Biosensor, method for producing the same and sensor measurement system

Abstract

A biosensor capable of detecting, for example, the index of refraction, the concentration of proteins, or antibody-antigen reactions in a sample using surface plasmon resonance are provided, as well as methods for producing the biosensor sensor measurement systems using the biosensor. A biosensor may comprise transparent rod 2; metallic reflector 40 formed on end surface 2a of one end of transparent rod 2; metallic thin film 3 formed on the outer circumferential surface of said one end of transparent rod 2; and organic substance layer 4 comprising a photo immobilizing agent containing a photo cross linking agent and a substance to be immobilized which is formed on the metallic thin film 3 on the outer circumferential surface and immobilized.

Description

technical field [0001] The present invention relates to a biosensor, a manufacturing method thereof, and a sensor detection system, in particular to the following biosensor, a manufacturing method thereof, and a sensor detection system: biological substances such as polypeptides, proteins, nucleic acids, cells, etc. are fixed on a transparent circle by a light immobilizing agent On the metal thin film on the outer peripheral side of the rod, the surface plasmon resonance phenomenon is used to detect the solution component, refractive index, protein, antibody and antigen reaction of the sample to be measured. Background technique [0002] The so-called surface plasmon resonance (Surface Plasmon Resonance, hereinafter referred to as "SPR") is an optical physical effect. When the incident light is incident on the interface between the dielectric and the metal thin film at a critical angle, a penetrating light field and a surface plasmon field will be generated. When the incide...

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Problems solved by technology

However, due to the failure to establish a method for immobilizing biological substances such as antibodies or antigens that react with the antigen or antibody of the target substance to be detected on the outer peripheral side of the optical fiber core of the sensor, even after more than ten years, as a fiber optic biological Sensors are not practical

[0005] On the other hand, as a method of immobilizing an organic substance such as an antibody or an antigen on a substrate such as a planar plate or a chip, there are known physical adsorption methods and functional group covalent bonding methods, but the above-mentioned immobilization method There is no successful precedent for immobilizing organic substances such as antibodies or antigens on the peripheral side of the optical fiber core.

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