Velvet antler insulin active factor extraction method
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A technology of active factors and extraction methods, which can be applied in diseases, pharmaceutical formulas, allergic diseases, etc., can solve the problems of low activity and low extraction rate of red deer antler insulin active factors, achieve high activity, increase additional economic value, The effect of high extraction rate
Inactive Publication Date: 2009-05-06
NORTHEAST FORESTRY UNIVERSITY
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Problems solved by technology
[0004] In order to solve the problem of low extraction rate and low activity of red deer antler insulin active factor, the present invention provides a method for preparing deer antler insulin active factor by strong electric field method, which can effectively improve the extraction rate and product activity of red deer antler insulin active factor
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specific Embodiment approach 1
[0006] Specific embodiment one: In this specific embodiment, the steps of a method for extracting red deer antler insulin active factor are as follows: 1. Extraction of red deer antler insulin active factor: firstly carry out the screening of red deer antler, select fresh red deer antler, and select At one third of the apex, use liquid nitrogen and stirring beads to grind the red antler into powder, and add the antler powder and hydrochloric acid with a mass concentration of 4.5 to 5.5% at a ratio of 1g: 5mL to 1g: 10mL. solution, extracted with a strong electric field for 25 to 35 minutes to obtain an extract containing red deer antler insulin-like active factors, and finally centrifuged with a low-temperature centrifuge at a speed of 3500 to 4000r / min for 7 to 10 minutes, and the upper layer of the extract was taken. Add the hydrochloric acid solution with a mass concentration of 4.5 to 5.5% to the lower layer of the precipitate at a ratio of 1g: 5mL to 1g: 10mL to continue s...
specific Embodiment approach 2
[0007] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in Step 1, the red antler is crushed into a 40-mesh powder with liquid nitrogen and stirring beads, and the stirring speed is 40-50 per minute. Turn, the loading capacity of the grinding beads is 30-40% of the grinding chamber. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0008] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step 1, the intensity of the strong electric field is 50-100 kilovolts per square centimeter, the pulse number of the high-voltage electric field is 400-500 times, and the pulse frequency is 200-300 Hz. Others are the same as in the first embodiment.
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Abstract
The invention discloses an extraction method of cervus elaphus linnaeus insulin active factors and relates to a preparation method of the cervus elaphus linnaeus insulin. The invention solves the problems of low extraction rate, low activity and fussy production technique of a traditional method in extracting the cervus elaphus linnaeus insulin active factors. The technical process of the extraction method of the cervus elaphus linnaeus insulin active factors comprises the following steps of: selecting fresh two-branched velvet of a red deer; selecting the one third location of the front end, using a liquid nitrogen and a stirring bead grinding method to crack the cervus elaphus linnaeus, using a strong electric field for extraction, using a low-temperature overspeed centrifuge to separate an extract and remove grease matters, and then using a vacuum scraper type film for condensing and a vacuum freezer for frozen-drying, thus obtaining the frozen-dried powder of the cervus elaphus linnaeus insulin active factors. The cervus elaphus linnaeus insulin prepared by the method is a natural extract, does not have side effects to human bodies, has the effects of reducing blood sugar, resisting aging and fatigue, hairdressing, beautifying, protecting liver and nourishing kidney, and can be applied to the fields such as food, health products, makeup, medicines, biochemical industry, and the like.
Description
technical field [0001] The invention relates to a preparation method of red deer antler insulin. Background technique [0002] The main function of red deer antler insulin-like active factor is to promote cell differentiation, proliferation and survival, and it also has the function of insulin. It is of great significance to stimulate the synthesis of RNA and DNA and cell proliferation, especially in the C0-G1 and G1-Gs stages of the cell cycle cycle. It plays an important role in the growth of bone, muscle, fat tissue, liver, kidney, brain, adrenal gland, cardiovascular, digestion, immune system, and tumor cells; it has a huge impact on human physical strength, energy, endurance, appearance and life as a whole improve. It can regulate endocrine, enhance the body's immunity, promote the growth and metabolism of the body, and has the functions of anti-aging, anti-fatigue, beauty and beauty, liver and kidney protection and sexual function improvement. [0003] At present, t...
Claims
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