Method for preparing monodisperse polystyrene microspheres with controllable particle size

Abstract

The invention belongs to the field of biomedicine and particularly discloses a method for preparing monodisperse polystyrene microspheres with a controllable particle size. The method is characterized by comprising the following steps of: adding water and emulsifier into a three-necked flask, stirring a solution until the solution is clarified, introducing nitrogen to replace air in the reaction system, adding a certain amount of monomers, emulsifying for 0.5 to 2.5 hours, heating to reaction temperature of between 65 and 90 DEG C, stirring again, dripping an initiating agent under the protection of nitrogen, and performing polymerization reaction for 12 to 48 hours; and stopping reaction to obtain emulsion particles, performing centrifugal sedimentation by using an ultracentrifuge, discarding supernate, adding absolute ethanol, performing ultrasonic dispersion, repeating the steps for 3 to 4 times, pouring washed microspheres into culture dish, putting in a vacuum drying oven, and drying to obtain polymer microspheres. Compared with the prior art, the method for preparing the monodisperse polystyrene microspheres with the controllable particle size has the advantages that raw materials are low in cost and can be obtained easily; the process is simple; and the prepared monodisperse polystyrene microspheres with the controllable particle size can be applied to the field of biomedicine, micro electronic techniques and the like well.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a method for preparing monodisperse polystyrene microspheres with controllable particle size. Background technique [0002] With the application of monodisperse polymer microspheres in biomedicine, analytical chemistry, chromatographic technology, microelectronics and other fields, in the past few decades, researchers at home and abroad have focused on obtaining monodispersity with controllable particle size. A lot of research has been done on polymer microspheres, and many results have been obtained. [0003] For example: Some scientists use 2,2-azo (2-methylpropylamidine) dihydrochloride as an initiator, and obtain the optimum polymerization reaction through the amount of monomer, the amount of initiator, and the pH of the reaction system. good condition. Some scientists added an amphoteric surfactant, dimethyl dodecyl ammonium inner salt, to the reaction system of styrene, potassi...

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Problems solved by technology

[0005] However, the specific operation of the above methods is relatively complicated, the raw materials are expensive, and the requirements for certain instruments are relatively high. For ordinary biopharmaceutical enterprises, they cannot be popularized and used.