Full-automatic hybridization oven and implementation method and application thereof

Abstract

The invention provides a full-automatic hybridization oven and an implementation method and application thereof. The full-automatic hybridization oven structurally comprises a hybridization reacting box, a sample feeding device for adding a reagent to the hybridization reacting box, a reagent supply device, a reagent temperature controlling device and a control terminal, wherein, the hybridization reacting box is provided with hybridization membrane strips, the reagent supply device is sequentially connected with the reagent temperature controlling device and the sample feeding device througha pipeline, and the control terminal is connected with the reagent temperature controlling device. The hybridization process of the hybridization oven of the invention is controlled by the control terminal to realize full-automatic hybridization operation. The invention can be applied to existence and quantification of affinity mates in chemical molecular binding reactions such as nucleic acid hybridization, antibody-antigen reaction, receptor-ligand reaction, ion-culture medium reaction in a chemical binder and the like.

Description

technical field [0001] The invention relates to molecular hybridization technology, in particular to an automatic molecular hybridization instrument and its realization method and application. Background technique [0002] The process in which complementary nucleotide sequences form a stable hybrid double-stranded molecule (DNA molecule) through Wilson-Crick base pairing is called hybridization. The hybridization process is highly specific, and specific target sequence detection can be performed based on the known sequences of the probes used. Nucleic acid molecular hybridization technology has high sensitivity and high specificity, so in the field of molecular biology, this technology has been widely used in the screening of cloned genes, the production of restriction map, the qualitative and quantitative detection of specific gene sequences in the genome and disease diagnosis. In addition, it has been widely used not only in the field of molecular biology, but also in cl...

AI Technical Summary

Problems solved by technology

[0007] However, the traditional hybridization technology or reversed-phase dot hybridization technology is carried out in glass tubes, centrifuge tubes or plastic bags at an appropriate temperature.

Existing molecular hybridization instruments mainly include the following two types: one is a water bath, which is just a device for maintaining a constant temperature. The entire process of hybridization reaction requires manual operation, and the temperature control accuracy is low, and the temperature uniformity is poor. Cross-contamination is prone to occur; the other is the Cape hybridization instrument. Compared with the water bath, the Cape hybridization instrument only adds an automatic draining device. Many processes still require manual operations. It is a semi-automatic product with low temperature control accuracy and uniform temperature. Poor performance and more prone to cross-contamination

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