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A method for extracting polysaccharides and flavonoids from mulberry leaves and its application in feed

A technology of flavonoids and polysaccharides, applied in animal feed, etc., can solve the problems of lack of technical maturity and reliability, cumbersome resin adsorption process, organic solvent residue, etc., and achieve the goal of reducing feed mildew, great application value, and low cost Effect

Inactive Publication Date: 2017-07-28
伏国旺
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The methods for extracting flavonoids from mulberry leaves can be classified into three categories according to domestic and foreign patent literature retrieval: namely solvent extraction method, resin adsorption method and supercritical carbon dioxide fluid extraction method. The above-mentioned methods all have shortcomings in varying degrees at present. : The product of solvent extraction method has residues of organic solvents; the process of resin adsorption method is cumbersome, the cost is also high, and it is difficult to produce on a large scale. Research
However, there are as many as 14 kinds of Chinese herbal medicine plants used in this patent, and the process is more complicated and the production cost is higher

Method used

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  • A method for extracting polysaccharides and flavonoids from mulberry leaves and its application in feed
  • A method for extracting polysaccharides and flavonoids from mulberry leaves and its application in feed

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: A kind of method of simultaneously extracting polysaccharide, flavonoid from mulberry leaf is as follows:

[0018] (1) Take 3g of dried mulberry leaves and sodium hydroxide solution with pH = 9 and mix them evenly at a ratio of 1g:15ml. Extract them under ultrasonic waves at 65°C for 10 minutes. The ultrasonic extraction power is 150W. Filtrate with gauze while hot, collect filtrate a and filter residue a respectively;

[0019] (2) repeating the ultrasonic leaching operation of step (1) on filter residue a, collecting filtrate b and filter residue b respectively, and combining filtrate a and filtrate b to obtain filtrate c;

[0020] (3) Adjust the pH of the filtrate c to 7.5 with hydrochloric acid, then concentrate it to 15ml under reduced pressure, pour it into a 100ml beaker, add 60ml of absolute ethanol, let it stand for 12-24 hours, and filter to obtain the filtrate d and filter residue d. The filter residue d was dried in a vacuum oven at 50°C for 4 ...

Embodiment 2

[0021] Embodiment 2: the method for the polysaccharide and flavonoid that extracts from mulberry leaf is used for feed additive is as follows: Polysaccharide by weight ratio: flavonoid: sorbitol: β-carotene: disodium pyrophosphate: aluminum oxide: Choline chloride=100:30:1:10:1:1:0.5 Mix polysaccharides, flavonoids, sorbitol, β-carotene, disodium pyrophosphate, aluminum oxide, and choline chloride evenly, The feed additive is obtained, and 1 kg of the feed additive is used in every 1000 kg of feed.

Embodiment 3

[0022] Embodiment 3: the method that the polysaccharide and flavonoid extracted from mulberry leaf is used for feed additive is as follows: polysaccharide by weight ratio: flavonoid: sorbitol: β-carotene: disodium pyrophosphate: aluminum oxide: Choline chloride=120:40:3:15:5:3:2 Mix polysaccharides, flavonoids, sorbitol, β-carotene, disodium pyrophosphate, aluminum oxide, and choline chloride evenly, Promptly get feed additive, use this feed additive 2kg in every 1000kg feed.

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Abstract

The invention discloses a method for simultaneously extracting polysaccharides and flavonoids from mulberry leaves. According to the method, little equipment is adopted, the reaction condition is gentle, the cost is low, the production is safe and reliable, the product purity is high, the yield is high, and the physiological and the pharmacological activity of the polysaccharides and the flavonoids from the mulberry leaves can be kept relatively well, so that the application value is relatively great,. The polysaccharides, the flavonoids, sorbitol, beta-carotene, disodium pyrophosphate and the like extracted from the mulberry leaves are combined as feed additives, so that the diameters of pork muscle fibers are reduced, the density of muscle fibers is increased, and pork is relatively tender. The inhibition effect on mildew in the feed is strong, the feed mildew condition can be reduced, and the nutrient substances in the feed are protected from being damaged.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides and flavonoids and their application in feed, in particular to a method for extracting polysaccharides and flavonoids from mulberry leaves and their application in feed. Background technique [0002] Modern scientific research shows that polyphenols in mulberry leaves can remove free radicals and improve immunity, and have health care functions such as anti-oxidation, anti-aging, anti-radiation, and anti-cancer, and have been successfully used in medicine, health care products, oils, etc. and food industry. Polysaccharides in mulberry leaves can be used to prevent and treat cardiovascular and cerebrovascular diseases, hyperlipidemia, diabetes, obesity and anti-aging. The methods for extracting flavonoids from mulberry leaves can be classified into three categories according to domestic and foreign patent literature retrieval: namely solvent extraction method, resin adsorption method and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A23K50/30
Inventor 邹志恒谢宇宋琼莉许江伟许秋华凌云韦启鹏黄江南刘林秀陈小连
Owner 伏国旺
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