Preparation method and application of metformin carbon dots

Abstract

The invention discloses a preparation method and application of metformin carbon dots, and relates to the technical field of medicine preparation, the method comprises the following steps: S1, respectively dissolving metformin hydrochloride and citric acid in deionized water; s2, transferring the two mixed solutions into a high-pressure reaction kettle system; after the reaction is finished, taking out a reaction system, cooling to room temperature, and collecting to obtain a reaction solution; s3, filtering the reaction solution by adopting a filter membrane, and collecting to obtain filtrate; and S4, removing unreacted small molecular compounds from the filtrate in the step S3 by using a dialysis bag with the molecular weight cut-off of 500Da, and finally freeze-drying to obtain a metformin carbon dot freeze-dried sample. In the preparation process of the metformin carbon dots, the basic pharmacodynamic structure of functional small molecules is not damaged, and the pharmacological activity of the functional small molecules can still be reserved, so that the metformin carbon dots can promote neural stem cells to differentiate into neuronal cells, and compared with metformin, citric acid and a mixture of the metformin and the citric acid which have the same concentration in theory, the metformin carbon dots have a better neuronal differentiation promoting proportion.

Description

technical field [0001] The invention relates to the technical field of drug preparation, in particular to a preparation method and application of metformin carbon dots. Background technique [0002] Neural stem cells can be functionally integrated into neural circuits during the process of differentiation into neurons, thereby achieving functional nerve regeneration. This feature of neural stem cells is expected to become the first choice in the treatment of most neuropathy. However, the pathological microenvironment leads to a decrease in the survival rate of neural stem cells, and the low efficiency of neural stem cells differentiation into functional neurons after transplantation in vivo limits the wide application of neural stem cells in transplantation therapy. As a first-line drug for the treatment of diabetes, metformin has been proven to promote the proliferation of neural stem cells in vivo, promote neuronal differentiation and axonal growth to establish functional...

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Problems solved by technology

However, the pathological microenvironment leads to a decrease in the survival rate of neural stem cells, and the low efficiency of neural stem cells to differentiate into functional neurons after transplantation in vivo limits the wide application of neural stem cells in transplantation therapy.

Metformin, as a first-line drug for the treatment of diabetes, has been proven to promote the proliferation of neural stem cells in vivo, promote neuron differentiation and axon growth, and establish functional synaptic connections. Because small molecule drugs are easily excreted from the outside of the cell, it can be used in stem cell transplantation. The effect in the weakens with the passage of time

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