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Fast detection reagent for melamine

A technology for melamine and detection reagents, applied in the field of melamine rapid detection reagents, can solve the problems of long operation time, large equipment investment and high professional requirements, and achieve the effects of convenient operation, fast detection process and low detection cost

Inactive Publication Date: 2009-04-08
QUICKING BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems are that the equipment investment is large, the operation time is long, and the professional requirements are high. It is not suitable for the requirements of rapid on-site detection and the detection of large batches of samples.

Method used

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  • Fast detection reagent for melamine
  • Fast detection reagent for melamine
  • Fast detection reagent for melamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] Example, melamine rapid detection kit.

[0014] exist figure 1 In the method, the nitrocellulose membrane (1) coated with melamine-BSA conjugate is pasted in the middle of the bottom plate (4), the filter paper (2) is pasted on the top, and the sample processing part (3) is pasted on the bottom. Colloidal gold particles labeled with an anti-melamine antibody are attached to a polyester membrane (5). The bottom plate (4) pasted with nitrocellulose membrane (1), absorbent paper (2) and glass fiber filter pad (3) is placed on the reagent case (6), and the reagent case (6) is tightly covered.

[0015] 1. Coat a certain concentration of melamine-BSA conjugate and goat anti-rabbit IgG on the position of the detection line and control line on the nitrocellulose membrane.

[0016] 2. Paste the prepared nitrocellulose membrane together with the sample processing part and filter paper on the bottom plate in sequence.

[0017] 3. Assemble the bottom plate in the bottom of the r...

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Abstract

The invention provides a melamine quick detection reagent which adopts highly specific antibody and antigen reaction and immunity-chromatographic technology to achieve the aim of quickly detecting the melamine in a sample. The reagent has the advantages of simple, convenient and quick operation and the like, can be applied to the production fields such as bread cultivation, breeding, animal husbandry, food processing and the like for quickly detecting the melamine in the food and feed in-situ and also provides simple and convenient means for the safety detection of household food.

Description

technical field [0001] Melamine rapid detection reagent, applying the principle of immunology, achieves the purpose of detecting melamine through the immune reaction of colloidal gold and then with the help of immunochromatography technology, and is applied to the rapid detection of melamine in fresh milk, dairy products, soybean products, feed, baby food and pet food . Background technique [0002] Because melamine is a kind of chemical raw material, its toxicity is mild, and the median lethal dose of oral administration to rats is greater than 3 g / kg body weight. According to an experimental report in 1945: No obvious poisoning was observed after feeding large doses of melamine to rats, rabbits and dogs. Long-term intake of melamine by animals can cause reproductive and urinary system damage, bladder and kidney stones, and can further induce bladder cancer. In 1994, the third volume of the "International Chemical Safety Manual" and the International Chemical Safety Card ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/544
Inventor 王学生冯吉顾琳杜森垚
Owner QUICKING BIOTECH
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