A kind of prostatic excretory protein antigen and its antibody and application
A technology of prostatic corpuscle and protein antigen, which is applied in the field of prostatic corpuscle leaking protein antigen and its preparation, can solve the problems of inapplicability to the detection of prostatitis and the inability to distinguish samples from patients with chronic prostatitis and normal people, and achieve detection The method is simple and reliable
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Embodiment 1
[0044] Preparation of Prostatic Excretion Protein Antigen (PSEP): Take 500 ml of mid-morning urine from patients with chronic prostatic inflammation, add the same amount of HEPES buffer (pH 7.3) and protease inhibitor (American Sigma company), and filter with gauze. Take the filtrate, place them in 10 50-ml centrifuge tubes, and centrifuge at 400g for 10 minutes at a low temperature of 4°C. Remove the precipitation and take the supernatant. Centrifuge at 10,000g for 20 minutes at a low temperature of 4°C. Remove the precipitation and take the supernatant. They were placed in 200 3ml ultracentrifuge tubes and centrifuged at 100,000g for 120 minutes at a low temperature of 4°C. Pour out the supernatant and take the precipitate. Add 2.5 ml of TBS buffer (pH 7.8) and protease inhibitor (Sigma, USA), and mix. Centrifuge at 100,000g for 120 minutes at a low temperature of 4°C. Pour out the supernatant and take the precipitate. Dissolved in 2.5 ml 0.5% NP-40, 80 mM NaCl, 0.2% De...
Embodiment 2
[0046] The composition of the kit described in this embodiment is as follows: mid-section urine of patients with prostatic inflammation; blocking solution; prostatic corpuscle protein antigen (PSEP); PSEP detection antibody; cleaning solution; color developing agent; termination solution.
[0047] The specific methods for the detection or diagnosis of prostatitis using the above kit are as follows:
[0048] 1. Specimen source: According to the diagnostic criteria of prostatitis in the "Guidebook for Diagnosis and Treatment of Urological Diseases in China" (Na Yanqun et al., 2011), 213 mid-stage urine samples of patients with prostatic inflammation were collected, and chronic diseases that were fully consistent with comprehensive clinical diagnosis 176 cases of prostatitis. Age 16-72 years old, average age 34 years old. 100 urine samples of normal human subjects, aged 20-68 years old. The average age is 34 years old. Urine routine was normal. Test the urine immediately after col...
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