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Method for extracting triterpenoids from antrodia cinnamomea

A compound and technology of triterpenoids, applied in the field of extraction of triterpenoids, can solve the problems of poor extraction effect and cumbersome operation, and achieve the effect of simple operation, effective extraction and retention of physiological activity

Inactive Publication Date: 2014-11-05
中山安荞生物科技有限公司
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  • Abstract
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Problems solved by technology

[0003] At present, the extraction methods of triterpenoids mainly include macroporous adsorption resin method and chromatographic column preparation method, but the extraction of triterpenoids by this method is easily affected by other components in Antrodia camphorata, resulting in poor extraction effect and difficult operation. cumbersome

Method used

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Examples

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Embodiment 1

[0024] A method for extracting triterpenoids from Antrodia camphorata, comprising the following steps:

[0025] (1) Weigh 10g of Antrodia camphorata powder and 10g of diatomaceous earth, mix them, add 5% sodium bicarbonate solution, ultrasonically extract 3 times, each time for 5min, filter through a 0.45um microporous membrane after extraction, and take the filtrate;

[0026] (2) Take the filtrate prepared in step (1), add it to a 50ml measuring bottle, add chloroform to set the volume to the mark, reduce the pressure and rotary evaporate, evaporate the chloroform to dry, and obtain the crude extract of triterpenoids;

[0027] (3) Take the crude extract of the triterpenoid compound obtained in step (2), add a solvent to heat and dissolve, add 80% ethanol solution, and add it to the extraction pool of the extraction instrument for extraction. The extraction temperature is 55°C, the extraction pressure is 5Mpa, the extract is filtered, and the filtrate is taken;

[0028] (4) T...

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Abstract

The invention relates to a method for extracting triterpenoids from antrodia cinnamomea. The method comprises the following steps: firstly, mixing antrodia cinnamomea powder with diatomite, adding an extracting solvent and then carrying out ultrasonic extraction and filtration; next, adding a solvent to achieve constant volume and completely volatilizing the solvent, thereby obtaining the crude extract of triterpenoids, adding a solvent to the crude extract of triterpenoids, heating until the crude extract of triterpenoids can be dissolved, adding a pressurized solvent and carrying out pressurized extraction, filtering the extract, and finally, performing vacuum rotary evaporation on the extract, thereby obtaining the refined extract of triterpenoids. The method is characterized in that the antrodia cinnamomea is pretreated before extraction, including drying and crushing ad reduced pressure evaporation, and then pressurized extraction is carried out so that the triterpenoids in the antrodia cinnamomea can be completely extracted; besides, the whole operation conditions are mild and the physiological activity of the triterpenoids can be maintained thoroughly; besides, filtration is carried out twice in the operation process, and under the filtration assisting action of the diatomite, the effective impurities can be effectively filtered out, and therefore, the extraction effect can be prevented from being affected by other ingredients in the antrodia cinnamomea.

Description

technical field [0001] The invention belongs to the biological field, and in particular relates to a method for extracting triterpenoids from Antrodia camphorata. Background technique [0002] Antrodia camphorata (Taiwanofungus camphorates), also known as camphor mushroom or Antrodia camphorata, is a rare medicinal fungus native to Taiwan Province of my country. Antrodia camphorata mainly exists in the hollow trunk of the rare tree cinnamomum kanehirae hayata in Taiwan Province of my country. Antrodia Cinnamomea has physiological activities such as anti-tumor, increasing immunity, anti-bacteria, anti-virus, anti-allergy, anti-hypertension, lowering blood sugar, lowering cholesterol, inhibiting platelet aggregation and protecting the liver, and has few adverse reactions. Therefore, Antrodia Cinnamomea is known as the king of medicines and the ruby ​​in Taiwan’s forests in Taiwan. Modern studies have shown that Antrodia camphorata physiologically active substances include ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07
Inventor 刘巴宁郑安乔
Owner 中山安荞生物科技有限公司
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