Efficient preparation method for acquiring phellinus igniarius polyphenols from submerged fermentation mycelia of phellinus igniarius

A Phellinus mycelium, submerged fermentation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of difficult cultivation, slow growth, and less wild Phellinus resources, and achieve multiple Strong phenolic activity, improved extraction rate, and reduced extraction cost

Inactive Publication Date: 2021-04-16
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The fruiting body of Phellinus Phellinus is perennial and grows slowly. The resources of wild Phellinus Phellinus are small and difficult to

Method used

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  • Efficient preparation method for acquiring phellinus igniarius polyphenols from submerged fermentation mycelia of phellinus igniarius

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Embodiment 1

[0025] A high-efficiency preparation method for obtaining Phellinus polyphenols from Phellinus submerged fermentation mycelia, comprising the following steps:

[0026] S1: inoculate the Phellinus strains into the Phellinus liquid medium, after the strains are inoculated, shake the material to mix the strains with the Phellinus liquid medium;

[0027] S2: Submerge the inoculated Phellinus Phellinus liquid culture medium through submerged fermentation for 11 days. After the cultivation is completed, collect the Phellinus Phellinus mycelia, at which point the Phellinus polyphenols content reaches the highest;

[0028] S3: putting the Phellinus mycelia obtained in S2 into a vacuum freeze dryer at -80°C, freeze-drying, pulverizing, and passing through a 40-mesh sieve to obtain Phellinus mycelium powder;

[0029] S4: mix the Phellinus mycelium powder obtained in S3 with a deep eutectic solvent with a water content of 30% at a mass ratio of 1:30;

[0030] S5: Extract the mixed solut...

Embodiment 2

[0035] A high-efficiency preparation method for obtaining Phellinus polyphenols from Phellinus submerged fermentation mycelia, comprising the following steps:

[0036] S1: inoculate the Phellinus strains into the Phellinus liquid medium, after the strains are inoculated, shake the material to mix the strains with the Phellinus liquid medium;

[0037] S2: Submerge the inoculated Phellinus Phellinus liquid culture medium through submerged fermentation for 11 days. After the cultivation is completed, collect the Phellinus Phellinus mycelia, at which point the Phellinus polyphenols content reaches the highest;

[0038] S3: putting the Phellinus mycelium obtained in S2 into a vacuum freeze dryer at -80°C, freeze-drying, pulverizing, and passing through a 40-mesh sieve to obtain Phellinus mycelium powder;

[0039] S4: mix the Phellinus mycelium powder obtained in S3 with a deep eutectic solvent with a moisture content of 40% at a mass ratio of 1:30;

[0040] S5: Extract the mixed s...

Embodiment 3

[0045] A high-efficiency preparation method for obtaining Phellinus polyphenols from Phellinus submerged fermentation mycelia, comprising the following steps:

[0046] S1: inoculate the Phellinus strains into the Phellinus liquid medium, after the strains are inoculated, shake the material to mix the strains with the Phellinus liquid medium;

[0047] S2: Submerge the inoculated Phellinus Phellinus liquid culture medium through submerged fermentation for 11 days. After the cultivation is completed, collect the Phellinus Phellinus mycelia, at which point the Phellinus polyphenols content reaches the highest;

[0048] S3: putting the Phellinus mycelium obtained in S2 into a vacuum freeze dryer at -80°C, freeze-drying, pulverizing, and passing through a 40-mesh sieve to obtain Phellinus mycelium powder;

[0049] S4: mix the Phellinus mycelia powder obtained in S3 with a deep eutectic solvent with a water content of 40% at a mass ratio of 1:40;

[0050] S5: Extract the mixed solut...

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Abstract

The invention relates to an efficient preparation method for for acquiring phellinus igniarius polyphenols from submerged fermentation mycelia of phellinus igniarius. The method comprises the following steps: inoculating a phellinus igniarius strain into a phellinus igniarius mycelium culture medium, carrying out submerged fermentation culture on the inoculated phellinus igniarius mycelium culture medium, freeze-drying the obtained phellinus igniarius mycelia, conducting pulverizing, and carrying out screening with a 40-mesh sieve to obtain phellinus igniarius mycelium powder; mixing the S phellinus igniarius mycelium powder with a deep eutectic solvent having a water content of 30%-50% according to a mass ratio of 1: 30 to 1: 50; extracting an S mixed solution in an ultrasonic extractor for 30-50 minutes, conducting centrifuging in an ultracentrifuge, and taking a supernatant; separating and purifying the supernatant through D101 macroporous resin to obtain a refined phellinus igniarius polyphenol mixed solution, and removing ethanol from the mixed solution through rotary evaporation to obtain refined phellinus igniarius polyphenols. According to the method, phellinus igniarius polyphenol can be more effectively extracted, the phellinus igniarius mycelia can be fully utilized, resources are not wasted, and the method is environmentally friendly.

Description

technical field [0001] The invention relates to the field of high-efficiency utilization of active components of Phellinus Phellinus, in particular to a high-efficiency preparation method for obtaining Phellinus polyphenols from Phellinus submerged fermentation mycelia. Background technique [0002] Phellinus Phellinus is a famous medicinal fungus, known as "forest gold" and "the best of fungi". It has a sweet and pungent taste and is non-toxic. Modern pharmacological studies have shown that Phellinus has various pharmacological activities such as improving immunity, anti-tumor, anti-oxidation, anti-mutation, anti-virus, lowering blood sugar, lowering blood pressure, anti-gastric ulcer, and protecting the liver. Published studies have confirmed that Phellinus polyphenols are an important class of active substances in Phellinus, which have significant effects in anti-oxidation, anti-aging, lowering blood sugar and treating diabetes. [0003] The fruiting body of Phellinus Ph...

Claims

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Application Information

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IPC IPC(8): A61K36/07C12N1/14A61P39/06A61P3/10C12R1/645
Inventor 张蕊郑娜陈慧慧杨树德李维焕盖宇鹏程显好
Owner LUDONG UNIVERSITY
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