Method for preparing compound with hypoglycemic function containing cornu cervi pantotrichum insulin-like active factors
An active factor and insulin-like technology, which is applied in the field of preparation of compositions containing velvet antler insulin-like active factor with hypoglycemic function, can solve the problems of low activity and low extraction rate of velvet antler insulin active factor, and achieves high activity and improved additional Economic value, simple process effect
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specific Embodiment approach 1
[0006] Specific embodiment 1: In this specific embodiment, the steps of a preparation method of a velvet antler composition with hypoglycemic function are as follows: 1. Extraction of velvet antler insulin active factor: firstly, the velvet antler is screened, and fresh red deer antler is selected, Select one-third of the apex, grind the velvet antler into powder with liquid nitrogen and stirring beads, add the velvet antler powder and hydrochloric acid with a mass concentration of 4.5-5.5% at a ratio of 1g:5mL to 1g:10mL of material to liquid solution, extracted with a strong electric field for 25 to 35 minutes to obtain an extract containing velvet antler insulin-like active factors, and finally centrifuged with a low-temperature centrifuge at a speed of 3500 to 4000r / min for 7 to 10 minutes, and the upper layer of the extract was taken, and then the ratio of solid to liquid was The ratio of 1g: 5mL~1g:10mL is that the hydrochloric acid solution that mass concentration is 4.5...
specific Embodiment approach 2
[0007] Embodiment 2: This embodiment is different from Embodiment 1 in that: in step 1, the velvet is crushed into 40 mesh powder with liquid nitrogen and stirring beads grinding method, and the number of stirring revolutions is 40-50 revolutions per minute. The loading of grinding beads is 30-40% of the grinding chamber. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0008] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step 1, the intensity of the strong electric field is 50-100 kilovolts per square centimeter, the pulse number of the high-voltage electric field is 400-500 times, and the pulse frequency is 200-300 Hz. Others are the same as in the first embodiment.
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