Parallel circulating type cell selective filter recombiner

Abstract

The invention relates to a filtration recombiner, in particular to a parallel circulation cell selective filtration recombiner. The parallel circulation cell selective filtration recombiner adopts the technical proposal that the parallel circulation cell selective filtration recombiner comprises an injection pump, a first pipe, a second pipe, a first right connecting pipe, a first left connectingpipe, a second right connecting pipe, a second left connecting pipe, a first bidirectional crack valve, a second bidirectional crack valve, a switch valve, an exhaust hole and a peristaltic pump, andalso comprises a filtration box which is provided with a filter screen and a movable cover. The parallel circulation cell selective filtration recombiner has the advantages of needlessness of antibody and antigen reaction, needlessness of using beads and other media, needlessness of in vitro culture, needlessness of special centrifugal equipment, quick speed, and utilization of the adhesivity of bone marrow stromal cells to perform bone marrow stromal cell filter-type quick screening on marrow blood.

Description

technical field [0001] The invention relates to a filter complex, in particular to a parallel circulation type cell selective filter complex. Background technique [0002] The repair of bone defects often requires bone grafting or bone substitute materials to fill, and the current research hotspot in tissue engineering in this field is the scaffold carrier compounded with stem cells. Bone marrow stromal stem cells can be isolated by adherent culture or density gradient centrifugation, and then expanded in vitro. Although bone marrow stromal stem cells can be isolated in vitro and scaffolds containing cells can be prepared, they are still in the laboratory research stage and need to be implanted. For the human body, there are still many technical and ethical problems, and the cultivation cycle is long, easy to be polluted, and the cost is high, which affects the clinical application and promotion. [0003] Currently, cell screening techniques mainly include: 1) flow cytometr...

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Problems solved by technology

[0005] 1) Although the separation method of flow cytometry separates cells quickly and has high purity, it is expensive, and it is difficult to separate trace cells with too low content, and it cannot handle a large number of cell samples, and it is also difficult to obtain a sterile cell suspension

2) The immunomagnetic bead separation method has a great impact on the activity of cells, and the recovery rate is only 60%-75%, which may lead to complete inactivation of cells. The same operation is more complicated and expensive

However, it takes a long time, involves in vitro culture of cells, and is difficult for clinical use.

4) Density gradient centrifugation is a commonly used method for concentrating and separating cells, but it often requires the use of a separation medium (such as Ficoll or Hypaque solution, etc.). Washing away the separation medium, maintaining the sterility of the entire operation requires special equipment, which is expensive

[0007] 1) Static compounding or using negative pressure technology is generally aimed at cultivating and purifying bone marrow stromal stem cells, which requires an in vitro culture process, but not applied to raw bone marrow blood. The reason is that the number of bone marrow stromal stem cells in bone marrow blood is small, and the efficiency of static compound culture is poor.

2) Mixed culture, co-cultivation of cells and scaffolds, also inseparable from the instability of compound efficiency during in vitro culture

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