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Method for extracting moringa oleifera saponins from moringa oleifera leaf

A technology of Moringa oleifera leaves and Moringa oleifera, applied in the field of separation and purification of natural products, can solve problems such as not involving extraction, and achieve the effects of reducing the amount of impurities introduced, saving resources, and having a high extraction rate

Inactive Publication Date: 2014-08-20
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it does not involve the extraction of biologically active substances such as saponins in Moringa

Method used

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  • Method for extracting moringa oleifera saponins from moringa oleifera leaf
  • Method for extracting moringa oleifera saponins from moringa oleifera leaf
  • Method for extracting moringa oleifera saponins from moringa oleifera leaf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Take 1 kg of Moringa leaves, crush them through a 700-mesh sieve, put them into a filter frame, put them into a CBE-5L type subcritical extraction kettle, seal the extraction kettle, adjust the vacuum in the extraction kettle to 0.1Mpa, add methanol extractant, and adjust the solid-liquid The ratio is 1:20 (g:ml), the extraction temperature is adjusted to 200°C, the extraction pressure is 4.048MPa, and the extraction is carried out 3 times, each extraction is 4h, the extract is put into a solvent recovery kettle, the pressure is reduced to a vacuum of 0.1Mpa to recover the solvent, and vacuum drying , to obtain black-green extract. Add 5 times the amount (by mass) of acetone / ether (v:v=1:1) to the extract for extraction, centrifuge in a centrifuge, take the extract, depressurize to a vacuum of 0.01-0.1Mpa, concentrate, and dry Obtain a yellow extract; take the extract and put it on a D101 macroporous resin column, and use a methanol-water eluent with a volume ratio of 5...

Embodiment 2

[0038]Get Moringa leaf 0.9kg, pulverize through 100 mesh sieves, put into filter frame, drop into subcritical extraction kettle, airtight extraction kettle, adjust the vacuum in the extraction kettle to 0.06Mpa, add ethanol extractant, adjust solid-liquid ratio to be 1 :5 (g:ml), adjust the extraction temperature to 80°C, the extraction pressure to 0.108MPa, extract 2 times, extract 0.5h each time, put the extract into the solvent recovery kettle, reduce the pressure to a vacuum of 0.01Mpa to recover the solvent, and dry it in vacuum to obtain Black-green extract. Add 5 times the amount (by mass) of acetone / ether (v:v=1:1) to the extract for extraction, centrifuge in a centrifuge, take the extract, depressurize to a vacuum of 0.01-0.1Mpa, concentrate, and dry Obtain a yellow extract; take the extract and put it on an X-5 macroporous resin column, and use methanol-water and anhydrous methanol eluent with a volume ratio of 50:50 to 99.9:0.1 to elute according to the gradient fro...

Embodiment 3

[0040] Get Moringa leaf 1.3kg, crush through a 500-mesh sieve, put into a filter frame, put into a subcritical extraction kettle, seal the extraction kettle, adjust the vacuum in the extraction kettle to 0.08Mpa, add ethanol extractant, and adjust the solid-liquid ratio to 1 :10 (g:ml), adjust the extraction temperature to 100°C, the extraction pressure to 0.223MPa, extract 3 times, extract 2h each time, put the extract into the solvent recovery kettle, reduce the pressure to a vacuum of 0.1Mpa, recover the solvent, and dry in vacuum to obtain Black-green extract. Add 5 times the amount (by mass) of acetone / ether (v:v=1:1) to the extract for extraction, centrifuge in a centrifuge, take the extract, depressurize to a vacuum of 0.01-0.1Mpa, concentrate, and dry Obtain a yellow extract; put the extract on an AB-8 macroporous resin column, and elute with ethanol-water and absolute ethanol eluent with a volume ratio of 50:50 to 99.9:0.1 according to the gradient from small to large...

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Abstract

The invention relates to a method for extracting moringa oleifera saponins from moringa oleifera leaf; and the method is as follows: 1) drying the moringa oleifera leaf, filling into a filter basket, putting into a subcritical extraction kettle, sealing the extraction kettle, adding an organic extractant, heating until an extraction liquid in the supercritical extraction kettle is in a subcritical state; putting the extraction liquid into a solvent recovery tank to obtain black green extract; 2) adding acetone / ethyl ether to the extract for extraction, using a centrifuge to take the extraction liquid, drying to obtain yellow extract; and 3) loading the extract onto a macroporous resin column, using an eluent for gradient elution according to smaller-to-greater polarity of the eluent, detecting, taking a fraction with more than 97% of saponin content, using water for elution and crystallization, and then using ethanol and methanol for recrystallization for 2-4 times to obtain a saponins product with the purity of above 98%; the saponins product is less in impurity content and high in biological activity, and the subcritical fluid extraction technology has the advantages of high extraction efficiency, short operating cycle, energy conservation, saving time, and high extractant recovery rate.

Description

technical field [0001] The invention belongs to the field of separation and purification of natural products, relates to a method for extracting saponin, a biologically active substance in Moringa oleifera, relates to a subcritical extraction technology, and is a method for finely separating biologically active substances in Moringa oleifera. Background technique [0002] Moringa (Moringa oleifera Lam), belonging to the Moringa family of the cauliflower order, is also known as the drumstick tree and the immortal tree. It is native to the sub-Himalayan region of North India and Africa, and is widely distributed in India, Pakistan and other countries. Moringa is rich in nutrients. The calcium contained in Moringa is four times that of milk, potassium is three times that of bananas, iron is three times that of spinach, vitamin C is seven times that of oranges, and vitamin A is four times that of carrots. Moringa leaves and fruit pods contain a variety of minerals, vitamins and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J75/00
CPCY02P20/54
Inventor 丁辉刘骋
Owner TIANJIN UNIV
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