Kit for separating RNA (ribonucleic acid) bound in RNA binding protein
A technology for binding proteins and kits, which is applied in the field of molecular biology and can solve the problems of many influencing factors, easy pollution and low yield.
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[0022] Isolation of mRNA bound by RNA-binding protein HuR in Raw264.7 cells after LPS stimulation
[0023] 1. Reagent source:
[0024] (1) Magnetic bead A is Dynabeads of Invitrogen Company;
[0025] (2) The magnetic bead G is Dynabeads of Invitrogen Company;
[0026] (3) Mouse IgG of the present invention is produced by Sigma Company;
[0027] (4) RNase inhibitor is for the production of RNase OUT for Invitrogen;
[0028] (5) Protease mixed inhibitor produced by Roche;
[0029] (6) KCl, MgCl 2 , Hepes, surfactant Nonidet P-40, dithiothreitol, 4-hydroxyethylpiperazineethanesulfonic acid are all produced by Sigma.
[0030] 2. Instrument source:
[0031] (1) Refrigerated centrifuge, Sigma;
[0032] (2) Microplate reader, iQ5Multi color Red-time PCR Deteetion System, all are Bio-Rad;
[0033] (3) Four-dimensional rotator, Qilin Bell;
[0034] (4) Magnetic stand, Invitrogen;
[0035] (5) Gene Quent Nucleic Acid Quantitative Instrument, GE Company;
[0036] 1. Preparatio...
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