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Kit for diethyl phthalate fluorescence polarization immunoassay

A technology of diethyl phthalate and immune detection, which is applied in the field of food inspection and immune analysis, can solve the problems of export product quality not reaching the exporting country, damage to China's image, and economic loss in export trade, etc.

Inactive Publication Date: 2012-07-18
NANKAI UNIV
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  • Application Information

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Problems solved by technology

If there is no fast and accurate detection method to check, the quality of exported products cannot meet the requirements of the exporting country, which will cause huge economic losses in my country's export trade and damage China's image in the world

Method used

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  • Kit for diethyl phthalate fluorescence polarization immunoassay
  • Kit for diethyl phthalate fluorescence polarization immunoassay
  • Kit for diethyl phthalate fluorescence polarization immunoassay

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Embodiment

[0035] A diethyl phthalate fluorescent polarization immunoassay kit, including fluorescent marker stock solution, DEP standard solution, DEP polyclonal antibody serum and concentrated phosphate (PBS) buffer solution and respectively packaged in reagent tubes, fluorescent The marker stock solution is a solution obtained by dissolving fluorescein isothiocyanate (FITC)-labeled DEP fluorescent conjugates in 200 μL methanol and packaging it in a reagent tube with a volume of 1.5 mL; DEP standard solution is as follows: DEP in methanol solvent The different concentrations of DEP were set and packaged in seven reagent tubes with a volume of 1.5mL, and the solvent was methanol, 1mL in each tube; DEP polyclonal antibody serum was 600μL and packaged in a reagent tube with a volume of 1.5mL; concentrated PBS buffer The solution is packaged in two reagent tubes with a volume of 10 mL, and each reagent tube contains 9 mL of liquid.

[0036] The preparation of described solution:

[0037] ...

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Abstract

The invention discloses a kit for diethyl phthalate fluorescence polarization immunoassay. The kit comprises a fluorescence indicator stock solution, a DEP (Diethyl Phthalate) standard solution, a DEP polyclonal antibody serum and a concentrated phosphate buffer solution (PBS), which are respectively encapsulated in a reagent tube for detecting the concentration of the DEP in a sample. According to the detection method, a fluorescence polarization technology is adopted, a standard curve is obtained through measuring the fluorescence polarization of a series of DEP solutions with the known concentrations, and the concentration of the DEP in a mixture is computed by using the standard curve. The kit, provided by the invention, has the advantages that high degree of specificity of an antibody-antigen reaction and high sensibility of a fluorescence indicator are combined by the detection method, so that the kit is simple and quick to operate, free of operations such as separation during detecting, and high in detection precision, the maximum detection range of the DEP is 10 to 200 ng / mL, the sensitivity reaches 40.4 ng / mL, and the kit plays an important role in quick detection of the residual DEP in actual systems, such as water, juice drinks and capsules.

Description

【Technical field】 [0001] The present invention relates to a food inspection immunoassay technology, in particular to a diethyl phthalate fluorescence polarization immunoassay kit. 【Background technique】 [0002] In April 2011, Taiwan's relevant departments found that "Jingyuan Probiotics" contained plasticizer di(2-ethyl)hexyl phthalate (DEHP) during routine sampling of food, and the concentration was as high as 600ppm (parts per million). one concentration). On May 23, Taiwan discovered that Yushen Fragrance Company had illegally added toxic plasticizers to the food additive "clouding agent" (that is, emulsifier), which resulted in contamination of 9 well-known sports drinks, fruit juices, and enzyme drinks. Taiwan was affected by the incident. Nearly 200 manufacturers were involved. As of June 6, 2011, the General Administration of Quality Supervision, Inspection and Quarantine announced the latest "List of Problem Enterprises and Products Polluted by Plasticizers in Tai...

Claims

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Application Information

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IPC IPC(8): G01N33/533
Inventor 郗日沫田溪孟萌尹永梅张波宋佩薛虎寅张太昌邢玥王鹏徐志环
Owner NANKAI UNIV
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