Time-resolved fluorescence immunoassay method fro detecting Dkk-1 and kit thereof

A time-resolved fluorescence and immunoassay technology, applied in analytical materials, biological tests, measurement devices, etc., can solve the problems of poor stability, low sensitivity, and difficulty in meeting Dkk-1 detection, and achieve the effect of improving speed and high sensitivity
CN101398433AInactive Publication Date: 2009-04-01RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
Publication Date
2009-04-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a time-resolved fluoroimmunoassay used for detecting Dkk1 and a kit thereof. The fluoroimmunoassay includes the following steps: 1. solid-phase anti-body preparing; 2) europium ion marker antibody preparing; and 3) the assay method: based on the double anti-body sandwiched immunoreaction. The invention has the advantages of relatively high sensitivity, specificity and stability on detection of Dkk1; the invention also has a supermatic analytical system which can not only enhance the speed of clinical test results, but also significantly reduce the personal errors and improve the reliability of the test results.
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Description

technical field

[0001] The invention relates to an immunological detection method for secreted protein dickkopf l (Dkk-1), in particular to a time-resolved fluorescent immunoassay (TRFIA) for detecting Dkk-1 and a corresponding kit. Background technique

[0002] The Wnt signaling pathway is an evolutionarily conserved signal transduction pathway that plays an important role in controlling embryonic development, and its inappropriate activation is involved in the occurrence of various human tumors. Some recent studies have reported that gene mutations of the intracellular molecules β-catenin, APC and AXIN involved in this signaling pathway reduce the phosphorylation level of β-catenin in cells and weaken the corresponding ubiquitination degradation, resulting in stable β-catenin The combination of nuclear import and TCF / LEF regulates the expression of related genes, thus leading to the abnormal activation of wnt signaling pathway. The secreted protein dickkopf l (Dkk-1) play...

Claims

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