A kind of prostatic excretory protein antigen and its antibody and application

A technology of prostatic corpuscle and protein antigen, which is applied in the field of prostatic corpuscle leaking protein antigen and its preparation, can solve the problems of inapplicability to the detection of prostatitis and the inability to distinguish samples from patients with chronic prostatitis and normal people, and achieve detection The method is simple and reliable

Active Publication Date: 2018-03-27
ONCO BIOMEDICAL TECH SUZHOU
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, this kit is only suitable for the detection of prostate cancer and cannot distinguish between samples from patients with chronic prostatitis and normal people, so it is not suitable for the detection of prostatitis

Method used

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  • A kind of prostatic excretory protein antigen and its antibody and application
  • A kind of prostatic excretory protein antigen and its antibody and application
  • A kind of prostatic excretory protein antigen and its antibody and application

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Preparation of Prostatic Excretion Protein Antigen (PSEP): Take 500 ml of mid-morning urine from patients with chronic prostatic inflammation, add the same amount of HEPES buffer (pH 7.3) and protease inhibitor (American Sigma company), and filter with gauze. Take the filtrate, place them in 10 50-ml centrifuge tubes, and centrifuge at 400g for 10 minutes at a low temperature of 4°C. Remove the precipitation and take the supernatant. Centrifuge at 10,000g for 20 minutes at a low temperature of 4°C. Remove the precipitation and take the supernatant. They were placed in 200 3ml ultracentrifuge tubes and centrifuged at 100,000g for 120 minutes at a low temperature of 4°C. Pour out the supernatant and take the precipitate. Add 2.5 ml of TBS buffer (pH 7.8) and protease inhibitor (Sigma, USA), and mix. Centrifuge at 100,000g for 120 minutes at a low temperature of 4°C. Pour out the supernatant and take the precipitate. Dissolved in 2.5 ml 0.5% NP-40, 80 mM NaCl, 0.2% De...

Embodiment 2

[0046] The composition of the kit described in this embodiment is as follows: mid-section urine of patients with prostatic inflammation; blocking solution; prostatic corpuscle protein antigen (PSEP); PSEP detection antibody; cleaning solution; color developing agent; termination solution.

[0047] The specific methods for the detection or diagnosis of prostatitis using the above kit are as follows:

[0048] 1. Specimen source: According to the diagnostic criteria of prostatitis in the "Guidebook for Diagnosis and Treatment of Urological Diseases in China" (Na Yanqun et al., 2011), 213 mid-stage urine samples of patients with prostatic inflammation were collected, and chronic diseases that were fully consistent with comprehensive clinical diagnosis 176 cases of prostatitis. Age 16-72 years old, average age 34 years old. 100 urine samples of normal human subjects, aged 20-68 years old. The average age is 34 years old. Urine routine was normal. Test the urine immediately after col...

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Abstract

The invention relates to a prostateexosomal protein (PESP) antigen, a specific corresponding antibody and application thereof, and on the basis further acquires a kit based on prostasome leakage protein quantitative indirect enzyme-linked immunoadsorption so as to effectively detect and diagnose chronic prostatitis. By means of reaction system precision detection, stability test and destructive test on the urine detection kit based on PESP antibody-antigen reaction, the reagent quality shows high batch production probability. The application is truly noninvasive, the operation is simple, and the kit has high sensitivity and specificity. The kit can alleviate the patient pain caused by chronic pelvic pain syndrome / chronic prostatitis, and also early diagnosis can further strengthen prevention and treatment of prostate cancer, therefore the kit has wide application prospect.

Description

Technical field [0001] The present invention relates to a prostatic corpuscle protein antigen, in particular to a prostatic corpuscle protein antigen and a preparation method and application thereof. Background technique [0002] Chronic Prostatitis (CP) is one of the most common diseases among young and middle-aged men in urology, and it is reported to account for 30% of outpatient visits. According to epidemiological surveys, more than 10% of adult males in my country have prostatitis-like symptoms, and 50% of men have suffered prostatitis at different times. It shows that there are many patients with potential chronic prostatitis or chronic pelvic pain (CPPS). At the same time, the inflammatory process caused by chemicals, physical factors or biological agents is also an important factor in the pathogenesis of human cancer. In prostate cancer, the existence of inflammation and the regeneration of cell epithelial tissue after injury are considered to be the key factors for ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C07K16/18G01N33/68G01N33/577
CPCG01N33/577G01N33/68
Inventor 钱泽陆群曾燕张娇
Owner ONCO BIOMEDICAL TECH SUZHOU
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