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Micro fluid control chip for immunilogical analysis and its use in immunilogical analysis

A microfluidic chip and immune analysis technology, applied in the field of immune analysis, can solve the problems of non-specific adsorption, slow response, cumbersome operation, etc., and achieve fast and convenient analysis

Inactive Publication Date: 2005-05-18
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

It has many shortcomings such as cumbersome operation, slow reaction, poor repeatability, and often accompanied by non-specific adsorption.

Method used

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  • Micro fluid control chip for immunilogical analysis and its use in immunilogical analysis
  • Micro fluid control chip for immunilogical analysis and its use in immunilogical analysis

Examples

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Embodiment 1

[0022] Taking hen ovalbumin as an example, the protein has a molecular weight of 43kDa and consists of 386 amino acids. The chips used are figure 1 The designed chip is made of quartz, and the channel size is 50 μm×20 μm. Polyacrylamide filler is used in the channel of the second unit. 100mg / mL hen's egg protein is carrying out the sample injection of the first unit, and the injection channel is 0.5 centimeters, and the separation channel is 2 centimeters, and the buffer solution that selects is 20mmol / L sodium hydroxide and 4% methylcellulose solution is Catholyte, 100mmol / L phosphoric acid is used as the anode electrolyte, the sample injection electric field is 200V / cm, the separation electric field is 260V / cm, the time takes about 60 seconds; then part of the sample enters the second unit under the action of the electric field, the second unit The main microchannel is 3 cm, the eluent transport channel is 1 cm, the eluent is 8M urea, the buffer solution is 20 mmol / L borax,...

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Abstract

The microfluid control chip for immunological analysis features that the chip consists of four basic units connected successively, with the sample outlet of the upper basic unit being connected to the sample inlet of the next basic unit. The first basic unit is sample injecting unit; the second one is antibody and antigen reaction unit with specific antibody linked to the inner wall of the micro channel loaded on the stuffing; the third one is post-column fluorescent mark unit; and the fourth one is antigen separating and detecting unit. The chip of the present invention is used in analysis of target antigen to obtain information in short time and through simple operation.

Description

Technical field: [0001] The invention relates to immune analysis technology, and in particular provides a microfluidic chip specially used for immune analysis. Background technique: [0002] Immunoassay is an analytical method in which antibodies are used as analytical reagents. Its high selectivity, low detection limit, and broad application range make it a powerful analytical tool in clinical, biopharmaceutical, and environmental chemistry. The high specificity of antibodies enables immunoassays to analyze trace analytes in samples in the presence of a large number of structural analogs. Various labeling methods, such as radioactive labels, enzyme labels, chemiluminescence labels and fluorescent labels etc., so that the immunoassay has extremely high selectivity. Therefore, immunoassay is especially suitable for the detection of low concentration analytes in complex samples such as blood and urine. [0003] The most conventional immunoassay is to immobilize the antibody...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/53G01N33/533G01N33/543G01N33/558G01N33/561
Inventor 罗勇林炳承
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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