extraction and alcohol precipitation method): Take 200 kg of wolfberry, 160 kg of mulberry and 120 kg of rehmannia, add 7000 kg of water, soak for 30 minutes, decoct for 1 hour for the first time, add 7000 kg of water for the second time , decoct for 1 hour, pass through a 100-mesh screen, combine the extracts, centrifuge in a tube separator (16000r / min), take the supernatant, concentrate under reduced pressure until 1ml of the extract contains 1g of crude drug, add 90% ethanol to precipitate the two times (alcohol content is 80%), the precipitated part and the alcohol-soluble part were obtained. After the precipitated part was dried, it was determined that the extraction rate of polysaccharide was 5.387%.

[0034] Sample 2 (ultrasonic method): Take 200 kg of wolfberry fruit, 160 kg of mulberry and 120 kg of rehmannia glutinosa, add 7000 kg of water to mix, and stir evenly in the mixer, then slowly flow into the container through the pipeline...

Embodiment 3

[0041] 1) Reagents and instruments

[0042] Analytical balance; 721 type spectrophotometer; constant temperature water bath; centrifuge; concentrated sulfuric acid; absolute ethanol

[0043] 2) Prepare the solution

[0044] Glucose standard solution: Accurately weigh 0.1000 g of glucose standard product dried to constant weight in a sulfuric acid desiccator, and add water to configure a 100 mg / L glucose solution.

[0045] Anthrone reagent: weigh 0.1g anthrone and dissolve in 100ml 72% sulfuric acid (prepared on the same day)

[0046] 3) Preparation of standard curve

[0047] Precisely draw 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8ml of glucose standard solution into a 10ml stoppered test tube, add water to 1.0ml, add 4.0ml of anthrone reagent, heat in a boiling water bath for 7 minutes, Take it out and put it in a cold water bath to cool, and measure the absorbance value at 620nm with a 1cm cuvette. Taking the glucose concentration as the abscissa and the absorbance value as ...

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Abstract

The invention belongs to the field of Chinese medicines and provides an anti-ovarian function recession Chinese medicinal polysaccharide extract, a preparation method and application. The method comprises the following specific steps of: uniformly mixing 50 to 200 parts of medlar, 40 to 160 parts of mulberry and 30 to 120 parts of raw rehmannia; fully dissolving a 10 to 15 percent polymer aqueous solution in an amount which is 6 to 10 times based on the weight of bulk pharmaceuticals; adding salt and fully oscillating, wherein the mass ratio of salt to polymer is (0.5-3):1; standing to split phases; performing centrifugal separation; removing supernatant liquor to obtain precipitate; and washing the precipitate by using ethanol, acetone and diethyl ether sequentially, and performing vacuum drying to obtain a polysaccharide part. The polysaccharide component can be extracted and kept as much as possible by an appropriate extraction and purification method and an appropriate extraction and purification technology in the aspect of an effective part, so that the medicinal effect of the medicine prepared according to the Chinese herbal compound prescription can be guaranteed, the curative effect is improved, the dose is reduced, and the Chinese medicinal polysaccharide extract is definite in activity and small in toxic and side effect.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to an anti-ovarian function decline traditional Chinese medicine polysaccharide extract, a preparation method and application. Background technique [0002] Ovarian function decline can lead to a series of diseases such as infertility, reproductive system, cardiovascular system, and osteoporosis. Premature ovarian failure (POF, premature ovarian failure) leads to a decline in the quality of life of patients. In addition, with the development of society and economy, more and more professional women choose to delay their childbearing time, but the optimal reproductive time window for women is also severely limited by ovarian function. Therefore, exploring the aging mechanism of ovarian function decline, the indicators for predicting ovarian function decline, and the measures to prevent premature ovarian function decline are the research hotspots and difficultie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P15/08
Inventor 韦敏马红吕晔
Owner 南京美福天然药物科技有限公司
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