Method for simultaneously extracting polysaccharide and flavone from stems and leaves of radix tetrastigme

Abstract

The invention discloses a method for simultaneously extracting polysaccharide and flavone from stems and leaves of Tetrastigma hemsleyanum. The method comprises the following steps: drying and crushing the stems and leaves of the Tetrastigma hemsleyanum to obtain dry powder of the stems and leaves of the Tetrastigma hemsleyanum; mixing the Tetrastigma hemsleyanum stem and leaf dry powder with an acidic ethanol solution to obtain a mixed solution; carrying out water bath treatment on the mixed solution, and performing centrifuging to obtain a first supernatant and a first residue; uniformly mixing the first residue with deionized water, carrying out microwave extraction, and centrifuging the extract to obtain a second supernatant and a second residue; combining and mixing the first supernatant and the second supernatant, performing rotary evaporation to remove ethanol, adding NaCl to be a saturation state, adding an ethyl acetate solution for extraction, and performing liquid separation; concentrating the supernatant under reduced pressure and performing drying to obtain tetrastigma hemsleyanum flavone, and performing dialyzing, reduced-pressure concentrating and precipitating on the subnatant to obtain tetrastigma hemsleyanum polysaccharide. According to the method, the production process is simplified, safety and reliability are achieved, the yield is high, the biological activity of polysaccharide and flavone in the Tetrastigma hemsleyanum stems and leaves is well reserved, and the economic value is high.

Description

technical field [0001] The invention relates to a method for extracting active substances from the stems and leaves of A. trifoliate, an agricultural by-product, in particular to a method for simultaneously extracting polysaccharides and flavonoids from the stems and leaves of A. trifoliate. Background technique [0002] Clover is a plant of the genus Cliffaceae in the family Vitis genus, and it is a unique rare medicinal plant in my country. Clover is mainly distributed in Zhejiang, Fujian, Guangxi, Yunnan and other places in my country, and its underground tubers are mainly used as medicine. It was selected into the new "Zhejiang Bawei" Chinese herbal medicine in February 2018. It is mainly used for the treatment of various cancers, fever, inflammation, etc., and has been included in the list of traditional Chinese medicines reimbursed at public expense in Zhejiang Province. [0003] Previous studies have found that Clover has a wide range of pharmacological activities su...

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Problems solved by technology

[0004] It has been found through research that the flavonoid content in the stem and leaf of Clover is higher than that in the root, and the antioxidant activity is stronger; the existing patents have carried out related research on the extraction of flavonoids and polysaccharides from the stem and leaf of Clover, which usually uses ethanol solution Methods such as reflux extraction and ultrasonic extraction, but the above methods cannot realize the simultaneous extraction of polysaccharides and flavonoids from the stems and leaves of Clover, resulting in waste of polysaccharides or flavonoids, and the extraction rate is low; for example:

[0005] Patent document CN104435401A discloses a method for purifying total flavonoids from the stems and leaves of Clover, specifically extracting with 50-70% ethanol under reflux, and then purifying with macroporous resin; but the extraction rate of this method is very low, and only For the purification of flavonoids, resulting in the waste of polysaccharides

[0006] Patent literature CN107778376A discloses a method for preparing polysaccharides from the stems and leaves of Clover, using hot water extraction, and obtaining a single component through a DEAE-52 ion exchange column and Sephadex column; the extraction rate of polysaccharides in this method It is also relatively low, and causes waste of flavonoids

[0007] Patent document CN107857825A discloses a method of extracting polysaccharides from the stems and leaves of Clover by hot water extraction and enzymatic hydrolysis, but does not mention the extraction of flavonoids from Clover

[0008] Patent document CN109705229A also discloses a method for extracting polysaccharides from the stems and leaves of Clover, which is extracted by heat reflux, and then purified by ion exchange columns, which also has the problem of low extraction rate, and there is no mention of Clover Extraction of flavonoids

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